Feulgen cytophotometric determination of the DNA content of the embryo proper and suspensor cells of Phaseolus coccineus

1973 ◽  
Vol 2 (2) ◽  
pp. 65-75 ◽  
Author(s):  
Tom Brady
Keyword(s):  
Dna Content ◽  
Phaseolus Coccineus ◽  
Suspensor Cells

Prognostic Implications of Nuclear DNA Content in Head and Neck Cancer

1989 ◽  
Vol 100 (2) ◽  
pp. 95-98 ◽  
Author(s):  
Yang-Chun Guo ◽  
Lawrence Desanto ◽  
Gregory V. Osetinsky
Keyword(s):  
Head And Neck ◽  
Dna Content ◽  
Nuclear Dna ◽  
Clinical Stage ◽  
Nuclear Dna Content ◽  
Survival Advantage ◽  
Prognostic Information ◽  
Diagnosis Of Cancer ◽  
Well Differentiated

The nuclear DNA content was measured in formalin-fixed and deparaffined specimens of 296 oral, pharyngeal, and laryngeal squamous cell carcinomas from patients in whom the clinical outcome was known. One hundred ninety (64%) contained cells with abnormal DNA (DNA aneuploid or tetra/polypoid). Only 32% (60 of 190) of the patients with DNA nondiploid cancers survived 5 years, compared with 49% (52 of 106) of the patients with DNA diploid cancers. When the findings were controlled for clinical stage, patients whose tumors were DNA diploid had a survival advantage at each stage. Histologic grading showed less correlation, because only patients with well-differentiated carcinomas had a survival advantage if their tumors were DNA diploid. These data showed that determination of DNA content in cancers of the head and neck can offer prognostic information not provided by other means and enhance the diagnosis of cancer.

Prognostic Value of Flow Cytometry in Bladder Tumours

1993 ◽  
Vol 60 (2) ◽  
pp. 152-157
Author(s):  
D. Grassi ◽  
M. De Siati ◽  
N. Franzolin
Keyword(s):  
Flow Cytometry ◽  
Transitional Cell Carcinoma ◽  
Cell Carcinoma ◽  
Dna Content ◽  
Prognostic Value ◽  
Transitional Cell ◽  
Prognostic Information ◽  
Carcinoma Of The Bladder ◽  
Bladder Tumours

During this last decade, flow cytometry (FCM) has been widely investigated and employed in assessing the DNA content of bladder tumours. The prognostic value of FCM is recognised by the majority of investigators, above all when it concerns superficial transitional cell carcinoma of the bladder. The determination of ploidy and the degree of aneuploidy seem to offer valuable prognostic information. The Authors have reviewed Literature on this subject, identifying three different categories of studies that analyse the relation of FCM to cytohystological characterisation, to the clinical behaviour of the tumours and to the patients’ survival.

Determination of nuclear DNA content in fungi using mithramycin: vegetative diploidy in Armillaria mellea confirmed

1983 ◽  
Vol 29 (9) ◽  
pp. 1179-1183 ◽  
Author(s):  
A. L. Franklin ◽  
W. G. Filion ◽  
J. B. Anderson
Keyword(s):  
Dna Binding ◽  
Aspergillus Nidulans ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Phytopathogenic Fungus ◽  
Armillaria Mellea ◽  
Dna Measurements

Armillaria mellea, a phytopathogenic fungus, is the only member of the Agaricales (Basidiomycetes) whose fertile vegetative phase in nature is thought to be diploid, rather than dikaryotic. To examine the vegetative ploidy of A. mellea, we used the DNA-binding antibiotic, mithramycin, for fluorometry of in situ nuclear DNA. The measurements of nuclear DNA content indicated that strains derived from single basidiospores of A. mellea were haploid and that strains derived from matings of isolates of single spores were diploid. These data confirm the results of earlier genetic experiments, which show haploidy and diploidy in unmated and mated strains, respectively. Nuclear DNA measurements in known haploid and diploid strains of Aspergillus nidulans confirmed the validity of our protocol.

scholarly journals MICROCHEMICAL DEOXYRIBONUCLEIC ACID DETERMINATION IN INDIVIDUAL CELLS

1961 ◽  
Vol 9 (3) ◽  
pp. 619-626 ◽  
Author(s):  
Jan-Erik Edström ◽  
Jerzy Kawiak
Keyword(s):  
Dna Content ◽  
Mitotic Cycle ◽  
Deoxyribonucleic Acid ◽  
Test Material ◽  
Cell Nuclei ◽  
Fixed Tissue ◽  
Dna Contents ◽  
Two Factors ◽  
Good Agreement

A method for the quantitative determination of DNA in the 50 to 500 µµg. range is presented. Cells or cell nuclei are isolated individually from fixed tissue by means of micromanipulation. The tissue units in question are extracted in an oil chamber with deoxyribonuclease solution. The extracts are evaporated to dryness and redissolved to lens-shaped drops, the DNA contents of which are determined by a photographic-photometric procedure in ultraviolet light. Determinations on calf thymocytes and rat spermatids show a relatively good agreement with biochemical data. The present method tends, however, to give some. what higher values than those reported earlier. The coefficient of variation for analytical values from test material is about ± 10 per cent. The method has been applied to cells from the axolotl, adults as well as tadpoles. Germ cells (spermatids and spermatocytes) do not show any evidence of a biological variation in DNA content. Cells from proliferating tissues give an increased spread of the DNA values. It could be shown, for epithelial cells, that there are at least two factors determining the DNA content of these cells. One is the fact that the cells are investigated at different phases of the mitotic cycle; the other is the fact that the DNA synthesis cycle occupies different ranges for different cells.

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