Studies on the electron-transport system XXVII. The respiratory activity of acetone-extracted beef-heart mitochondria role of coenzyme Q and other lipids

1961 ◽  
Vol 47 (2) ◽  
pp. 358-377 ◽  
Author(s):  
Robert L. Lester ◽  
Sidney Fleischer
Keyword(s):  
Electron Transport ◽  
Transport System ◽  
Respiratory Activity ◽  
Coenzyme Q ◽  
Electron Transport System ◽  
Heart Mitochondria ◽  
Beef Heart ◽  
Beef Heart Mitochondria

The inhibition of mammalian mitochondrial NADU oxidation by chloramphenicol and its isomers and analogues

1968 ◽  
Vol 46 (9) ◽  
pp. 1003-1008 ◽  
Author(s):  
K. B. Freeman ◽  
D. Haldar
Keyword(s):  
Cytochrome B ◽  
Respiratory Chain ◽  
Rat Liver ◽  
Nadh Dehydrogenase ◽  
Coenzyme Q ◽  
Liver Mitochondria ◽  
Heart Mitochondria ◽  
Rat Liver Mitochondria ◽  
Beef Heart ◽  
Beef Heart Mitochondria

Chloramphenicol and its isomers and analogues have been found to inhibit the oxidation of NADH, but not that of succinate, by beef heart mitochondria. They must therefore inhibit the NADH dehydrogenase segment of the respiratory chain. Chloramphenicol gave 50% inhibition at a concentration of 1 mM. The methylthio analogue of chloramphenicol inhibited NADH – coenzyme Q6 reductase but not NADH–ferricyanide reductase. Spectrophotometric observations suggest that these inhibitors act between NADH and flavin in coupled rat liver mitochondria and between flavin and cytochrome b in uncoupled beef heart mitochondria.

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