Oral administration of recombinant salmon growth hormone to rainbow trout, Oncorhynchus mykiss

Aquaculture ◽  
1993 ◽  
Vol 112 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Shunsuke Moriyama ◽  
Hiroshi Yamamoto ◽  
Seiji Sugimoto ◽  
Toshio Abe ◽  
Tetsuya Hirano ◽  
...  
Author(s):  
Brian S. Shepherd ◽  
Jaime K. Johnson ◽  
Jeffrey T. Silverstein ◽  
Ishwar S. Parhar ◽  
Mathilakath M. Vijayan ◽  
...  

2006 ◽  
Vol 49 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Alexandre Ninhaus-Silveira ◽  
Fausto Foresti ◽  
Yara Aiko Tabata ◽  
Marcos Guilherme Rigolino ◽  
Rosicleire Veríssimo-Silveira

Cryopreservation of semen from sex-reversed females of rainbow trout aims at rationalizing the production of stocks composed by 100% females. Semen from normal males (M) and two types of genotypic females (R and G), sex-reversed by the oral administration of 17alpha-methyltestosterone, were used. R was obtained by the fertilization of normal eggs with semen of sex-reversed females while G via gynogenetic reproduction. Semen was diluted in an extender solution (glucose 5,4 g, egg yolk 10 ml, dimetil sulfoxide 10 ml, water 80 ml) at 1:3 ratio (semen/extender), stored in straws of 0.5 ml and freezed in a dry container Cryopac CP-65, at -180ºC. Thawing was performed with water at 70ºC for 3 seconds. There were no significant fertilization rate differences (P>0.05) among thawed semen groups (M = 73.1±11.5%; R = 67.2±23.6%; G = 64±5.8%), confirming that the freezing methodology used was efficient to cryopreserve semen of all three trout groups.


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