Immunofluorescence microscopy of the intracellular translocation of glucocorticoid-receptor complexes in rat hepatoma (HTC) cells

1980 ◽  
Vol 127 (2) ◽  
pp. 293-297 ◽  
Author(s):  
M.V. Govindan
Keyword(s):  
Glucocorticoid Receptor ◽  
Immunofluorescence Microscopy ◽  
Receptor Complexes ◽  
Htc Cells ◽  
Intracellular Translocation ◽  
Rat Hepatoma

scholarly journals Glucocorticoid regulation of amino acid transport in anucleate rat hepatoma (HTC) cells.

1981 ◽  
Vol 88 (3) ◽  
pp. 536-542 ◽  
Author(s):  
R A McDonald ◽  
T D Gelehrter
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Kinetic Properties ◽  
Acid Transport ◽  
Intact Cells ◽  
Receptor Complexes ◽  
Htc Cells ◽  
Regulation Of Transport ◽  
Rat Hepatoma

The transport of alpha-aminoisobutyric acid (AIB) by rat hepatoma tissue culture (HTC) cells is rapidly and reversibly inhibited by dexamethasone and other glucocorticoids. To investigate the role of the nucleus in the regulation of transport and to determine whether steroid hormones or steroid-receptor complexes may have direct effects on cytoplasmic or membrane functions, we have examined the regulation of transport by dexamethasone in anucleate HTC cells. Cytoplasts prepared from suspension cultures of HTC cells fully retain active transport of AIB with the same kinetic properties as intact cells. However, the uptake of AIB is not inhibited by dexamethasone or other corticosteroids. Neither is the inhibited rate of transport, manifested by cytoplasts prepared from dexamethasone-treated cells, restored to normal upon removal of the hormone. Anucleate cells exhibit specific, saturable binding of [3H]dexamethasone; however, the binding is reduced compared with that of intact cells. The nucleus is thus required for the glucocorticoid regulation of amino acid transport in HTC cells.

Glucocorticoid-receptor complexes are associated with small RNA in vitro

1989 ◽  
Vol 32 (5) ◽  
pp. 633-642 ◽  
Author(s):  
Gian Paolo Rossini ◽  
Ann-Charlotte Wikström ◽  
Jan-Åke Gustafsson
Keyword(s):  
Glucocorticoid Receptor ◽  
Small Rna ◽  
Receptor Complexes

Tight binding of glucocorticoid-receptor complexes to histone-agarose

1988 ◽  
Vol 151 (2) ◽  
pp. 763-767 ◽  
Author(s):  
Kiyotaka Ueda ◽  
Fumihide Isohashi ◽  
Kazuki Okamoto ◽  
Ikuo Kokuhu ◽  
Kotohiko Kimura ◽  
...  
Keyword(s):  
Glucocorticoid Receptor ◽  
Tight Binding ◽  
Receptor Complexes
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