A curcumin analog CA-5f inhibits urokinase-type plasminogen activator and invasive phenotype of triple-negative breast cancer cells

2021 ◽  
Author(s):  
Minjoo Kim ◽  
Aree Moon
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Plasminogen Activator ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Curcumin Analog ◽  
Invasive Phenotype ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator

scholarly journals Cell Signaling by Urokinase-type Plasminogen Activator Receptor Induces Stem Cell–like Properties in Breast Cancer Cells

2010 ◽  
Vol 70 (21) ◽  
pp. 8948-8958 ◽  
Author(s):  
Minji Jo ◽  
Boryana M. Eastman ◽  
Drue L. Webb ◽  
Konstantin Stoletov ◽  
Richard Klemke ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Cell Signaling ◽  
Cancer Cells ◽  
Plasminogen Activator ◽  
Breast Cancer Cells ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator ◽  
Plasminogen Activator Receptor

Endogenously produced urokinase-type plasminogen activator is a major determinant of the basal level of activated ERK/MAP kinase and prevents apoptosis in MDA-MB-231 breast cancer cells

2001 ◽  
Vol 114 (18) ◽  
pp. 3387-3396
Author(s):  
Zhong Ma ◽  
Donna J. Webb ◽  
Minji Jo ◽  
Steven L. Gonias
Keyword(s):  
Breast Cancer ◽  
Cell Growth ◽  
Cancer Cells ◽  
Plasminogen Activator ◽  
Specific Antibody ◽  
Breast Cancer Cells ◽  
Feedback Loop ◽  
Erk Signaling ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator

Urokinase-type plasminogen activator (uPA) binds to the uPA receptor (uPAR) and activates the Ras-extracellular signal-regulated kinase (ERK) signaling pathway in many different cell types. In this study, we demonstrated that endogenously produced uPA functions as a major determinant of the basal level of activated ERK in MDA-MB-231 breast cancer cells. When these cells were cultured in the presence of antibodies that block the binding of uPA to uPAR, the level of phosphorylated ERK decreased substantially. Furthermore, conditioned medium from MDA-MB-231 cells activated ERK in MCF-7 cells and this response was blocked by uPA-specific antibody. The mitogen-activated protein kinase kinase inhibitor, PD098059, decreased expression of uPA and uPAR in MDA-MB-231 cells. Thus, uPA and the uPAR-ERK signaling pathway form a positive feedback loop in these cells. When this feedback loop was disrupted with uPA- or uPAR-specific antibody, uPA mRNA-specific antisense oligodeoxynucleotides or PD098059, cell growth was inhibited and apoptosis was promoted, as determined by the increase in cytoplasmic nucleosomes and caspase-3 activity. Treating the cells simultaneously with PD098059 and uPA- or uPAR-specific antibody did not further promote apoptosis, compared with either reagent added separately, supporting the hypothesis that uPAR and ERK are components of the same cell growth/survival-regulatory pathway. The ability of uPA to signal through uPAR, maintain an elevated basal level of activated ERK and inhibit apoptosis represents a novel mechanism whereby the uPA-uPAR system may affect breast cancer progression in vivo.

scholarly journals Urokinase-type plasminogen activator induces proliferation in breast cancer cells

2006 ◽  
Author(s):  
Mukesh Gandhari ◽  
Norbert Arens ◽  
Meher Majety ◽  
Alexandra Dorn-Beineke ◽  
Ralf Hildenbrand
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Plasminogen Activator ◽  
Breast Cancer Cells ◽  
Type Plasminogen Activator ◽  
Urokinase Type Plasminogen Activator
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