Tomato DNA contains no detectable regions complementary to potato spindle tuber viroid as assayed by solution and filter hybridization

Embryo infection is important for efficient seed transmission of viroids. To identify the major pattern of seed transmission of viroids, we used in situ hybridization to histochemically analyze the distribution of Potato spindle tuber viroid (PSTVd) in each developmental stage of petunia (flowering to mature seed stages). In floral organs, PSTVd was present in the reproductive tissues of infected female × infected male and infected female × healthy male but not of healthy female × infected male before embryogenesis. After pollination, PSTVd was detected in the developed embryo and endosperm in all three crosses. These findings indicate that PSTVd is indirectly delivered to the embryo through ovule or pollen during the development of reproductive tissues before embryogenesis but not directly through maternal tissues as cell-to-cell movement during embryogenesis.

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Characterization of Potato spindle tuber viroid (PSTVd) incidence and new variants from ornamentals

European Journal of Plant Pathology ◽

10.1007/s10658-013-0304-6 ◽

2013 ◽

Vol 138 (1) ◽

pp. 93-101 ◽

Cited By ~ 13

Author(s):

Jaroslav Matoušek ◽

Rajen J. J. Piernikarczyk ◽

Petr Dědič ◽

Josef Mertelík ◽

Kateřina Uhlířová ◽

...

Keyword(s):

Potato Spindle Tuber Viroid

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Alternative Methods of Preparing Whole-Cell DNA from Fungi for Dot-Blot, Restriction Analysis, and Colony Filter Hybridization

Analytical Biochemistry ◽

10.1006/abio.1995.1113 ◽

1995 ◽

Vol 225 (1) ◽

pp. 94-100 ◽

Cited By ~ 11

Author(s):

J. Min ◽

T. Aroanoza ◽

J. Ohrnberger ◽

C. Xu ◽

R.A. Akins

Keyword(s):

Restriction Analysis ◽

Alternative Methods ◽

Dot Blot ◽

Whole Cell ◽

Filter Hybridization

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Potato spindle tuber viroid. [Distribution map].

Distribution Maps of Plant Diseases ◽

10.1079/dmpd/20123172034 ◽

2012 ◽

Author(s):

Keyword(s):

New York ◽

Prince Edward Island ◽

Far East ◽

South Australia ◽

Potato Spindle Tuber Viroid ◽

Persea Americana ◽

Distribution Map ◽

South Wales ◽

Southern Russia ◽

Distribution In Europe

Abstract A new distribution map is provided for Potato spindle tuber viroid. Pospiviroidae: Pospiviroid. Hosts: avocado (Persea americana), tomato (Solanum lycopersicum), potato (S. tuberosum) and other Solanum species. information is given on the geographical distribution in Europe (Austria; Belarus; Belgium; Bulgaria; Croatia; Czech Republic; Finland; France; Germany; Crete, Greece; Ireland; Mainland Italy; Netherlands; Central, Far East, Northern and Southern Russia; Slovenia; Spain; Switzerland; England and Wales, UK; and Ukraine), Asia (Afghanistan; Azerbaijan; Bangladesh; Hebei, Heilongjiang, Jiangsu and Qinghai, China; Georgia; Himachal Pradesh and Maharashtra, India; Iran; Israel; Japan; and Turkey), Africa (Egypt and Nigeria), North America (Alberta, British Columbia, New Brunswick and Prince Edward Island, Canada; Mexico; and Alaska, California, Colorado, Idaho, Maine, Michigan, Minnesota, Mississippi, Montana, Nebraska, New Hampshire, New York, North Dakota, Ohio, Oregon, Washington, Wisconsin and Wyoming), Central America and Caribbean (Costa Rica), South America (Argentina, Chile, Peru and Venezuela) and Oceania (New Zealand, and New South Wales, Northern Territory, South Australia, Western Australia and Victoria, Australia).

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