Fine-tuning the topology of a polytopic membrane protein: Role of positively and negatively charged amino acids

Cell ◽  
1990 ◽  
Vol 62 (6) ◽  
pp. 1135-1141 ◽  
Author(s):  
IngMarie Nilsson ◽  
Gunnar von Heijne
Keyword(s):  
Amino Acids ◽  
Membrane Protein ◽  
Fine Tuning ◽  
Charged Amino Acids ◽  
Polytopic Membrane Protein

scholarly journals Energetics of side-chain snorkeling in transmembrane helices probed by nonproteinogenic amino acids

2016 ◽  
Vol 113 (38) ◽  
pp. 10559-10564 ◽  
Author(s):  
Karin Öjemalm ◽  
Takashi Higuchi ◽  
Patricia Lara ◽  
Erik Lindahl ◽  
Hiroaki Suga ◽  
...  
Keyword(s):  
Amino Acids ◽  
Membrane Protein ◽  
Side Chain ◽  
Transmembrane Helices ◽  
Transmembrane Segments ◽  
Charged Amino Acids ◽  
Protein Biogenesis ◽  
Quantitative Basis ◽  
Apparent Free Energy ◽  
Integration Efficiency

Cotranslational translocon-mediated insertion of membrane proteins into the endoplasmic reticulum is a key process in membrane protein biogenesis. Although the mechanism is understood in outline, quantitative data on the energetics of the process is scarce. Here, we have measured the effect on membrane integration efficiency of nonproteinogenic analogs of the positively charged amino acids arginine and lysine incorporated into model transmembrane segments. We provide estimates of the influence on the apparent free energy of membrane integration (ΔGapp) of “snorkeling” of charged amino acids toward the lipid–water interface, and of charge neutralization. We further determine the effect of fluorine atoms and backbone hydrogen bonds (H-bonds) on ΔGapp. These results help establish a quantitative basis for our understanding of membrane protein assembly in eukaryotic cells.

scholarly journals Role of S3 and S4 Transmembrane Domain Charged Amino Acids in Channel Biogenesis and Gating of KCa2.3 and KCa3.1

2008 ◽  
Vol 283 (14) ◽  
pp. 9049-9059 ◽  
Author(s):  
Yajuan Gao ◽  
Cavita K. Chotoo ◽  
Corina M. Balut ◽  
Fei Sun ◽  
Mark A. Bailey ◽  
...  
Keyword(s):  
Amino Acids ◽  
Transmembrane Domain ◽  
Charged Amino Acids

scholarly journals The Role of Positively Charged Amino Acids in ATP Recognition by Human P2X1Receptors

2000 ◽  
Vol 275 (38) ◽  
pp. 29361-29367 ◽  
Author(s):  
Steven Ennion ◽  
Sarah Hagan ◽  
Richard J. Evans
Keyword(s):  
Amino Acids ◽  
Charged Amino Acids ◽  
Positively Charged

scholarly journals Role of Positively Charged Amino Acids in the M2DTransmembrane Helix of Ktr/Trk/HKT Type Cation Transporters

Channels ◽  
2007 ◽  
Vol 1 (3) ◽  
pp. 161-171 ◽  
Author(s):  
Naoki Kato ◽  
Masaro Akai ◽  
Lalu Zulkifli ◽  
Nobuyuki Matsuda ◽  
Yasuhiro Kato ◽  
...  
Keyword(s):  
Amino Acids ◽  
Charged Amino Acids ◽  
Cation Transporters ◽  
Positively Charged

scholarly journals Role of negatively charged amino acids in β4 F-loop in activation and desensitization of α3β4 rat neuronal nicotinic receptors

2008 ◽  
Vol 1778 (4) ◽  
pp. 864-871 ◽  
Author(s):  
Jiří Lindovský ◽  
Martina Kaniaková ◽  
Lucie Svobodová ◽  
František Vyskočil ◽  
Jan Krůšek
Keyword(s):  
Amino Acids ◽  
Nicotinic Receptors ◽  
Neuronal Nicotinic Receptors ◽  
Charged Amino Acids
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