Effects of long-term elevated serum levels of growth hormone on life expectancy of mice: Lessons from transgenic animal models

1993 ◽  
Vol 68 (1-3) ◽  
pp. 71-87 ◽  
Author(s):  
Eckhard Wolf ◽  
Eva Kahnt ◽  
Jörn Ehrlein ◽  
Walter Hermanns ◽  
Gottfried Brem ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Animal Models ◽  
Life Expectancy ◽  
Elevated Serum ◽  
Serum Levels ◽  
Transgenic Animal ◽  
Transgenic Animal Models

scholarly journals The Cytoskeletal Elements MAP2 and NF-L Show Substantial Alterations in Different Stroke Models While Elevated Serum Levels Highlight Especially MAP2 as a Sensitive Biomarker in Stroke Patients

2021 ◽  
Author(s):  
Bianca Mages ◽  
Thomas Fuhs ◽  
Susanne Aleithe ◽  
Alexandra Blietz ◽  
Constance Hobusch ◽  
...  
Keyword(s):  
Animal Models ◽  
Neuronal Damage ◽  
Degradation Products ◽  
Focal Cerebral Ischemia ◽  
Elevated Serum ◽  
Serum Levels ◽  
Ultrastructural Level ◽  
Stroke Patients ◽  
Protein Levels ◽  
Cytoskeletal Elements

AbstractIn the setting of ischemic stroke, the neurofilament subunit NF-L and the microtubule-associated protein MAP2 have proven to be exceptionally ischemia-sensitive elements of the neuronal cytoskeleton. Since alterations of the cytoskeleton have been linked to the transition from reversible to irreversible tissue damage, the present study investigates underlying time- and region-specific alterations of NF-L and MAP2 in different animal models of focal cerebral ischemia. Although NF-L is increasingly established as a clinical stroke biomarker, MAP2 serum measurements after stroke are still lacking. Therefore, the present study further compares serum levels of MAP2 with NF-L in stroke patients. In the applied animal models, MAP2-related immunofluorescence intensities were decreased in ischemic areas, whereas the abundance of NF-L degradation products accounted for an increase of NF-L-related immunofluorescence intensity. Accordingly, Western blot analyses of ischemic areas revealed decreased protein levels of both MAP2 and NF-L. The cytoskeletal alterations are further reflected at an ultrastructural level as indicated by a significant reduction of detectable neurofilaments in cortical axons of ischemia-affected areas. Moreover, atomic force microscopy measurements confirmed altered mechanical properties as indicated by a decreased elastic strength in ischemia-affected tissue. In addition to the results from the animal models, stroke patients exhibited significantly elevated serum levels of MAP2, which increased with infarct size, whereas serum levels of NF-L did not differ significantly. Thus, MAP2 appears to be a more sensitive stroke biomarker than NF-L, especially for early neuronal damage. This perspective is strengthened by the results from the animal models, showing MAP2-related alterations at earlier time points compared to NF-L. The profound ischemia-induced alterations further qualify both cytoskeletal elements as promising targets for neuroprotective therapies.

scholarly journals Transgenic animal models of tauopathies

2005 ◽  
Vol 1739 (2-3) ◽  
pp. 251-259 ◽  
Author(s):  
Virginia M.-Y. Lee ◽  
Theresa K. Kenyon ◽  
John Q. Trojanowski
Keyword(s):  
Animal Models ◽  
Transgenic Animal ◽  
Transgenic Animal Models

Non-transgenic Animal Models of Alzheimer’s Disease

2017 ◽  
pp. 3-22 ◽  
Author(s):  
Sneha Shree ◽  
Rajat Bhardwaj ◽  
Kashish ◽  
Rahul Deshmukh
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Animal Models ◽  
Transgenic Animal ◽  
Transgenic Animal Models

Early Characterization of Panepinto Micro/Miniature Swine for Use as Transgenic Animal Models

1996 ◽  
pp. 671-679
Author(s):  
Victoria Hampshire ◽  
John Bacher ◽  
Melvin Dennis ◽  
Axel Wolff ◽  
Melissa Yarko
Keyword(s):  
Animal Models ◽  
Transgenic Animal ◽  
Miniature Swine ◽  
Transgenic Animal Models
Sign in / Sign up

Export Citation Format

Share Document