Characteristics of Monoclonal Antibodies Against Human Chorionic Gonadotrophin

1982 ◽  
pp. 837-842 ◽  
Author(s):  
A.M.G. BOSCH ◽  
W. STEVENS ◽  
A. SCHUURS ◽  
O. SCHÖNHERR ◽  
H. ROELOFS
Keyword(s):  
Monoclonal Antibodies ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin

The production of monoclonal antibodies to human chorionic gonadotrophin and its subunits

1983 ◽  
Vol 98 (3) ◽  
pp. 323-330 ◽  
Author(s):  
M. C. Stuart ◽  
P. A. Underwood ◽  
D. F. Harman ◽  
K. L. Payne ◽  
D. A. Rathjen ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Free Form ◽  
Binding Affinities ◽  
Β Subunit ◽  
Α Subunit ◽  
Specific Antisera ◽  
Hybridoma Technology ◽  
Circulating Levels

The difficulties encountered in producing highly specific antisera to human chorionic gonadotrophin (hCG) were overcome by the use of hybridoma technology. A panel of monoclonal antibodies directed toward hCG and its subunits was produced. Of the four antibodies which were fully characterized, one recognized the intact hCG molecule only, a second recognized only the free β-subunit, a third recognized only the free α-subunit and the fourth bound to the β-subunit of hCG both when it was in the free form and when it was associated with the α-subunit forming the intact hCG molecule. There was no significant cross-reaction of any of these antibodies with the pituitary glycoprotein hormones. The four antibodies had high binding affinities which should permit their use in immunoassays for measurement of circulating levels of hCG and its subunits.

Monoclonal antibodies to the β-subunit of human chorionic gonadotrophin

1988 ◽  
Vol 106 (6) ◽  
pp. 1727-1729
Author(s):  
R. Fidler ◽  
A. V. Sokolov ◽  
M. Sh. Verbitskii ◽  
I. P. Papazov
Keyword(s):  
Monoclonal Antibodies ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Β Subunit

A homogeneous sol particle immunoassay for human chorionic gonadotrophin using monoclonal antibodies

1983 ◽  
Vol 60 (1-2) ◽  
pp. 9-23 ◽  
Author(s):  
Jan H.W. Leuvering ◽  
Bas C. Goverde ◽  
Piet J.H.M. Thal ◽  
Anton H.W.M. Schuurs
Keyword(s):  
Monoclonal Antibodies ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin

Monoclonal antibodies against the free subunits of human chorionic gonadotrophin

1990 ◽  
Vol 125 (2) ◽  
pp. 301-309 ◽  
Author(s):  
P. Berger ◽  
R. Klieber ◽  
W. Panmoung ◽  
S. Madersbacher ◽  
H. Wolf ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cross Reactivity ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Antigenic Determinants ◽  
Α Subunit ◽  
Α Chain ◽  
Fluid Levels ◽  
Α Subunits

ABSTRACT Discordant results on body fluid levels of human chorionic gonadotrophin (hCG) free α- and β-subunits under physiological and pathophysiological conditions, prompted us to raise a total of 260 monoclonal antibodies (MCA) against free hCG-α, free hCG-β, holo-hCG, human follicle-stimulating hormone and bovine luteinizing hormone; 153 MCA recognizing the human α-subunit and 28 reacting with hCG-β were extensively analysed for their intra- and interspecies cross-reactivity with homologous hormones, and for the compatibility of epitopes recognized by them. The immunological topography of free hCG-α and free hCG-β was resolved by these MCA, and epitope maps were designed. Six antigenic determinants on the free α-chain (α1–α6), clustered in three spatially distinct domains, and seven epitopes on the surface of free hCG-β (β1–β7), could be distinguished. Strikingly, three α-chain epitopes (α4, α5 and α6) were shared between various species, which is in contradiction to the concept of immunological species-specificity of α-subunits. Three determinants were found to be present only on the free subunits but not on holo-hCG (α6, β6 and β7), and only two determinants (β1 and β7) were hormone-specific for hCG. Based on this information, an immunoenzymometric assay for the free α-subunit of human glycoprotein hormones was established, with a sensitivity of 1·3 pg/well and a cross-reactivity with holo-hCG of less than 0·005% Thus this assay provides the basis for detecting free α-subunits in the presence of extremely high levels of holo-hormones, which may assist in elucidating the role of free α-subunits in man. Journal of Endocrinology (1990) 125, 301–309

Placental production of human chorionic gonadotrophin α and β subunits in early pregnancy as evidenced in fluid from the exocoelomic cavity

1994 ◽  
Vol 142 (3) ◽  
pp. 511-516 ◽  
Author(s):  
A-M Nagy ◽  
E Jauniaux ◽  
D Jurkovic ◽  
S Meuris
Keyword(s):  
Monoclonal Antibodies ◽  
Early Pregnancy ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Maternal Serum ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Molar Basis ◽  
Immunoreactive Band ◽  
Α And Β Subunits

Abstract Levels of human chorionic gonadotrophin (hCG) and of its free α and β subunits were measured using specific immunoradiometric assays in exocoelomic fluid (ECF) and maternal serum (MS) collected from five pregnant women at 6·6-8 weeks of gestation. Mean levels of hCG and its free subunits were significantly (P<0·001) higher in ECF than in MS: 3·5-fold for hCG, 600-fold for free αhCG and 38-fold for βhCG. There was no correlation between either hCG levels or levels of its free subunits in ECF and MS. On a molar basis, the quantity of free αhCG subunit expressed as a percentage of the total (free+combined) amount was 83% in ECF and 2·7% in MS (P<0·001). The amount of free βhCG subunit as a percentage of the total was 22% in ECF and 3·5% in MS (P<0·001). The ratio of the total amounts of α- and βhCG subunits amounted to 4·6 in ECF and 0·99 in MS (P<0·001). The heterogeneity of hCG was further investigated by polyacrylamide gel electrophoresis followed by immunoblotting. Several bands with molecular mass ranging from 42 to 57 kDa, corresponding to hCG dimers, were immunodetected in ECF and MS with anti-αhCG and anti-βhCG monoclonal antibodies. A free 35 kDa βhCG immunoreactive band was found in ECF and MS. A free αhCG immunoreactive band was observed at 23 kDa in ECF and at 21 kDa in MS. These findings suggest that the exocoelomic cavity is a reservoir where hCG and its subunits produced by trophoblast accumulate directly. The excess of a free heavy αhCG subunit in ECF supports the hypothesis that, during early pregnancy, there is an excess of αhCG over βhCG subunit secretion by the trophoblast. Journal of Endocrinology (1994) 142, 511–516

THE PRECISION OF THE OVARIAN ASCORBIC ACID DEPLETION TEST FOR LUTEINIZING HORMONE

1963 ◽  
Vol 43 (1) ◽  
pp. 155-160
Author(s):  
Jørgen Falck Larsen ◽  
Christian Hamburger
Keyword(s):  
Ascorbic Acid ◽  
Luteinizing Hormone ◽  
Clinical Analysis ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin

ABSTRACT Various modifications of the Parlow test for luteinizing hormone (ovarian ascorbic acid depletion in rats) were tried. Human chorionic gonadotrophin was used instead of hypophyseal luteinizing hormone. The precision of the method was found to be so low, however, that the test could not be used for routine clinical analysis. The low precision found in this and other laboratories is thought to be due to the strains of rats used.

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