The bacterial flora of non-carbonated, natural mineral water from the springs to reservoir and glass and plastic bottles

1990 ◽  
Vol 11 (1) ◽  
pp. 51-71 ◽  
Author(s):  
Thomas Bischofberger ◽  
S.K. Cha ◽  
R. Schmitt ◽  
Bärbel König ◽  
W. Schmift-Lorenz
Keyword(s):  
Mineral Water ◽  
Bacterial Flora ◽  
Natural Mineral ◽  
Natural Mineral Water

scholarly journals Diversity of Bacteria Growing in Natural Mineral Water after Bottling

2005 ◽  
Vol 71 (7) ◽  
pp. 3624-3632 ◽  
Author(s):  
Alexander Loy ◽  
Wolfgang Beisker ◽  
Harald Meier
Keyword(s):  
Bacterial Community ◽  
Bacterial Growth ◽  
Mineral Water ◽  
Bacterial Community Composition ◽  
Bacterial Flora ◽  
Significant Proportion ◽  
Mineral Waters ◽  
Rrna Gene ◽  
Natural Mineral ◽  
Natural Mineral Water

ABSTRACT Bacterial growth occurs in noncarbonated natural mineral waters a few days after filling and storage at room temperature, a phenomenon known for more than 40 years. Using the full-cycle rRNA approach, we monitored the development of the planktonic bacterial community in a noncarbonated natural mineral water after bottling. Seven 16S rRNA gene libraries, comprising 108 clones in total, were constructed from water samples taken at various days after bottling and from two different bottle sizes. Sequence analyses identified 11 operational taxonomic units (OTUs), all but one affiliated with the betaproteobacterial order Burkholderiales (6 OTUs) or the class Alphaproteobacteria (4 OTUs). Fluorescence in situ hybridization (FISH) was applied in combination with DAPI (4′,6′-diamidino-2-phenylindole) staining, viability staining, and microscopic counting to quantitatively monitor changes in bacterial community composition. A growth curve similar to that of a bacterium grown in a batch culture was recorded. In contrast to the current perception that Gammaproteobacteria are the most important bacterial components of natural mineral water in bottles, Betaproteobacteria dominated the growing bacterial community and accounted for 80 to 98% of all bacteria detected by FISH in the late-exponential and stationary-growth phases. Using previously published and newly designed genus-specific probes, members of the betaproteobacterial genera Hydrogenophaga, Aquabacterium, and Polaromonas were found to constitute a significant proportion of the bacterial flora (21 to 86% of all bacteria detected by FISH). For the first time, key genera responsible for bacterial growth in a natural mineral water were identified by applying molecular cultivation-independent techniques.

scholarly journals THE BACTERIAL FLORA IN BOTTLED NATURAL MINERAL WATER SOLD IN ITALY

1998 ◽  
Vol 21 (3) ◽  
pp. 175-185 ◽  
Author(s):  
S. MASSA ◽  
M. FANELLI ◽  
M.T. BRIENZA ◽  
M. SINIGAGLIA
Keyword(s):  
Mineral Water ◽  
Bacterial Flora ◽  
Natural Mineral ◽  
Natural Mineral Water

Aava Natural Mineral Water (A)

2015 ◽  
Author(s):  
Anand Jaiswal ◽  
Harit Palan ◽  
Ingita Jain
Keyword(s):  
Mineral Water ◽  
Natural Mineral ◽  
Natural Mineral Water

scholarly journals Can cyanobacteria infect underground water sources? Evidence from a small scale monitoring of natural mineral water drinking source

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Spyros Gkelis ◽  
Aristidis Vlamis
Keyword(s):  
16S Rdna ◽  
Mineral Water ◽  
Monitoring Program ◽  
Water Source ◽  
Biosynthetic Gene Cluster ◽  
Underground Water ◽  
Cyanobacterial Blooms ◽  
Small Scale ◽  
Natural Mineral ◽  
Natural Mineral Water

The expansion of harmful cyanobacterial blooms is of worldwide concern as they have increased globally in frequency and intensity in recent decades. A cyanobacterial colony was found in a bottle of natural mineral water of a small water company in July 2012, which led to a further examination for a period of five months (July-November 2012) of both the bottled filtered water and the originating groundwater source (N. Greece) for the occurrence of Cyanobacteria. Cyanobacteria occurrence was monitored by microscopy and cyanospecific 16S rDNA amplification; potentially toxic species occurrence was screened by mcyA gene (known to take part in the MC-biosynthetic gene cluster) amplification. The highest abundance of cyanobacterial cells without the simultaneous presence of the mcyA gene, was measured in July, in contrast to October when the presence of cyanobacteria was only identified by tracing cyanospecific 16S rDNA and the mcyA gene region in the underground water source. The results of this small scale monitoring program indicate the potential existence of an emerging danger for human health in a relatively manageable product such as the bottled natural mineral water. 

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