A simple protocol for successful bone marrow chromosome studies dispatched over great distances

1988 ◽  
Vol 32 (2) ◽  
pp. 299 ◽  
Author(s):  
A. Daniel ◽  
L. Ekblom ◽  
S. Phillips
Keyword(s):  
Bone Marrow ◽  
Simple Protocol ◽  
Bone Marrow Chromosome

scholarly journals Longitudinal bone marrow chromosome studies in potential leukemic myeloid disorders

Cancer ◽  
1973 ◽  
Vol 31 (3) ◽  
pp. 509-515 ◽  
Author(s):  
Rubén Lisker ◽  
Azyadéh Cobo De Gutiérrez ◽  
Miguel Velázquez-Ferrari
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Chromosome

Direct and Repeatable Bone Marrow Chromosome Preparations from Living Mice

1971 ◽  
Vol 46 (6) ◽  
pp. 285-288 ◽  
Author(s):  
Mary J. Seller ◽  
Armando A. Mendes
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Chromosome

scholarly journals Clastogenic Effect of Carthamus lanatus L. (Asteraceae)

2003 ◽  
Vol 58 (11-12) ◽  
pp. 833-836
Author(s):  
Margarita Topashka-Ancheva ◽  
Rilka Taskova ◽  
Nedjalka Handjieva ◽  
Bozhanka Mikhova ◽  
Helmut Duddeck
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Chromosome Aberrations ◽  
Water Extract ◽  
Positive Control ◽  
Aerial Parts ◽  
Chromosome Aberration Assay ◽  
Different Types ◽  
Clastogenic Effect ◽  
Bone Marrow Chromosome

Abstract The clastogenic effect of total dichloromethane, methanol and water extracts, four bioactive fractions and three individual constituents from Carthamus lanatus aerial parts were evaluated in mice by bone marrow chromosome aberration assay with mitomycin C as positive control. Significant differences in the percentage of aberrant mitosis of the extracts were observed. The dichloromethane extract exhibited a considerable clastogenic effect and the water extract a negligible one. Different types of chromosome aberrations and time-dependant effects for the active fractions and individual compounds were found.

scholarly journals Minced Umbilical Cord Fragments as a Source of Cells for Orthopaedic Tissue Engineering: An In Vitro Study

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
A. Marmotti ◽  
S. Mattia ◽  
M. Bruzzone ◽  
S. Buttiglieri ◽  
A. Risso ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Stem Cell ◽  
Umbilical Cord ◽  
In Vitro Study ◽  
Enzymatic Digestion ◽  
Mean Value ◽  
Simple Protocol ◽  
Multilineage Potential

A promising approach for musculoskeletal repair and regeneration is mesenchymal-stem-cell- (MSC-)based tissue engineering. The aim of the study was to apply a simple protocol based on mincing the umbilical cord (UC), without removing any blood vessels or using any enzymatic digestion, to rapidly obtain an adequate number of multipotent UC-MSCs. We obtained, at passage 1 (P1), a mean value of4,2×106cells (SD 0,4) from each UC. At immunophenotypic characterization, cells were positive for CD73, CD90, CD105, CD44, CD29, and HLA-I and negative for CD34 and HLA-class II, with a subpopulation negative for both HLA-I and HLA-II. Newborn origin and multilineage potential toward bone, fat, cartilage, and muscle was demonstrated. Telomere length was similar to that of bone-marrow (BM) MSCs from young donors. The results suggest that simply collecting UC-MSCs at P1 from minced umbilical cord fragments allows to achieve a valuable population of cells suitable for orthopaedic tissue engineering.

When are apparently non-clonal abnormalities in bone marrow chromosome studies actually clonal?

2012 ◽  
Vol 205 (7-8) ◽  
pp. 405-409 ◽  
Author(s):  
Chandra Hutchens ◽  
Rhett P. Ketterling ◽  
Daniel L. Van Dyke
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Chromosome ◽  
Clonal Abnormalities

A direct bone marrow chromosome technique for acute lymphoblastic leukemia

1984 ◽  
Vol 13 (3) ◽  
pp. 239-257 ◽  
Author(s):  
Dorothy L. Williams ◽  
Ann Harris ◽  
Kenna J. Williams ◽  
Marie Joan Brosius ◽  
Wanda Lemonds
Keyword(s):  
Bone Marrow ◽  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Bone Marrow Chromosome
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