Deciphering the structure of isomeric oligosaccharides in a complex mixture by tandem mass spectrometry: Photon activation with vacuum ultra-violet brings unique information and enables definitive structure assignment

2014 ◽  
Vol 807 ◽  
pp. 84-95 ◽  
Author(s):  
David Ropartz ◽  
Jérôme Lemoine ◽  
Alexandre Giuliani ◽  
Yann Bittebière ◽  
Quentin Enjalbert ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Complex Mixture ◽  
Ultra Violet ◽  
Tandem Mass ◽  
Photon Activation ◽  
Unique Information ◽  
Structure Assignment ◽  
Vacuum Ultra Violet

Sensitive, specific quantitative analysis of tacrolimus (FK506) in blood by liquid chromatography-electrospray tandem mass spectrometry

1996 ◽  
Vol 42 (2) ◽  
pp. 279-285 ◽  
Author(s):  
P J Taylor ◽  
A Jones ◽  
G A Balderson ◽  
S V Lynch ◽  
R L Norris ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Complex Mixture ◽  
Turnaround Time ◽  
Pharmacokinetic Studies ◽  
Tandem Mass ◽  
Blood Concentrations ◽  
Liquid Chromatography Tandem Mass ◽  
The Difference

Abstract The capacity of liquid chromatography-tandem mass spectrometry (LC-MS2) to detect and define individual components in a complex mixture has been utilized to develop a quantitative assay of the potent immunosuppressant drug, tacrolimus. Trough blood concentrations were measured in 175 samples obtained over several weeks after liver transplantation from seven patients. The assay was linear over the range of 0.2 to 100 micrograms/L. Imprecision was <8%, and accuracy was 99-101%. The turnaround time for a batch of 20 samples was 2.5 h. No interference from any of the other drugs being administered to the patients was evident. An ELISA also performed on the same samples overestimated the concentrations substantially, as indicated by a plot of the difference between the results for the two methods vs their mean. The favorable characteristics of the LC-MS2 assay, especially its sensitivity and specificity, will facilitate detailed pharmacokinetic studies of tacrolimus, particularly under circumstances in which metabolism is perturbed by either hepatic dysfunction or drug interactions.

scholarly journals Automated Lipid A Structure Assignment from Hierarchical Tandem Mass Spectrometry Data

2011 ◽  
Vol 22 (5) ◽  
pp. 856-866 ◽  
Author(s):  
Ying S. Ting ◽  
Scott A. Shaffer ◽  
Jace W. Jones ◽  
Wailap V. Ng ◽  
Robert K. Ernst ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Lipid A ◽  
Mass Spectrometry Data ◽  
Tandem Mass ◽  
Lipid A Structure ◽  
Structure Assignment ◽  
Tandem Mass Spectrometry Data

Determination of toxic cyclic heptapeptides by liquid chromatography with detection using ultra-violet, protein phosphatase assay and tandem mass spectrometry

Chemosphere ◽  
2004 ◽  
Vol 55 (10) ◽  
pp. 1395-1402 ◽  
Author(s):  
Paloma Muñiz Ortea ◽  
Orla Allis ◽  
Brendan M Healy ◽  
Mary Lehane ◽  
Aine Nı́ Shuilleabháin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Protein Phosphatase ◽  
Ultra Violet ◽  
Tandem Mass ◽  
Phosphatase Assay
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