Phospholipid metabolism in kidney. II. Potassium uptake, lipid composition, and P32 labelling of the phospholipids in rabbit kidney cortex slices in vitro, and the effects of diphenylhydantoin

1963 ◽  
Vol 103 (1) ◽  
pp. 66-73 ◽  
Author(s):  
David O. Tinker ◽  
Alan Koch ◽  
Donald J. Hanahan
Keyword(s):  
Lipid Composition ◽  
Phospholipid Metabolism ◽  
Potassium Uptake ◽  
Rabbit Kidney ◽  
Kidney Cortex ◽  
Cortex Slices ◽  
Kidney Cortex Slices

Effects of DOCA, K loading, and cations on K uptake by rabbit kidney cortex

1962 ◽  
Vol 203 (6) ◽  
pp. 1001-1004 ◽  
Author(s):  
W. O. Berndt ◽  
D. A. LeSher
Keyword(s):  
External Source ◽  
Potassium Uptake ◽  
Metabolic Inhibitors ◽  
Secretory Process ◽  
Rabbit Kidney ◽  
Kidney Cortex ◽  
Fresh Tissue ◽  
Renal Handling ◽  
Cortex Slices

In previous studies the nature of the substrate requirements, as well as the effects of metabolic inhibitors on potassium uptake by rabbit renal cortex slices, have been examined. In this study a variety of factors have been examined, some of which are known to influence the renal handling of potassium in the intact animal. Pretreatment with deoxycorticosterone acetate enhanced the ability of renal slices to take up potassium from a low external source. This was true even though the fresh-tissue levels of potassium were not markedly altered. Pretreatment with potassium chloride elevated the fresh-tissue potassium level but had no effect on potassium uptake by the slices. Two organic bases which have been reported to interact with the potassium secretory process in the dog were found to have no effect on potassium uptake in vitro. Ammonium chloride was found to depress potassium uptake when present in a concentration equimolar to that of potassium.

Effect of Pentobarbital Sodium on Uptake of PAH by Rat Kidney Cortex Slices in Vitro

1958 ◽  
Vol 195 (2) ◽  
pp. 343-346 ◽  
Author(s):  
E. J. Støren
Keyword(s):  
Oxygen Consumption ◽  
Renal Cortex ◽  
Rat Kidney ◽  
Kidney Cortex ◽  
Tissue Respiration ◽  
Rat Kidney Cortex ◽  
Pentobarbital Sodium ◽  
Cortex Slices ◽  
Kidney Cortex Slices

Active uptake of PAH by rat renal cortex slices was studied by the method of Cross and Taggart. Uptake was determined at low and at high medium concentrations of PAH. Pentobarbital sodium in concentrations comparable to those found in plasma during anesthesia, significantly depressed the uptake of PAH on all occasions. Simultaneously oxygen consumption was reduced. Acetate failed to stimulate PAH uptake in the presence of pentobarbital, although tissue respiration was restored to normal.

Effect of osmotic shocks on rabbit kidney cortex slices

1983 ◽  
Vol 244 (6) ◽  
pp. F696-F705
Author(s):  
R. Gilles ◽  
C. Duchene ◽  
I. Lambert
Keyword(s):  
Cell Volume ◽  
Volume Regulation ◽  
Rabbit Kidney ◽  
Kidney Cortex ◽  
Regulation Mechanism ◽  
Kidney Slices ◽  
Regulation Process ◽  
Maximum Cell ◽  
Cortex Slices ◽  
Kidney Cortex Slices

Rabbit kidney cortex slices behave an osmometers when withstanding hyperosmotic or hyposmotic shocks of amplitude up to pi 1/pi 2 = 1.25. For hyposmotic shocks of amplitude larger than or equal to pi 1/pi 2 = 1.50, the maximum swelling achieved is less than what can be expected on the basis of the van't Hoff relation, thereby indicating that a volume regulation process is taking place. Volume regulation in kidney slices can be dissociated into two distinct phases. The first one, of swelling limitation, is very rapid and keeps maximum cell volume at values lower than expected when the tissue is considered as an osmometer. This phase is followed by a slow volume readjustment process during which volume progressively decreases towards control values. The major intracellular osmotic effector loss during both swelling limitation and volume readjustment is Na+. The overall volume regulation process is insensitive to furosemide, vanadate, and bumetanide. Swelling limitation is blocked by addition of ouabain. Contrary to what has been believed previously, there is, however, no need to implicate control of the activity of a ouabain-sensitive, Na+/K+ pump in the Na-dependent volume regulation mechanism.

Tamm—Horsfall Glycoprotein Release from Rat Kidney Cortex Slices in Vitro

1984 ◽  
Vol 67 (5) ◽  
pp. 529-534 ◽  
Author(s):  
P. K. Wirdnam ◽  
R. D. G. Milner
Keyword(s):  
Cell Membrane ◽  
Ethacrynic Acid ◽  
Rat Kidney ◽  
Kidney Cortex ◽  
Rat Kidney Cortex ◽  
The Media ◽  
Cortex Slices ◽  
Kidney Cortex Slices ◽  
Krebs Henseleit Buffer

1. Rat kidney cortex slices were incubated for 30 min at 37°C in unmodified Krebs-Henseleit buffer containing aldosterone, vasopressin, theophylline, ethacrynic acid, frusemide, spironolactone or ouabain. 2. Tamm—Horsfall glycoprotein (THG) released into the media was measured by radioimmunoassay and at the end of each experiment the slices were homogenized and assayed for THG content. 3. Incubation of kidney cortex slices in unmodified buffer resulted in a significant increase in the slice THG content when compared with pre-incubation levels. The increase was prevented by puromycin or cycloheximide. 4. Incubation in ethacrynic acid (1 mmol/l) or frusemide (10 mmol/l) resulted in a significant increase in release of THG when compared with unmodified buffer. Puromycin or cycloheximide failed to prevent the increased release. 5. THG release induced by ethacrynic acid or frusemide is probably the result of an aggregation-disaggregation reaction on the cell membrane. It is suggested that the action of the chloride inhibiting diuretics, ethacrynic acid and frusemide, is mediated in some way via THG.

THE EFFECT OF EPINEPHRINE AND COMPETITION BETWEEN MALES ON THE IN VITRO METABOLISM OF RABBIT TESTIS

1964 ◽  
Vol 42 (4) ◽  
pp. 527-532 ◽  
Author(s):  
L. L. Ewing ◽  
D. J. Noble ◽  
K. E. Ebner
Keyword(s):  
Metabolic Activity ◽  
Lactic Acid Production ◽  
Cholesterol Content ◽  
Weight Percent ◽  
Kidney Cortex ◽  
Testis Weight ◽  
Epinephrine Injection ◽  
Cortex Slices ◽  
Kidney Cortex Slices

An investigation was conducted to test the effect of stress for periods of 3, 5, and 10 days on metabolic activity in vitro, testis weight and morphology and protein, ribonucleic acid (RNA), deoxyribonucleic, acid (DNA) and cholesterol content of the testis. The two stressors (epinephrine injection and competition between males) caused an increased oxygen uptake in vitro in both testis and kidney cortex slices. This was associated with decreased glucose uptake and no change in lactic acid production in both tissues. There was no significant change in protein, RNA, DNA, and cholesterol content of testis due to stress within the 10 day period. Also, the stressors did not alter significantly testis weight, percent dry matter, or morphology. These data indicate that stress for 10 days does not have any significant effect on testis morphology and size but does cause significant alterations in metabolic activity of testis and kidney cortex slices.

scholarly journals RELEASE OF RENIN FROM DOG KIDNEY CORTEX SLICES IN VITRO

1967 ◽  
Vol 17 (4) ◽  
pp. 685-686 ◽  
Author(s):  
KENJIRO YAMAMOTO ◽  
HIROSHI TANAKA ◽  
KUNISUKE HORIUCHI ◽  
JURO UEDA
Keyword(s):  
Kidney Cortex ◽  
Dog Kidney ◽  
Cortex Slices ◽  
Kidney Cortex Slices

scholarly journals Characteristics of Ion Transport in Kidney Cortex of Mammalian Hibernators

1966 ◽  
Vol 49 (6) ◽  
pp. 1221-1239 ◽  
Author(s):  
J. S. WILLIS
Keyword(s):  
Low Temperature ◽  
Initial Rate ◽  
Ground Squirrels ◽  
Metabolic Inhibitors ◽  
Kidney Cortex ◽  
Cortex Slices ◽  
State Concentration ◽  
Kidney Cortex Slices ◽  
K Transport

Slices of kidney cortex of two species of hibernating mammals (hamsters and ground squirrels) have been leached of K, and their subsequent ability to reaccumulate K in vitro has been determined at temperatures between 38° and 0°C. At 5°C (body temperature of a hibernating mammal) uptake is appreciable in kidney cortex of both species. In the kidney cortex of hamsters, for example, the tissue K of slices incubated at 5°C reaches the same steady-state concentration after 2 hours that is observed in slices at 38°C after 20 minutes. At 0°C there is also a measurable uptake. This K transport is blocked by metabolic inhibitors and, in ground squirrel kidneys, by ouabain. In kidney cortex slices from guinea pigs net K accumulation is slight at 5°C and absent at 0°C. The initial rapid uptake of K at 38°C occurs at the same rate in kidney cortex slices of hamsters as in those of rabbits. Lowering the temperature of incubation decreases this initial rate of uptake in hamster kidney slices with a Q10 of 1.8 between 38° and 15° and of 5.7 between 15° and 0°C. In hamsters this uptake of K has been shown to require the outward extrusion of Na. Conversely, about half of the outward extrusion of Na requires K in the medium, while the remainder appears to be independent of K. The conclusions warranted are that kidney cells of hibernators possess an unusual ability to transport ions at low temperature, that this ability does not depend upon a more rapid rate at higher temperatures, and that the characteristics of transport at low temperature are qualitatively similar to those at 38°C in cells of nonhibernators.

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