Mitochondrial RNA synthesis in rat liver

Cryo-EM structures of transcription pre-initiation complex (PIC) and initiation complex (IC) of yeast mitochondrial RNA polymerase show fully resolved transcription bubbles and explain promoter melting, template alignment, DNA scrunching, transition into elongation, and abortive synthesis. Promoter melting initiates in PIC with MTF1 trapping the −4 to −2 non-template (NT) bases in its NT-groove. Transition to IC is marked by a large-scale movement that aligns the template with RNA at the active site. RNA synthesis scrunches the NT strand into an NT-loop, which interacts with centrally positioned MTF1 C-tail. Steric clashes of the C-tail with RNA:DNA and NT-loop, and dynamic scrunching-unscrunching of DNA explain abortive synthesis and transition into elongation. Capturing the catalytically active IC-state with UTPαS poised for incorporation enables modeling toxicity of antiviral nucleosides/nucleotides.

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Studies on in vitro RNA Synthesis by RNA Polymerases from Rat Liver Tissue

Regulation of Transcription and Translation in Eukaryotes ◽

10.1007/978-3-642-65725-2_8 ◽

1973 ◽

pp. 139-162 ◽

Cited By ~ 1

Author(s):

K. H. Seifart ◽

A. Ferencz ◽

B. Benecke

Keyword(s):

Rat Liver ◽

Liver Tissue ◽

Rna Synthesis ◽

Rna Polymerases

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Effect of Cycloheximide on the Nucleolar RNA Synthesis in Rat Liver

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a131783 ◽

1977 ◽

Vol 82 (4) ◽

pp. 1109-1119 ◽

Cited By ~ 10

Author(s):

Toshio ONISHI ◽

Takashi MATSUI ◽

Masami MURAMATSU

Keyword(s):

Rat Liver ◽

Rna Synthesis ◽

Nucleolar Rna

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EFFECT OF 3′:5′-CYCLIC AMP ON NUCLEAR AND MITOCHONDRIAL RNA SYNTHESIS OF RAT ADRENOCORTICAL CELLS

Acta Endocrinologica ◽

10.1530/acta.0.0700081 ◽

1972 ◽

Vol 70 (1) ◽

pp. 81-88 ◽

Cited By ~ 14

Author(s):

G. G. Nussdorfer ◽

G. Mazzocchi

Keyword(s):

Cyclic Amp ◽

Rna Synthesis ◽

Mitochondrial Rna ◽

Adrenocortical Cells ◽

Hypophysectomized Rats ◽

High Resolution Autoradiography ◽

Intracellular Mediators ◽

Stimulation Of ◽

Trophic Action

ABSTRACT The effect of 3′:5′-cyclic AMP (cAMP) on the incorporation of 3H-uridine into adrenocortical cells of hypophysectomized rats was investigated by high resolution autoradiography. The quantitative analysis of autoradiographs shows that cAMP, like ACTH, enhances the tracer incorporation into both nuclei and mitochondria. These findings are discussed in relation to the results of various investigations, indicating that cAMP functions as an intracellular mediator of the long-term trophic action of ACTH on the adrenal cortex. It is suggested that the mechanism of this action of cAMP consists in the stimulation of both nuclear and mitochondrial RNA synthesis. Furthermore, the possibility that the trophic action of ACTH on the adrenal gland involves other intracellular mediators, as well as cAMP, is suggested.

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