Spread of recombinant Autographa californica nucleopolyhedrovirus in various tissues of silkworm Bombyx mori determined by real-time PCR

2008 ◽  
Vol 373 (1) ◽  
pp. 147-153 ◽  
Author(s):  
Yi Zhang ◽  
Baozhong Tian ◽  
Huanzhang Xia ◽  
Tingqing Guo ◽  
Jianyang Wang ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Real Time ◽  
Real Time Pcr ◽  
Autographa Californica ◽  
Autographa Californica Nucleopolyhedrovirus ◽  
Silkworm Bombyx Mori

Detection of silkworm (Bombyx mori) and Lepidoptera DNA in feeding stuff by real-time PCR

Food Control ◽  
2021 ◽  
Vol 126 ◽  
pp. 108059
Author(s):  
M. Zarske ◽  
J. Zagon ◽  
S. Schmolke ◽  
T. Seidler ◽  
A. Braeuning
Keyword(s):  
Bombyx Mori ◽  
Real Time ◽  
Real Time Pcr ◽  
Silkworm Bombyx Mori

Development of detection method for edible silkworm (Bombyx mori) using real-time PCR

Food Control ◽  
2018 ◽  
Vol 94 ◽  
pp. 295-299 ◽  
Author(s):  
Mi-Ju Kim ◽  
Seul-Ki Jung ◽  
Sung-Yeon Kim ◽  
Hae-Yeong Kim
Keyword(s):  
Bombyx Mori ◽  
Real Time ◽  
Real Time Pcr ◽  
Detection Method ◽  
Silkworm Bombyx Mori

scholarly journals Degradation of rRNA in BM-N cells from the silkworm Bombyx mori during abortive infection with heterologous nucleopolyhedroviruses

2013 ◽  
Vol 94 (9) ◽  
pp. 2102-2111 ◽  
Author(s):  
Rina Hamajima ◽  
Yuya Ito ◽  
Haruka Ichikawa ◽  
Hiroshi Mitsutake ◽  
Jun Kobayashi ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Spodoptera Exigua ◽  
Autographa Californica ◽  
Cosmid Library ◽  
S2 Cells ◽  
Abortive Infection ◽  
Hyphantria Cunea ◽  
Transient Expression Assay ◽  
Rrna Degradation ◽  
Silkworm Bombyx Mori

Cell lines derived from the silkworm, Bombyx mori, are only permissive for B. mori nucleopolyhedrovirus (NPV), with other NPVs generally resulting in abortive infection. Here, we demonstrate that rRNA of B. mori BM-N cells undergoes rapid degradation through site-specific cleavage upon infection with NPVs from Autographa californica (AcMNPV), Hyphantria cunea (HycuMNPV), Spodoptera exigua (SeMNPV) and Spodoptera litura (SpltMNPV). No significant decreases in cellular RNA were observed in Ld652Y, Se301, Sf9, SpIm and S2 cells infected with AcMNPV or HycuMNPV, indicating the response is unique to BM-N cells. A transient expression assay using a cosmid library of the HycuMNPV genome demonstrated that HycuMNPV P143 is responsible for rRNA degradation, which was also detected in BM-N cells transfected with plasmids expressing the P143 proteins from AcMNPV, SeMNPV and SpltMNPV. These results indicate that B. mori evolved to acquire a unique antiviral immune mechanism that is activated by P143 proteins from heterologous NPVs.

Selection and validation of reference genes for reverse transcription quantitative real-time PCR (RT-qPCR) in silkworm infected with Bombyx mori bidensovirus

Biologia ◽  
2018 ◽  
Vol 73 (9) ◽  
pp. 897-906 ◽  
Author(s):  
Zhaoyang Hu ◽  
Yanchun Deng ◽  
Xiaolong Zhang ◽  
Peipei Tang ◽  
Weijuan Sun ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr

Early and simultaneous detection ofNosema bombycis(Microsporidia: Nosematidae), nucleopolyhedrovirus (Baculoviridae), and densovirus (Parvoviridae) by multiplex real-time polymerase chain reaction inBombyx mori(Lepidoptera: Bombycidae)

2017 ◽  
Vol 149 (2) ◽  
pp. 265-275
Author(s):  
Shan Wu ◽  
Yong-Qiang He ◽  
Xing-Meng Lu ◽  
Xiao-Feng Zhang ◽  
Jiang-Bing Shuai ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Detection ◽  
Bombyx Mori ◽  
Real Time ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Post Inoculation ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Polymerase Chain

AbstractAn effective multiplex real-time polymerase chain reaction (PCR) assay for the simultaneous detection of three major pathogens,Nosema bombycisNägeli (Microsporidia: Nosematidae),Bombyx morinucleopolyhedrovirus (Baculoviridae: genusAlphabaculovirus) (NPV), andBombyx moridensovirus (Parvoviridae: genusIteravirus) (DNV), in silkworms (Bombyx mori(Linnaeus); Lepidoptera: Bombycidae) was developed in this study. Polymerase chain reaction and real-time PCR tests and basic local alignment search tool searches revealed that the primers and probes used in this study had high specificities for their target species. The ability of each primer/probe set to detect pure pathogen DNA was determined using a plasmid dilution panel, in which under optimal conditions the multiplex real-time PCR assay showed high efficiency in the detection of three mixed target plasmids with a detection limit of 8.5×103copies forN. bombycisandBombyx moriNPV (BmNPV) and 8.5×104copies forBombyx moriDNV (BmDNV). When the ability to detect these three pathogens was examined in artificially inoculated silkworms, our method presented a number of advantages over traditional microscopy, including specificity, sensitivity, and high-throughput capabilities. Under the optimal volume ratio for the three primer/probe sets (3:2:2=N. bombycis:BmNPV:BmDNV), the multiplex real-time PCR assay showed early detection of BmNPV and BmDNV by day 1 post inoculation using DNA templates of the three pathogens in various combinations from individually infected silkworms; the early detection ofN. bombyciswas possible by day 3 post inoculation using the DNA isolated from the midgut ofN. bombycis-infected silkworms.

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