THE DEVELOPMENT OF ENDOCYTOTIC ABILITY IN THE FOLLICLE-ASSOCIATED EPITHELIUM OF THE BURSA OF FABRICIUS

AbstractThe aim of this study was to determine whether dietary supplementation with zinc oxide nanoparticales (NP-ZnO) as a substitute for the conventional ZnO affects the intestinal digestibility of selected minerals, growth performance and meat quality in turkeys. The replacement of ZnO with NP-ZnO had no effect on the intestinal digestibility of Zn, Cu, Fe and Ca, whereas the lowest dose of supplemental Zn reduced Zn digestibility. The applied inclusion levels and sources of Zn had no effect on the growth performance (except the feed intake) of turkeys, including liveability. No differences in the relative weights of the heart, spleen and bursa of Fabricius (except the liver), or the weights of the femur and tibia were found between the dietary treatments. Neither the dose nor the source of supplemental Zn influenced carcass dressing percentage or the share of breast, thigh and drumstick muscles in the carcass. In comparison with the highest and moderate doses of Zn, the lowest inclusion level of Zn contributed to increased yellowness of breast meat (P=0.005). The analyzed doses and sources of supplemental Zn exerted varied effects on the redox status of fresh and frozen breast meat. In conclusion, the growth performance of turkeys, carcass yield and composition as well as the redox status of fresh and frozen breast meat were generally similar, regardless of the dietary source and level of Zn. The beneficial effect of Zn addition at 100 mg/kg was improved Zn and Ca digestibility, and increased redness of breast meat.

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Immunomodulatory Effect of Andrographis paniculata during Lead Induced Toxicity in Broilers

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.v12i2.3742 ◽

2016 ◽

Vol 12 (2) ◽

Author(s):

R. S. Ingole ◽

A. S. Chandewar ◽

M. V. Joshi ◽

Madhuri Hedau ◽

S W Hajare ◽

...

Keyword(s):

Lead Acetate ◽

Lead Toxicity ◽

Andrographis Paniculata ◽

Immunomodulatory Effect ◽

Bursa Of Fabricius ◽

Control Group ◽

Skin Thickness ◽

Broiler Chicks ◽

Commercial Diet ◽

Ameliorative Effect

The effect of dietary lead exposure on immunomodulation of broilers and possible ameliorative effect of Andrographis paniculata were studied. One hundred broiler chicks were randomly divided into five equal groups of 20 each. Group T0 served as control while group T1, T2, T3 and T4 birds were provided commercial diet with lead acetate at the dose of 300 ppm in feed. Powder of dry leaves of Andrographis paniculata was mixed in the feed of group T2, T3 and T4 chicks at the dose of 2 gm, 3 gm and 4 gm/kg of feed respectively and fed for 4 weeks. Significant decrease in HI titres in T1 group and significant increase in T4 group suggested increased haemagglutination titre (HI) response. The maximum skin thickness and diameter on 24th and 48th hours was observed in control group birds (T0) and was comparable with T4 group. Microscopically spleen, thymus and bursa of fabricius revealed increase in lymphoid population in birds fed lead acetate along with Andrographis paniculata compared to T1 group. We concluded that Andrographis paniculata has immunomodulatory effect during lead toxicity in broilers.

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Pax-5 and EBF are Expressed in Committed B-Cell Progenitors Prior to the Colonization of the Embryonic Bursa of Fabricius*. FRONTLINES

Scandinavian Journal of Immunology ◽

10.1046/j.1365-3083.2000.00821.x ◽

2000 ◽

Vol 52 (5) ◽

pp. 465-469 ◽

Cited By ~ 8

Author(s):

P. Nieminen ◽

J. Liippo ◽

O. Lassila

Keyword(s):

B Cell ◽

Bursa Of Fabricius

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Antagonism of Adherent Invasive E. coli LF82 With Human α-defensin 5 in the Follicle-associated Epithelium of Patients With Ileal Crohn’s Disease

Inflammatory Bowel Diseases ◽

10.1093/ibd/izaa315 ◽

2020 ◽

Author(s):

Lina Y Alkaissi ◽

Martin E Winberg ◽

Stéphanie DS Heil ◽

Staffan Haapaniemi ◽

Pär Myrelid ◽

...

Keyword(s):

Crohn’S Disease ◽

Crohn's Disease ◽

Ex Vivo ◽

Ussing Chambers ◽

E Coli ◽

Follicle Associated Epithelium ◽

Vitro Model ◽

Set Up

Abstract Background The first visible signs of Crohn’s disease (CD) are microscopic erosions over the follicle-associated epithelium (FAE). The aim of the study was to investigate the effects of human α-defensin 5 (HD5) on adherent-invasive Escherichia coli LF82 translocation and HD5 secretion after LF82 exposure in an in vitro model of human FAE and in human FAE ex vivo. Methods An in vitro FAE-model was set up by the coculture of Raji B cells and Caco-2-cl1 cells. Ileal FAE from patients with CD and controls were mounted in Ussing chambers. The effect of HD5 on LF82 translocation was studied by LF82 exposure to the cells or tissues with or without incubation with HD5. The HD5 secretion was measured in human FAE exposed to LF82 or Salmonella typhimurium. The HD5 levels were evaluated by immunofluorescence, immunoblotting, and ELISA. Results There was an increased LF82 translocation across the FAE-model compared with Caco-2-cl1 (P < 0.05). Incubation of cell/tissues with HD5 before LF82 exposure reduced bacterial passage in both models. Human FAE showed increased LF82 translocation in CD compared with controls and attenuated passage after incubation with sublethal HD5 in both CD and controls (P < 0.05). LF82 exposure resulted in a lower HD5 secretion in CD FAE compared with controls (P < 0.05), whereas Salmonella exposure caused equal secretion on CD and controls. There were significantly lower HD5 levels in CD tissues compared with controls. Conclusions Sublethal HD5 reduces the ability of LF82 to translocate through FAE. The HD5 is secreted less in CD in response to LF82, despite a normal response to Salmonella. This further implicates the integrated role of antimicrobial factors and barrier function in CD pathogenesis.

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