Further studies on dopamine and during the pupal stage of Papilio xuthus (Lepidoptera: Papilionidae)

Author(s):  
Yumi Ishizaki ◽  
Yoshishige Umebachi
Keyword(s):  
Author(s):  
Eric Hallberg ◽  
Lina Hansén

The antennal rudiments in lepidopterous insects are present as disks during the larval stage. The tubular double-walled antennal disk is present beneath the larval antenna, and its inner layer gives rise to the adult antenna during the pupal stage. The sensilla develop from a cluster of cells that are derived from one stem cell, which gives rise to both sensory and enveloping cells. During the morphogenesis of the sensillum these cells undergo major transformations, including cell death. In the moth Agrotis segetum the pupal stage lasts about 14 days (temperature, 25°C). The antennae, clearly seen from the exterior, were dissected and fixed according to standard procedures (3 % glutaraldehyde in 0.15 M cacaodylate buffer, followed by 1 % osmiumtetroxide in the same buffer). Pupae from day 1 to day 8, of both sexes were studied.


Genetics ◽  
1998 ◽  
Vol 149 (3) ◽  
pp. 1465-1475 ◽  
Author(s):  
T Kozlova ◽  
G V Pokholkova ◽  
G Tzertzinis ◽  
J D Sutherland ◽  
I F Zhimulev ◽  
...  

Abstract DHR38 is a member of the steroid receptor superfamily in Drosophila homologous to the vertebrate NGFI-B-type orphan receptors. In addition to binding to specific response elements as a monomer, DHR38 interacts with the USP component of the ecdysone receptor complex in vitro, in yeast and in a cell line, suggesting that DHR38 might modulate ecdysone-triggered signals in the fly. We characterized the molecular structure and expression of the Dhr38 gene and initiated an in vivo analysis of its function(s) in development. The Dhr38 transcription unit spans more than 40 kb in length, includes four introns, and produces at least four mRNA isoforms differentially expressed in development; two of these are greatly enriched in the pupal stage and encode nested polypeptides. We characterized four alleles of Dhr38: a P-element enchancer trap line, l(2)02306, which shows exclusively epidermal staining in the late larval, pre-pupal and pupal stages, and three EMS-induced alleles. Dhr38 alleles cause localized fragility and rupturing of the adult cuticle, demonstrating that Dhr38 plays an important role in late stages of epidermal metamorphosis.


Insects ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 42
Author(s):  
Samy Sayed ◽  
Sayed-Ashraf Elarrnaouty ◽  
Saad AlOtaibi ◽  
Mohamed Salah

This study aimed to estimate the virulence of an indigenous Beauveria bassiana on all developmental stages of two indigenous coccinellids; Coccinella undecimpunctata and Hippodamia variegata through three application methods; direct spray, contact method, and feeding on aphids treated with the fungus (ingestion). Also, indirect effect on all developmental stages resulted from 1st larval instar treated with these application methods. All treatments were done with a concentration of 1 × 105 which was recommended in previous studies for different aphid species with a control of 0.02% Tween 80 (v/v). The mortality of 1st larval instar of both H. variegata and C. undecimpunctata and pupal stage of C. undecimpunctata were significantly increased with spray method only. Also, contact method achieved significantly higher mortality on 1st larval instar of C. undecimpunctata only. Regard to indirect effect, except of mortality of 1st larval instar of both predators and 2nd larval instar of H. variegata, other developmental instars/stages of both predators were not affected by B. bassiana through the three tested application methods in the mortality, duration, survival, cumulative survival male and female longevity, and fecundity. Therefore, both tested predatory coccinellids could be compatible with this indigenous isolate of B. bassiana where, in general, there are no negative effects of the fungus on both predators.


1955 ◽  
Vol 87 (6) ◽  
pp. 275-276
Author(s):  
D. A. Ross

Difficulties experienced in rearing Feralia spp. beyond the pupal stage, and inability to distinguish species in the immature forms, has led to a study of their larvae and pupae. So far no specific differences have been observed in the larvae but progress has been made in the identification of Feralia in the pupal stage.


1914 ◽  
Vol 21 (6) ◽  
pp. 190-192 ◽  
Author(s):  
H. S. Barber
Keyword(s):  

1966 ◽  
Vol 44 (12) ◽  
pp. 1571-1575 ◽  
Author(s):  
K. D. Chaudhary ◽  
A. Lemonde

The in vivo synthesis of deoxyribonucleic acid (DNA), as shown by the rate of incorporation of14C-thymidine, has been investigated at different stages in the life cycle of Tribolium confusum. During the larval period, a close similarity is observed between the rate of DNA synthesis and the pattern of growth. The pupal stage, which is a non-growth phase, is characterized by a cessation of DNA synthesis. During the larval growth phase, although the 3-day-old larvae have the lowest and the 13-day-old have the highest rate of DNA synthesis, the rate of DNA degradation in the older larvae is almost twice as great as that of the younger larvae. These findings are consistent with the observed total concentration of DNA of the insect at these stages.


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