Correlation between ultrastructural aspect of nucleoli and inhibition of ribosomal RNA synthesis in Xenopus laevis oocytes

1972 ◽  
Vol 74 (2) ◽  
pp. 547-551 ◽  
Author(s):  
Chr. Thomas
Development ◽  
1974 ◽  
Vol 32 (2) ◽  
pp. 515-532
Author(s):  
A. Colman

RNA synthesis can be maintained in large oocytes of Xenopus laevis during periods of in vitro culture of at least 10 days. A simple salt medium, modified Barth's solution, is found to be as effective a culture medium for these oocytes as several other complex media. The newly synthesized RNA is characterized electrophoretically and shown to consist predominantly of ribosomal RNA precursor, 28S and 18S ribosomal RNA, and 4S RNA. The distribution of this RNA within the oocyte is detected autoradiographically, where it is found to be greatly concentrated over the nucleoli. No qualitative alterations in either of these parameters are found during culture, within the limits of sensitivity of the assay procedures.


1977 ◽  
Vol 57 (1) ◽  
pp. 210-214 ◽  
Author(s):  
K. Shiokawa ◽  
A. Kawahara ◽  
Y. Misumi ◽  
Y. Yasuda ◽  
K. Yamana

1968 ◽  
Vol 52 (1) ◽  
pp. 252-260 ◽  
Author(s):  
K. Wada ◽  
K. Shiokawa ◽  
K. Yamana

1973 ◽  
Vol 33 (2) ◽  
pp. 241-248 ◽  
Author(s):  
R.A. Laskey ◽  
J.C. Gerhart ◽  
J.S. Knowland

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