Affinity labeling of a cysteine at or near the catalytic center of Escherichia coli B DNA-dependent RNA polymerase

1980 ◽  
Vol 612 (1) ◽  
pp. 286-294 ◽  
Author(s):  
Jeffrey A. Miller ◽  
Gary F. Serio ◽  
John L. Bear ◽  
Robert A. Howard ◽  
Aubrey P. Kimball
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Affinity Labeling ◽  
Catalytic Center ◽  
Escherichia Coli B

scholarly journals Trigger loop folding determines transcription rate of Escherichia coli’s RNA polymerase

2014 ◽  
Vol 112 (3) ◽  
pp. 743-748 ◽  
Author(s):  
Yara X. Mejia ◽  
Evgeny Nudler ◽  
Carlos Bustamante
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Single Molecule ◽  
Conformational Changes ◽  
Elongation Rate ◽  
Nucleoside Triphosphate ◽  
Transcription Rate ◽  
Nucleotide Analogs ◽  
Catalytic Center ◽  
Trigger Loop

Two components of the RNA polymerase (RNAP) catalytic center, the bridge helix and the trigger loop (TL), have been linked with changes in elongation rate and pausing. Here, single molecule experiments with the WT and two TL-tip mutants of the Escherichia coli enzyme reveal that tip mutations modulate RNAP’s pause-free velocity, identifying TL conformational changes as one of two rate-determining steps in elongation. Consistent with this observation, we find a direct correlation between helix propensity of the modified amino acid and pause-free velocity. Moreover, nucleotide analogs affect transcription rate, suggesting that their binding energy also influences TL folding. A kinetic model in which elongation occurs in two steps, TL folding on nucleoside triphosphate (NTP) binding followed by NTP incorporation/pyrophosphate release, quantitatively accounts for these results. The TL plays no role in pause recovery remaining unfolded during a pause. This model suggests a finely tuned mechanism that balances transcription speed and fidelity.

scholarly journals STREPTOVARICIN- AND RIFAMPIGIN-RESISTANGE OF RNA POLYMERASE IN A RESISTANT CLONE OF ESCHERICHIA COLI B

1968 ◽  
Vol 21 (8) ◽  
pp. 521-522 ◽  
Author(s):  
KAZUO NITTA ◽  
SATOSHI MIZUNO ◽  
HISAJI YAMAZAKI ◽  
HAMAO UMEZAWA
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Resistant Clone ◽  
Escherichia Coli B

Affinity labeling of escherichia coli DNA-dependent RNA polymerase with 5-formyl-1-(α-D-ribofuranosyl)uracil 5'-triphosphate

Biochemistry ◽  
1976 ◽  
Vol 15 (10) ◽  
pp. 2086-2091 ◽  
Author(s):  
Victor W. Armstrong ◽  
Hans Sternbach ◽  
Fritz Eckstein
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Affinity Labeling

Subunit localizations of zinc(II) in DNA-dependent RNA polymerase from Escherichia coli B

1979 ◽  
Vol 579 (2) ◽  
pp. 291-297 ◽  
Author(s):  
Jeffrey A. Miller, ◽  
Gary F. Serio, ◽  
Robert A. Howard ◽  
John L. Bear ◽  
John E. Evans ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Escherichia Coli B
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