OESTRUS CONTROL AND EARLY PREGNANCY DIAGNOSIS IN THE SWAMP BUFFALO: COMPARISON OF ENZYME IMMUNOASSAY AND RADIOIMMUNOASSAY FOR PLASMA PROGESTERONE

1981 ◽  
pp. 141-147
Author(s):  
Maneewan Kamonpatana ◽  
Dick F.M. van de Wiel ◽  
Wim Koops ◽  
Dumrong Leenanuruksa ◽  
Chunpen Ngramsuriyaroj ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Early Pregnancy ◽  
Plasma Progesterone ◽  
Pregnancy Diagnosis ◽  
Swamp Buffalo

Oestrus control and early pregnancy diagnosis in the swamp buffalo: Comparison of enzymeimmunoassay and radioummunoassay for plasma progesterone

1979 ◽  
Vol 11 (5) ◽  
pp. 399-409 ◽  
Author(s):  
Maneewan Kamonpatana ◽  
Dick F.M. van de Wiel ◽  
Wim Koops ◽  
Dumrong Leenanuruksa ◽  
Chunpen Ngramsuriyaroj ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Plasma Progesterone ◽  
Pregnancy Diagnosis ◽  
Swamp Buffalo

314 EARLY PREGNANCY DIAGNOSIS USING A NOVEL TRANSRECTAL ULTRASONOGRAPHY PROTOCOL IN JAPANESE BLACK COWS

2009 ◽  
Vol 21 (1) ◽  
pp. 254
Author(s):  
A. Gaja ◽  
C. Kubota ◽  
T. Kojima
Keyword(s):  
Corpus Luteum ◽  
Early Pregnancy ◽  
Transrectal Ultrasonography ◽  
Blood Collection ◽  
Sectional Area ◽  
Cross Sectional ◽  
Plasma Progesterone ◽  
Area Change ◽  
Pregnancy Diagnosis ◽  
Peripheral Plasma

The present study aims to establish a novel practical protocol for early pregnancy diagnosis in cows by using transrectal ultrasonography. The protocol is based on measurements of corpus luteum (CL) cross-sectional area (CL c-s area) change performed at 2 separate days before the coming estrus after AI. Fourteen cows were inseminated artificially, and transrectal ultrasonographical observation of the ovaries and blood collection for measurement of peripheral plasma progesterone (P4) concentration were carried out daily from Days 12 to 23 (Day 0 = the day of onset of estrus). Thereafter, cows were routinely diagnosed for pregnancy at Day 30 by transrectal ultrasonography. The largest CL c-s area was obtained at Day 14 in both pregnant and non-pregnant cows. Seven out of 8 non-pregnant cows showed significant CL c-s area regression between Days 14 and 20 (422 ± 112 v. 249 ± 63 mm2), whereas no regression was observed between Days 14 and 20 in pregnant cows (416 ± 65 v. 402 ± 78 mm2). The regression in the CL c-s area between pregnant and non-pregnant cows was significantly different during Day 18 (424 ± 65 v. 288 ± 88 mm2) to Day 23 (402 ± 71 v. 139 ± 64 mm2). P4 concentration was significantly low (less than 1 ng mL–1) at Day 20 in 3 out of 8 non-pregnant cows, whereas the pregnant cows showed significant increase of P4 between Days 14 and 20 (2.6 ± 0.2 v. 3.4 ± 0.5 ng mL–1). The pregnant cows showed significantly higher P4 concentration starting from Day 18 than non-pregnant cows. However, in non-pregnant cows, 4 cows returned to estrus on Day 20 or after, 3 cows showed no signs of estrus, and 1 cow came in estrus as early as Day 18 after AI. In conclusion, the results of the present study suggest that measuring the change in the CL c-s area at Days 14 and 20 makes it possible to detect the non-pregnant cows at Day 20 after AI. However, it was also indicated that measuring the change of P4 concentrations on the same days did not always successfully detect non-pregnant cows. The new protocol based on CL c-s area regression rate can detect almost certainly non-pregnant cows at Day 20 after AI. It is suggested that this method is advantageous in research and industrial breeding.

Use of milk progesterone enzyme immunoassay for early pregnancy diagnosis in cows

1982 ◽  
Vol 18 (3) ◽  
pp. 267-274 ◽  
Author(s):  
T. Nakao ◽  
A. Sugihashi ◽  
Y. Ishibashi ◽  
E. Tosa ◽  
Y. Nakagawa ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Early Pregnancy ◽  
Pregnancy Diagnosis

scholarly journals Early pregnancy diagnosis in pigs by enzyme immunoassay of serum progesterone.

1985 ◽  
Vol 31 (2) ◽  
pp. 68-73 ◽  
Author(s):  
Nobuhiko SAGA ◽  
Keiichiro KAWATA ◽  
Toshihiko NAKAO ◽  
Nobuo TSUNODA
Keyword(s):  
Enzyme Immunoassay ◽  
Early Pregnancy ◽  
Serum Progesterone ◽  
Pregnancy Diagnosis

scholarly journals Evaluation of synchronization protocols and methods of early pregnancy diagnosis in dairy cattle

2021 ◽  
Vol 50 (6) ◽  
Author(s):  
A.H. Shahzad ◽  
A. Sattar ◽  
N. Ahmad ◽  
I. Ahmad ◽  
M.S. Yousaf ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Early Pregnancy ◽  
Artificial Insemination ◽  
Pregnancy Loss ◽  
Holstein Cows ◽  
Plasma Progesterone ◽  
Pregnancy Diagnosis ◽  
Milk Samples ◽  
Timed Artificial Insemination ◽  
In Pregnancy

The studies aimed to evaluate the pregnancy rate (PR) for timed artificial insemination (TAI) after G7G-Ovsynch, modified G7G-Ovsynch (MG7G-Ovsynch) and Ovsynch protocols and to assess the accuracy of using pregnancy-associated glycoproteins (PAGs) and plasma progesterone (P4) in pregnancy diagnosis compared with ultrasonography (US). In study 1, Holstein cows (n = 37) were bred by TAI following the G7G-Ovsynch protocol (n = 19) or MG7G-Ovsynch (n = 18). Pregnancy was evaluated by US at days 31, 59, and 87 after breeding. The PR was not different for the G7G-Ovsynch and MG7G-Ovsynch. Blood and milk samples were collected on day 3 after insemination and then weekly through day 59 post TAI in cows diagnosed as not pregnant on day 31 and through day 87 in pregnant cows. PAGs were measured using ELISA and P4 by radioimmunoassay (RIA). In the second study, Holstein cows (n = 212) were bred by TAI following G7G-Ovsynch protocol (n = 110) or standard Ovsynch (n = 102). Cows were subjected to pregnancy diagnosis on days 30, 60, and 90. A subset (n = 15 in each group) was subjected to blood and milk samples on days 30, 45, 60, 75, and 90 to measure PAGs and P4. In study 2, PR was not significantly different between synchronization protocols on days 30, 60, and 90. Pregnancy loss averaged 15% between day 30 and day 90. The use of PAGs and P4 proved equally effective in diagnosis of pregnancy. Thus, G7G-Ovsynch was deemed the protocol of choice in postpartum cows, and PAGs assayed in milk or plasma could be used to diagnose pregnancy.

Early pregnancy diagnosis in buffaloes from plasma progesterone concentration

1980 ◽  
Vol 106 (5) ◽  
pp. 104-106 ◽  
Author(s):  
B. Perera ◽  
N. Pathiraja ◽  
S. Abeywardena ◽  
M. Motha ◽  
H. Abeygunawardena
Keyword(s):  
Early Pregnancy ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Pregnancy Diagnosis

Early Pregnancy Diagnosis Based upon Plasma Progesterone Levels in the Cow and Ewe

1973 ◽  
Vol 36 (4) ◽  
pp. 726-729 ◽  
Author(s):  
M. Skemesh ◽  
N. Ayalon ◽  
H. R. Lindner
Keyword(s):  
Early Pregnancy ◽  
Plasma Progesterone ◽  
Pregnancy Diagnosis

Increase in plasma progesterone caused by undernutrition during early pregnancy in the ewe

Reproduction ◽  
1971 ◽  
Vol 24 (1) ◽  
pp. 146-a-147 ◽  
Author(s):  
I. Cumming ◽  
B. Mole ◽  
J Obst ◽  
M. Blockey ◽  
C. Winfield ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Plasma Progesterone
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