Effect of rabbit anti-pancreatic polypeptide serum on postprandial pancreatic exocrine secretion in dogs

1986 ◽  
Vol 90 (6) ◽  
pp. 1926-1931 ◽  
Author(s):  
Alphons J.L. de Jong ◽  
Manfred V. Singer ◽  
Cornelis B.H.W. Lamers
Keyword(s):  
Pancreatic Polypeptide ◽  
Exocrine Secretion ◽  
Pancreatic Exocrine Secretion ◽  
Pancreatic Exocrine

Cholecystokinin-8s excites identified rat pancreatic-projecting vagal motoneurons

2007 ◽  
Vol 293 (2) ◽  
pp. G484-G492 ◽  
Author(s):  
Shuxia Wan ◽  
F. Holly Coleman ◽  
R. Alberto Travagli
Keyword(s):  
Brain Stem ◽  
Pancreatic Polypeptide ◽  
Potassium Conductance ◽  
Input Resistance ◽  
Exocrine Secretion ◽  
Motor Nucleus ◽  
Pancreatic Exocrine Secretion ◽  
Calcium Dependent ◽  
Synaptic Circuits ◽  
Pancreatic Exocrine

It is known that cholecystokinin (CCK) acts in a paracrine fashion to increase pancreatic exocrine secretion via vagal circuits. Recent evidence, however, suggests that CCK-8s actions are not restricted to afferent vagal fibers, but also affect brain stem structures directly. Within the brain stem, preganglionic neurons of the dorsal motor nucleus of the vagus (DMV) send efferent fibers to subdiaphragmatic viscera, including the pancreas. Our aims were to investigate whether DMV neurons responded to exogenously applied CCK-8s and, if so, the mechanism of action. Using whole cell patch-clamp recordings we show that perfusion with CCK-8s induced a concentration-dependent excitation in ∼60% of identified pancreas-projecting DMV neurons. The depolarization was significantly reduced by tetrodotoxin, suggesting both direct (on the DMV membrane) and indirect (on local synaptic circuits) effects. Indeed, CCK-8s increased the frequency of miniature excitatory currents onto DMV neurons. The CCK-A antagonist, lorglumide, prevented the CCK-8s-mediated excitation whereas the CCK-B preferring agonist, CCK-nonsulfated, had no effect, suggesting the involvement of CCK-A receptors only. In voltage clamp, the CCK-8s-induced inward current reversed at −106 ± 3 mV and the input resistance increased by 150 ± 15%, suggesting an effect mediated by the closure of a potassium conductance. Indeed, CCK-8s reduced both the amplitude and the time constant of decay of a calcium-dependent potassium conductance. When tested with pancreatic polypeptide (which reduces pancreatic exocrine secretion), cells that responded to CCK-8s with an excitation were, instead, inhibited by pancreatic polypeptide. These data indicate that CCK-8s may control pancreas-exocrine secretion also via an effect on pancreas-projecting DMV neurons.

Effects of pancreatic polypeptide on the pancreatic exocrine secretion stimulated by secretin and cholecystokinin in the conscious pig

1989 ◽  
Vol 24 (1) ◽  
pp. 55-65 ◽  
Author(s):  
Annik Langlois ◽  
Tristan Corring ◽  
Jean-Claude Cuber ◽  
Anne Marie Gueugneau ◽  
Florence Levenez ◽  
...  
Keyword(s):  
Pancreatic Polypeptide ◽  
Exocrine Secretion ◽  
Pancreatic Exocrine Secretion ◽  
Pancreatic Exocrine

scholarly journals The influence of bovine pancreatic polypeptide on pancreatic exocrine secretion in young calves

1979 ◽  
Vol 19 (3B) ◽  
pp. 843-848 ◽  
Author(s):  
Marie-Jeanne DAVICCO ◽  
J. LEFAIVRE ◽  
J.-P. BARLET ◽  
R. DABERT ◽  
Bernadette LASSALAS ◽  
...  
Keyword(s):  
Pancreatic Polypeptide ◽  
Exocrine Secretion ◽  
Pancreatic Exocrine Secretion ◽  
Bovine Pancreatic Polypeptide ◽  
Pancreatic Exocrine

Effect of pancreatic polypeptide-antiserum on bombesin-stimulated pancreatic exocrine secretion in dogs

Peptides ◽  
1987 ◽  
Vol 8 (6) ◽  
pp. 973-976 ◽  
Author(s):  
Alphons J.L. de Jong ◽  
Manfred V. Singer ◽  
Cornelis B.H.W. Lamers
Keyword(s):  
Pancreatic Polypeptide ◽  
Exocrine Secretion ◽  
Pancreatic Exocrine Secretion ◽  
Pancreatic Exocrine

Role of pancreatic polypeptide in the regulation of pancreatic exocrine secretion in dogs

1988 ◽  
Vol 255 (5) ◽  
pp. G535-G541 ◽  
Author(s):  
K. Shiratori ◽  
K. Y. Lee ◽  
T. M. Chang ◽  
Y. H. Jo ◽  
D. H. Coy ◽  
...  
Keyword(s):  
Pancreatic Polypeptide ◽  
Pancreatic Secretion ◽  
Inhibitory Action ◽  
Peak Plasma ◽  
Peak Plasma Concentration ◽  
Exocrine Secretion ◽  
Pancreatic Exocrine Secretion ◽  
Pancreatic Exocrine ◽  
Human Pancreatic Polypeptide

The effect of intravenous infusion of synthetic human pancreatic polypeptide (HPP) or a rabbit anti-PP serum on pancreatic exocrine secretion was studied in 10 dogs with gastric and Thomas duodenal cannulas. The infusion of HPP, at a dose of 1 microgram.kg-1.h-1, achieved a plasma PP concentration that mimicked the peak plasma concentration of PP in both interdigestive and postprandial states. This dose of HPP significantly inhibited pancreatic secretion in the interdigestive state. By contrast, immunoneutralization of circulating PP by a rabbit anti-PP serum resulted in significant increases (P less than 0.05) in both interdigestive and postprandial pancreatic secretion, including water, bicarbonate, and protein. The increase in the pancreatic secretion paralleled a decrease in circulating PP level, which lasted for as long as 5 days. Furthermore, the anti-PP serum blocked the inhibitory action of exogenous HPP on pancreatic exocrine secretion. The present study indicates that endogenous PP plays a significant role in the regulation of the pancreatic exocrine secretion in both interdigestive and digestive states. Thus we conclude that PP is another hormone regulating pancreatic exocrine secretion in dogs.

Diurnal profile of pancreatic exocrine secretion and plasma levels of gut hormones (Cholecystokinin and Pancreatic Polypeptide)

1987 ◽  
Vol 22 (6) ◽  
pp. 748-755 ◽  
Author(s):  
Itsuro Nakano ◽  
Akihiro Funakoshi ◽  
Hirotsugu Shinozaki ◽  
Kazunori Miyazaki ◽  
Kayoko Tateishi ◽  
...  
Keyword(s):  
Pancreatic Polypeptide ◽  
Plasma Levels ◽  
Gut Hormones ◽  
Exocrine Secretion ◽  
Pancreatic Exocrine Secretion ◽  
Diurnal Profile ◽  
Pancreatic Exocrine
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