The use of isolated rat hepatocytes to measure unscheduled DNA synthesis as a screen for chemical carcinogens

Toxicology ◽  
1980 ◽  
Vol 18 (3) ◽  
pp. 233-237 ◽  
Author(s):  
Carl N. Martin
Keyword(s):  
Dna Synthesis ◽  
Rat Hepatocytes ◽  
Unscheduled Dna Synthesis ◽  
Chemical Carcinogens ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

scholarly journals Fluorescence-activated sorting of rat hepatocytes based on their mixed function oxidase activities towards diethoxyfluorescein

1987 ◽  
Vol 247 (1) ◽  
pp. 23-28 ◽  
Author(s):  
I N H White ◽  
M L Green ◽  
R F Legg
Keyword(s):  
Dna Synthesis ◽  
Trypan Blue ◽  
Rat Hepatocytes ◽  
Fold Increase ◽  
Mixed Function Oxidase ◽  
Unscheduled Dna Synthesis ◽  
Rich Region ◽  
Trypan Blue Exclusion ◽  
Isolated Rat Hepatocytes ◽  
Viable Cells

The formation of ethoxyfluorescein and fluorescein from diethoxyfluorescein by isolated rat hepatocytes has been used as a basis for separating such cells dependent on their mixed function oxidase activities by fluorescence-activated flow cytometry. Five equal fractions defined by computer-generated regions were isolated. Non-viable cells with low fluorescence (region 1) represented 10-15% of the population, while the remainder with higher mixed function oxidase activities (regions 2-5), were greater than 95% viable by Trypan Blue exclusion. In region 1, 30% of the viable cells were binucleate, 67% diploid while in region 5, 13% were binucleate and 69% tetraploid. At 3 h after sorting, following attachment to glass coverslips, exposure of cells to methyl methanesulphonate, retrorsine or norethindrone resulted in unscheduled DNA synthesis which was 2-fold higher in the tetraploid-rich region 5, while aflatoxin B1, benzo[a]pyrene or 2-acetylaminofluorene caused a 5-fold increase in unscheduled DNA synthesis in these cells, relative to the diploid-rich hepatocytes in region 2.

scholarly journals Lysosomal triacylglycerol lipase and lipolysis in isolated rat hepatocytes.

1979 ◽  
Vol 254 (18) ◽  
pp. 8841-8846
Author(s):  
L.J. Debeer ◽  
J. Thomas ◽  
P.J. De Schepper ◽  
G.P. Mannaerts
Keyword(s):  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Triacylglycerol Lipase ◽  
Isolated Rat
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