Cytochrome P450-mediated activation of phenanthrene in genetically engineered V79 Chinese hamster cells

1996 ◽  
Vol 1 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Jürgen Jacob ◽  
Gottfried Raab ◽  
Volker Soballa ◽  
Wolfgang A. Schmalix ◽  
Gernot Grimmer ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Chinese Hamster ◽  
Genetically Engineered ◽  
Chinese Hamster Cells

Cytochrome P450 mediated reactions studied in genetically engineered V79 Chinese hamster cells

1995 ◽  
Vol 5 (Special Issue) ◽  
pp. S91-S96 ◽  
Author(s):  
Johannes Doehmer ◽  
Dietrich Holtkamp ◽  
Volker Soballa ◽  
Gottfried Raab ◽  
Wolfgang Schmalix ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Chinese Hamster ◽  
Genetically Engineered ◽  
Chinese Hamster Cells

Stable expression of human cytochrome P450 2E1 in V79 Chinese hamster cells

1995 ◽  
Vol 293 (2) ◽  
pp. 123-131 ◽  
Author(s):  
Wolfgang A. Schmalix ◽  
Martina Barrenscheen ◽  
Robert Landsiedel ◽  
Christine Janzowski ◽  
Gerhard Eisenbrand ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Chinese Hamster ◽  
Stable Expression ◽  
Cytochrome P450 2E1 ◽  
Chinese Hamster Cells ◽  
Human Cytochrome

Bioactivation and Toxicity of Two Anthraquinones in V79 Chinese Hamster Cells

1991 ◽  
Vol 19 (1) ◽  
pp. 48-52
Author(s):  
Maurizio Mian ◽  
Duccio Fratta ◽  
Giuseppe Rainaldi ◽  
Dino Benetti ◽  
Pier Giovanni Gervasi
Keyword(s):  
Cytochrome P450 ◽  
Nadh Dehydrogenase ◽  
Chinese Hamster ◽  
Cytochrome P450 Reductase ◽  
V79 Cells ◽  
Chinese Hamster Cells ◽  
P450 Reductase ◽  
Nadph Cytochrome P450 Reductase ◽  
Dt Diaphorase

The role of the flavoprotein NADPH cytochrome P450 reductase, NADH-dehydrogenase and DT-diaphorase in the biotransformation of the anthraquinones rhein and danthron was investigated using purified enzymes, subcellular fractions and cell cultures. The results demonstrated that rhein was more able than danthron to produce O2 by P450 reductase, NADH-dehydrogenase and V79 homogenate and microsomes, and this may explain its higher toxicity to V79 cells. In addition, unlike other quinones, DT-diaphorase appeared not to play a detoxication role in rhein and danthron biotransformation. On the contrary, by inhibiting DT-diaphorase with dicoumarol, the rhein- and danthron-derived cytotoxicity in V79 cells was reduced. This finding suggests that, for these quinones, other coumarol-sensitive biotransformation routes are operative in V79 cells.

Genetically engineered V79 Chinese hamster cells for stable expression of human cytochrome P450IA2

1992 ◽  
Vol 228 (2-3) ◽  
pp. 95-102 ◽  
Author(s):  
Catherine Wölfel ◽  
Barbara Heinrich-Hirsch ◽  
Thomas Schulz-Schalge ◽  
Albrecht Seidel ◽  
Heinz Frank ◽  
...  
Keyword(s):  
Chinese Hamster ◽  
Genetically Engineered ◽  
Stable Expression ◽  
Chinese Hamster Cells ◽  
Human Cytochrome
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