In vitro phosphorylation and the identification of multiple protein changes in membranes of Chinese hamster lung cells resistant to adriamycin

The purpose of this study was to test the type and the degree of cytotoxic effects of epirubicin and radiation in combined treatment on Chinese hamster lung cells in vitro. Experiments were performed with proliferating tissue culture cells. Cell kill was determined by colony-forming ability. The maximum killing effects were obtained when simultaneous action of drug treatment and irradiation occurred. Their interaction was synergistic. Synergism depended on time of drug incubation (epirubicin present for 1 h). Slight antagonism was noted after prolonged drug action (24 h).

Download Full-text

The genotoxity of by-products by chlorination and ozonation of the river water in the presence of bromide ions

Water Science & Technology ◽

10.2166/wst.2000.0389 ◽

2000 ◽

Vol 42 (3-4) ◽

pp. 259-264 ◽

Cited By ~ 12

Author(s):

T. Nobukawa ◽

S. Sanukida

Keyword(s):

Chinese Hamster ◽

Chloride Ions ◽

Lung Cells ◽

Resin Column ◽

River Waters ◽

Drinking Waters ◽

Bromide Ions ◽

Chromosomal Aberration Test ◽

By Products

This study focused on the evaluation of the genotoxic characteristics of chlorinated and brominated substances produced by ozonation and chlorination of the river waters containing bromide ions. The mutagenic activities of drinking waters and disinfected river waters were measured by Ames tests using S. typhimurium strains TA100 and TA98, and by chromosomal aberration test using cultured Chinese hamster lung cells (CHL/IU) in vitro. After flowing samples through CSP800 or XAD-2 resin column, absorbed substances dissolved in dimethyl sulfoxide, and submitted to the mutagenicity assays. Correlation was high between water qualities revealed by chemical analysis and mutagenic potentials. Mutagenic activities of the drinking waters produced by chlorination were observed to be higher than those by ozonation. Chlorinated waters highly increased in cells with the exchange- and chromatid-type of chromosomal aberrations compared with unchlorinated waters. The increase of mutagenic potentials was caused by by-products when bromide ions were substituted for chloride ions with various ratios.

Download Full-text

Effects of 7H-pyridocarbazole mono and bifunctional DNA-intercalators on chinese hamster lung cells in vitro

Biochemical Pharmacology ◽

10.1016/0006-2952(84)90157-6 ◽

1984 ◽

Vol 33 (22) ◽

pp. 3681-3688 ◽

Cited By ~ 10

Author(s):

Jean-Pierre Bendirdjian ◽

Charlotte Delaporte ◽

Bernard P. Roques ◽

Alain Jacquemin-Sablon

Keyword(s):

Chinese Hamster ◽

Lung Cells ◽

Dna Intercalators

Download Full-text

Ultrastructural Changes in Three Different Mammalian Cells Following Ultraviolet Laser Irradiation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100095017 ◽

1972 ◽

Vol 30 ◽

pp. 350-351

Author(s):

K. Shankar Narayan ◽

Kailash C. Gupta ◽

Tohru Okigaki

Keyword(s):

Ultraviolet Light ◽

Mammalian Cells ◽

Biological Effects ◽

Survival Rates ◽

Chinese Hamster ◽

Cell Types ◽

Differential Sensitivity ◽

Ultrastructural Changes ◽

Ultraviolet Laser

The biological effects of short-wave ultraviolet light has generally been described in terms of changes in cell growth or survival rates and production of chromosomal aberrations. Ultrastructural changes following exposure of cells to ultraviolet light, particularly at 265 nm, have not been reported.We have developed a means of irradiating populations of cells grown in vitro to a monochromatic ultraviolet laser beam at a wavelength of 265 nm based on the method of Johnson. The cell types studies were: i) WI-38, a human diploid fibroblast; ii) CMP, a human adenocarcinoma cell line; and iii) Don C-II, a Chinese hamster fibroblast cell strain. The cells were exposed either in situ or in suspension to the ultraviolet laser (UVL) beam. Irradiated cell populations were studied either "immediately" or following growth for 1-8 days after irradiation.Differential sensitivity, as measured by survival rates were observed in the three cell types studied. Pattern of ultrastructural changes were also different in the three cell types.

Download Full-text