A heparin-binding erythroid cell stimulating factor from fetal bovine serum has the N-terminal sequence of insulin-like growth factor II

1990 ◽  
Vol 166 (2) ◽  
pp. 557-561 ◽  
Author(s):  
Qinggang Li ◽  
Russell Blacher ◽  
Fred Esch ◽  
L.Fernando Congote
Keyword(s):  
Growth Factor ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Erythroid Cell ◽  
Insulin Like Growth Factor ◽  
Heparin Binding ◽  
Terminal Sequence ◽  
Factor Ii ◽  
Fetal Bovine ◽  
Stimulating Factor

scholarly journals Accurate processing and secretion in the baculovirus expression system of an erythroid-cell-stimulating factor consisting of a chimaera of insulin-like growth factor II and an insect insulin-like peptide

1994 ◽  
Vol 299 (1) ◽  
pp. 101-107 ◽  
Author(s):  
L F Congote ◽  
Q Li
Keyword(s):  
Amino Acids ◽  
Growth Factor ◽  
Insect Cells ◽  
Expression System ◽  
Recombinant Baculovirus ◽  
Erythroid Cell ◽  
Insulin Like Growth Factor ◽  
Terminal Sequence ◽  
Factor Ii ◽  
Terminal Amino

A synthetic gene encoding the signal peptide and the N-terminal sequence of bombyxin, an insect insulin-like peptide, and the 58 amino acids of the C-terminal sequence of human insulin-like growth factor II (IGF II) has been expressed using the baculovirus system. This synthetic chimaera was obtained by amplification of four overlapping oligonucleotides using Taq polymerase and cloning into the transfer vector pBluebac. The construct was integrated by homologous recombination into the Autographa californica nuclear polyhedrosis genome. Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus secreted an accurately processed peptide consisting of the ten N-terminal amino acids of bombyxin and the 58 C-terminal amino acids of IGF II. The N-terminal glutamine of bombyxin was changed to asparagine to facilitate sequencing of the synthetic peptide. The chimaera was five times more potent than human recombinant IGF II in its capacity to stimulate thymidine incorporation into erythroid cells of fetal bovine liver in a serum-free medium. It stimulated erythroid colony formation in the presence of 2 microunits/ml erythropoietin in cells cultured over a monolayer of stromal cells of fetal liver. Artificial chimaeras as described here may prove useful for the production of insulin, IGF I and other peptides as secreted proteins in insect cells.

scholarly journals 155 EXPRESSION OF THE INSULIN-LIKE GROWTH FACTOR-II/MANNOSE-6-PHOSPHATE RECEPTOR IN FETAL BOVINE TISSUES

1991 ◽  
Vol 30 (6) ◽  
pp. 654-654
Author(s):  
Maria Pluender ◽  
Helga Sauerwein ◽  
Barbara Funk ◽  
Ulrike Kessler ◽  
Hans-Peter Schwarz ◽  
...  
Keyword(s):  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
Factor Ii ◽  
Fetal Bovine ◽  
Mannose 6 Phosphate
Sign in / Sign up

Export Citation Format

Share Document