scholarly journals In vitro synthesis of yeast ribosomal proteins

FEBS Letters ◽  
1975 ◽  
Vol 58 (1-2) ◽  
pp. 219-221 ◽  
Author(s):  
W.H. Mager ◽  
R. Hoving ◽  
R.J. Planta
Keyword(s):  
Ribosomal Proteins ◽  
In Vitro Synthesis

scholarly journals Studies on the Role of Ribosomal Proteins L7 and L12 in the in Vitro Synthesis of β-Galactosidase

1973 ◽  
Vol 248 (14) ◽  
pp. 5012-5015
Author(s):  
Hsiang-Fu Kung ◽  
J. Eugene Fox ◽  
Carlos Spears ◽  
Nathan Brot ◽  
Herbert Weissbach
Keyword(s):  
Ribosomal Proteins ◽  
In Vitro Synthesis

scholarly journals In Vitro Synthesis of Ribosomal Proteins Directed by Escherichia coli DNA

1974 ◽  
Vol 71 (2) ◽  
pp. 446-450 ◽  
Author(s):  
E. Kaltschmidt ◽  
L. Kahan ◽  
M. Nomura
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Proteins ◽  
In Vitro Synthesis

The native structure of the eukaryotic ribosome

1983 ◽  
Vol 41 ◽  
pp. 432-433
Author(s):  
R.A. Milligan ◽  
P.N.T. Unwin
Keyword(s):  
Ribosomal Proteins ◽  
Crystal Form ◽  
Native Structure ◽  
X Ray Diffraction ◽  
Eukaryotic Ribosome ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Resolution Structure ◽  
Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

In vitro synthesis of a unique RNA species by a T particle of vesicular stomatitis virus.

1977 ◽  
Vol 23 (3) ◽  
pp. 708-716 ◽  
Author(s):  
S U Emerson ◽  
P M Dierks ◽  
J T Parsons
Keyword(s):  
Vesicular Stomatitis Virus ◽  
In Vitro Synthesis ◽  
Vesicular Stomatitis

In vitro synthesis of enzymatically active HIV-1 protease for rapid phenotypic resistance profiling

2005 ◽  
Vol 32 (4) ◽  
pp. 294-299 ◽  
Author(s):  
Dieter Hoffmann ◽  
Bernd Buchberger ◽  
Cordula Nemetz
Keyword(s):  
In Vitro Synthesis ◽  
Phenotypic Resistance ◽  
Hiv 1
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