Brain-derived neurotrophic factor, but not neurotrophin-3, prevents ischaemia-induced neuronal cell death in organotypic rat hippocampal slice cultures

1996 ◽  
Vol 211 (3) ◽  
pp. 203-206 ◽  
Author(s):  
A.K. Pringle ◽  
L.E. Sundstrom ◽  
G.J.C. Wilde ◽  
L.R. Williams ◽  
F. lannotti
Keyword(s):  
Cell Death ◽  
Neurotrophic Factor ◽  
Hippocampal Slice ◽  
Neuronal Cell Death ◽  
Neuronal Cell ◽  
Brain Derived Neurotrophic Factor ◽  
Hippocampal Slice Cultures ◽  
Neurotrophin 3

scholarly journals Na+, K+-ATPase inhibition induces neuronal cell death in rat hippocampal slice cultures: Association with GLAST and glial cell abnormalities

2018 ◽  
Vol 138 (3) ◽  
pp. 167-175 ◽  
Author(s):  
Yuki Kurauchi ◽  
Kazuki Noma ◽  
Akinori Hisatsune ◽  
Takahiro Seki ◽  
Hiroshi Katsuki
Keyword(s):  
Cell Death ◽  
Glial Cell ◽  
Hippocampal Slice ◽  
Neuronal Cell Death ◽  
Neuronal Cell ◽  
Hippocampal Slice Cultures ◽  
Atpase Inhibition

scholarly journals Astrocytes Protect against Isoflurane Neurotoxicity by Buffering pro-brain–derived Neurotrophic Factor

2015 ◽  
Vol 123 (4) ◽  
pp. 810-819 ◽  
Author(s):  
Creed M. Stary ◽  
Xiaoyun Sun ◽  
Rona G. Giffard
Keyword(s):  
Cell Death ◽  
Neurotrophic Factor ◽  
Neuronal Cell Death ◽  
Neuronal Cell ◽  
Brain Derived Neurotrophic Factor ◽  
Neurotrophin Receptor ◽  
Enzyme Linked Immunosorbent Assay ◽  
Neuronal Cultures ◽  
P75 Neurotrophin Receptor ◽  
Primary Neuronal Cultures

Abstract Background: Isoflurane induces cell death in neurons undergoing synaptogenesis via increased production of pro-brain–derived neurotrophic factor (proBDNF) and activation of postsynaptic p75 neurotrophin receptor (p75NTR). Astrocytes express p75NTR, but their role in neuronal p75NTR-mediated cell death remains unclear. The authors investigated whether astrocytes have the capacity to buffer increases in proBDNF and protect against isoflurane/p75NTR neurotoxicity. Methods: Cell death was assessed in day in vitro (DIV) 7 mouse primary neuronal cultures alone or in co-culture with age-matched or DIV 21 astrocytes with propidium iodide 24 h after 1 h exposure to 2% isoflurane or recombinant proBDNF. Astrocyte-targeted knockdown of p75NTR in co-culture was achieved with small-interfering RNA and astrocyte-specific transfection reagent and verified with immunofluorescence microscopy. proBDNF levels were assessed by enzyme-linked immunosorbent assay. Each experiment used six to eight replicate cultures/condition and was repeated at least three times. Results: Exposure to isoflurane significantly (P < 0.05) increased neuronal cell death in primary neuronal cultures (1.5 ± 0.7 fold, mean ± SD) but not in co-culture with DIV 7 (1.0 ± 0.5 fold) or DIV 21 astrocytes (1.2 ± 1.2 fold). Exogenous proBDNF dose dependently induced neuronal cell death in both primary neuronal and co-cultures, an effect enhanced by astrocyte p75NTR inhibition. Astrocyte-targeted p75NTR knockdown in co-cultures increased media proBDNF (1.2 ± 0.1 fold) and augmented isoflurane-induced neuronal cell death (3.8 ± 3.1 fold). Conclusions: The presence of astrocytes provides protection to growing neurons by buffering increased levels of proBDNF induced by isoflurane. These findings may hold clinical significance for the neonatal and injured brain where increased levels of proBDNF impair neurogenesis.

Brain-derived neurotrophic factor prevents neuronal cell death induced by corticosterone

1999 ◽  
Vol 57 (2) ◽  
pp. 227-235 ◽  
Author(s):  
Atsumi Nitta ◽  
Makoto Ohmiya ◽  
Ayako Sometani ◽  
Megumi Itoh ◽  
Hiroshi Nomoto ◽  
...  
Keyword(s):  
Cell Death ◽  
Neurotrophic Factor ◽  
Neuronal Cell Death ◽  
Neuronal Cell ◽  
Brain Derived Neurotrophic Factor
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