Reserve bilirubin binding capacity assessed by difference spectroscopy: Assay statistics and results on newborn sera

1980 ◽  
Vol 104 (3) ◽  
pp. 309-318 ◽  
Author(s):  
K.Owen Ash ◽  
Wayne M. Hentschel ◽  
Gary M. Chan ◽  
James T. Wu
Keyword(s):  
Binding Capacity ◽  
Difference Spectroscopy ◽  
Bilirubin Binding

Bilirubin-protein interactions monitored by difference spectroscopy.

1978 ◽  
Vol 24 (9) ◽  
pp. 1491-1495 ◽  
Author(s):  
K O Ash ◽  
M Holmer ◽  
C S Johnson
Keyword(s):  
Protein Interactions ◽  
Binding Capacity ◽  
Research Effort ◽  
Difference Spectrum ◽  
Molar Ratio ◽  
Assay Method ◽  
Difference Spectroscopy ◽  
Difference Spectra ◽  
The Difference ◽  
Bilirubin Binding

Abstract Difference spectroscopy is used to monitor bilirubin-protein interactions, to assess the residual binding capacity of proteins for bilirubin. A change in the difference spectra monitored at 482 nm is directly proportional to bound bilirubin up to a molar ratio of bilirubin to albumin of approximately 1; increasing bilirubin beyond the 1:1 molar ratio does not further change the difference spectra. After excess free bilirubin is added, the change in the difference spectrum is proportional to the residual binding capacity of the serum for bilirubin. The risk of kernicterus among neonates may be assessed by monitoring the residual bilirubin binding capacity of serum. This report summarizes our research effort leading to an assay method which requires only 40 microliter of serum and can be completed in less than 10 min.

Unbound Bilirubin and Kernicterus in Low-Birth-Weight Infants

PEDIATRICS ◽  
1982 ◽  
Vol 69 (4) ◽  
pp. 481-485 ◽  
Author(s):  
William J. Cashore ◽  
William Oh
Keyword(s):  
Preterm Infants ◽  
Binding Capacity ◽  
Assisted Ventilation ◽  
Albumin Concentration ◽  
Low Birth Weight Infants ◽  
Indirect Bilirubin ◽  
Clinical Complications ◽  
Horseradish Peroxidase Method ◽  
Unbound Bilirubin ◽  
Bilirubin Binding

Unbound bilirubin, bilirubin binding capacity, and bilirubin binding affinity were determined by the horseradish peroxidase method at the time of maximum hyperbilirubinemia and/or before exchange transfusions in 13 preterm infants who later died and had autopsies performed. Five of the 13 infants had kernicterus at autopsy. There were no significant differences in weight, gestational age, highest indirect bilirubin level, albumin concentration, severity of acidosis, use of assisted ventilation, sepsis, or other major clinical complications between the five infants with kernicterus and the eight infants without kernicterus. Compared with the eight nonkernicteric infants, the five kernicteric infants had significantly higher unbound bilirubin concentrations (13 ± 10 vs 27 ± 9 nmoles/liter, respectively, P < .05) and significantly lower bilirubin binding capacity and affinity. The data suggest an association between low bilirubin binding capacity and affinity, increased unbound bilirubin, and kernicterus in preterm infants with severe clinical complications.

Decreased Bilirubin-Binding Capacity in Uremic Serum Caused by an Accumulation of Furan Dicarboxylic Acid

Nephron ◽  
2000 ◽  
Vol 85 (1) ◽  
pp. 60-64 ◽  
Author(s):  
Yasuhiro Tsutsumi ◽  
Toru Maruyama ◽  
Akira Takadate ◽  
Hidekata Shimada ◽  
Masaki Otagiri
Keyword(s):  
Dicarboxylic Acid ◽  
Binding Capacity ◽  
Uremic Serum ◽  
Bilirubin Binding

A multi-center evaluation of a device for measurement of bilirubin binding capacity in neonates: the effects of gestational age, Intralipid exposure and illness severity

2019 ◽  
Vol 39 (6) ◽  
pp. 883-888
Author(s):  
David L. Schutzman ◽  
Vinod K. Bhutani ◽  
Martin E Castillo Cuadrado ◽  
Angelo A Lamola ◽  
Ivan Frantz ◽  
...  
Keyword(s):  
Gestational Age ◽  
Binding Capacity ◽  
Illness Severity ◽  
Bilirubin Binding
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