Molecular cloning and heterologous expression of a Klebsiella pneumoniae gene encoding alginate lyase

Gene ◽  
1989 ◽  
Vol 75 (1) ◽  
pp. 127-134 ◽  
Author(s):  
R.C. Caswell ◽  
P. Gacesa ◽  
K.E. Lutrell ◽  
A.J. Weightman
Keyword(s):  
Klebsiella Pneumoniae ◽  
Heterologous Expression ◽  
Molecular Cloning ◽  
Alginate Lyase ◽  
Gene Encoding

Molecular Cloning and Expression of β-1,4-endoxylanase Gene from Chaetomium cupreum in Yeast Saccharomyces cerevisiae

2013 ◽  
Vol 779-780 ◽  
pp. 191-194
Author(s):  
Hai Yan Zhang ◽  
Wen Rao Li ◽  
Min Li
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleotide Sequence ◽  
Heterologous Expression ◽  
Molecular Mass ◽  
Molecular Cloning ◽  
Cloning And Expression ◽  
Cdna Fragment ◽  
Gene Encoding ◽  
Calculated Molecular Mass

The gene encoding an endo-β-1,4-xylanase (XynCC) fromchaetomium cupreumwas amplified using PCR. The nucleotide sequence of a 690 bp cDNA fragment was determined. Based on the nucleotide sequence, calculated molecular mass of the enzyme was 24.7 kDa. The XynCC gene was inserted into the pYES2 vector and transferred into the cells ofS. cerevisiaeH158 for heterologous expression.

scholarly journals Molecular Cloning and Heterologous Expression of the Gene Encoding Dihydrogeodin Oxidase, a Multicopper Blue Enzyme fromAspergillus terreus

1995 ◽  
Vol 270 (37) ◽  
pp. 21495-21502 ◽  
Author(s):  
Ke-xue Huang ◽  
Isao Fujii ◽  
Yutaka Ebizuka ◽  
Katsuya Gomi ◽  
Ushio Sankawa
Keyword(s):  
Heterologous Expression ◽  
Molecular Cloning ◽  
Gene Encoding

Cloning, sequencing and heterologous expression of a Klebsiella pneumoniae gene encoding an FAD-independent acetolactate synthase

Gene ◽  
1992 ◽  
Vol 117 (1) ◽  
pp. 125-130 ◽  
Author(s):  
Hwei-Ling Peng ◽  
Pei-Yu Wang ◽  
Chiou-Mei Wu ◽  
Der-Chian Hwang ◽  
Hwan-You Chang
Keyword(s):  
Klebsiella Pneumoniae ◽  
Heterologous Expression ◽  
Acetolactate Synthase ◽  
Gene Encoding
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