Partial duplication of the large ribosomal RNA sequence in an inverted repeat in circular mitochondrial DNA from Kloeckera africana

Physical maps of the mitochondrial DNA (mtDNA) of Hyphochytrium catenoides and Rhizidiomyces apophysatus were constructed. Hyphochytrium catenoides has a 54-kb (kilobase pair) circular mtDNA with an inverted repeat of 13.5–14.5 kb which contains the large ribosomal RNA gene at one extreme of the inverted repeat, adjacent to the small unique region. Genome complexity (genome size minus one arm of the inverted repeat) is 39.5–40.5 kb, identical with the average value for Oomycetes. Rhizidiomyces apophysatus has a 50-kb circular mtDNA with no inverted repeat. No obvious alignment of the two maps is possible. The occurrence of a typical inverted repeat in a group of fungi closely related to the Oomycetes indicates that the character is ancestral for the Oomycetes and that its modification or loss within the Oomycetes can be treated as a derived character for the purpose of phylogenetic analysis.

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First Insight into Groundwater Copepods of the Polish Lowland

Water ◽

10.3390/w13152086 ◽

2021 ◽

Vol 13 (15) ◽

pp. 2086

Author(s):

Maciej Karpowicz ◽

Sabina Smolska ◽

Magdalena Świsłocka ◽

Joanna Moroz

Keyword(s):

Mitochondrial Dna ◽

Ribosomal Rna ◽

Dna Markers ◽

Coi Gene ◽

Molecular Characteristics ◽

Morphological Description ◽

Single Plate ◽

North Eastern ◽

Insight Into

Our results are the first insight into groundwater copepods of the Polish Lowland. The sampling was conducted in 28 wells in north-eastern Poland, and Copepoda were present in 16 wells. We have identified six Copepoda species and one Cladocera. We have classified four species as stygophiles—Eucyclops serrulatus, Diacyclops bisetosus, Diacyclops crassicaudis, and Cyclops furcifer. These species were frequently found in studied wells of different regions of north-eastern Poland, often in high numbers, and females with egg sacs were observed. We present a detailed morphological description of these species, together with molecular characteristics based on mitochondrial DNA markers (COI gene) for E. serrulatus, D. bisetosus, and D. crassicaudis, and 12S ribosomal RNA for C. furcifer. We also present the development of abnormal structures in one specimen of D. crassicaudis, where the upper part of furcal rami was fused to form a single plate.

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The organization of genes in yeast mitochondrial DNA. I. The genes for large and small ribosomal RNA are far apart

Biochemical and Biophysical Research Communications ◽

10.1016/s0006-291x(75)80202-6 ◽

1975 ◽

Vol 65 (2) ◽

pp. 699-707 ◽

Cited By ~ 51

Author(s):

J.P.M. Sanders ◽

C. Heyting ◽

P. Borst

Keyword(s):

Mitochondrial Dna ◽

Ribosomal Rna ◽

Yeast Mitochondrial Dna

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Saccharomyces cerevisiae contains an RNase MRP that cleaves at a conserved mitochondrial RNA sequence implicated in replication priming.

Molecular and Cellular Biology ◽

10.1128/mcb.12.6.2561 ◽

1992 ◽

Vol 12 (6) ◽

pp. 2561-2569 ◽

Cited By ~ 37

Author(s):

L L Stohl ◽

D A Clayton

Keyword(s):

Saccharomyces Cerevisiae ◽

Mitochondrial Dna ◽

Dna Replication ◽

Rna Processing ◽

Mitochondrial Rna ◽

Rna Sequence ◽

Rnase Mrp ◽

Processing Activity ◽

Yeast Mitochondrial Dna ◽

Human And Mouse

Yeast mitochondrial DNA contains multiple promoters that sponsor different levels of transcription. Several promoters are individually located immediately adjacent to presumed origins of replication and have been suggested to play a role in priming of DNA replication. Although yeast mitochondrial DNA replication origins have not been extensively characterized at the primary sequence level, a common feature of these putative origins is the occurrence of a short guanosine-rich region in the priming strand downstream of the transcriptional start site. This situation is reminiscent of vertebrate mitochondrial DNA origins and raises the possibility of common features of origin function. In the case of human and mouse cells, there exists an RNA processing activity with the capacity to cleave at a guanosine-rich mitochondrial RNA sequence at an origin; we therefore sought the existence of a yeast endoribonuclease that had such a specificity. Whole cell and mitochondrial extracts of Saccharomyces cerevisiae contain an RNase that cleaves yeast mitochondrial RNA in a site-specific manner similar to that of the human and mouse RNA processing activity RNase MRP. The exact location of cleavage within yeast mitochondrial RNA corresponds to a mapped site of transition from RNA to DNA synthesis. The yeast activity also cleaved mammalian mitochondrial RNA in a fashion similar to that of the mammalian RNase MRPs. The yeast endonuclease is a ribonucleoprotein, as judged by its sensitivity to nucleases and proteinase, and it was present in yeast strains lacking mitochondrial DNA, which demonstrated that all components required for in vitro cleavage are encoded by nuclear genes. We conclude that this RNase is the yeast RNase MRP.

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Phylogenetic relationships of Cryptosporidium determined by ribosomal RNA sequence comparison

International Journal for Parasitology ◽

10.1016/0020-7519(90)90093-3 ◽

1990 ◽

Vol 20 (2) ◽

pp. 141-147 ◽

Cited By ~ 34

Author(s):

Alan M. Johnson ◽

Robyn Fielke ◽

Richard Lumb ◽

Peter R. Baverstock

Keyword(s):

Ribosomal Rna ◽

Phylogenetic Relationships ◽

Sequence Comparison ◽

Rna Sequence

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A Transcriptomically Guided Computational Quantification of the SRR8671434 RNA Sequence

10.31219/osf.io/fk6zt ◽

2021 ◽

Author(s):

Rohan Bhansali

Keyword(s):

Data Mining ◽

Rna Sequencing ◽

Ribosomal Rna ◽

Biological Sample ◽

Genomic Library ◽

Rna Sequence ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing ◽

Cellular Transcriptome ◽

Potential Biomarkers

Ribonucleic acid (RNA) is a single strand nucleic acid responsible for genetic coding, decoding, regulation, and expression that consists of phosphate and ribose groups with several purposeful variants, notably messenger, transfer, and ribosomal RNA. RNA sequencing is a next-generation sequencing (NGS) technique capable of continuously analyzing cellular transcriptome and revealing the presence and quantity of RNA within a biological sample. The process entails reverse transcribing the extracted RNA into cDNA; subsequently, the cDNA is fragmented, enabling its input into an NGS workflow, after which adapters are added to both ends of the fragments. RNA sequencing is an expensive and time-consuming process due to its necessitation of an entire genomic library that is often difficult to analyze through traditional methods; however, applying computational methods can overcome these challenges through cutting edge data mining and informatics technologies. In this study, the SRR8671434 RNA sequence is quantified and analyzed for its ability to serve as an indicator for congenital afflictions. The findings in this paper can be applied towards preventing and curing associated diseases, as well as discerning other potential biomarkers within genetic materials.

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