Effect of nutritional state on semen characteristics, testicular size and serum testosterone concentration in Sanjabi ram lambs during the natural breeding season

2015 ◽  
Vol 153 ◽  
pp. 22-28 ◽  
Author(s):  
F. Ghorbankhani ◽  
M. Souri ◽  
M.M. Moeini ◽  
R. Mirmahmoudi
Author(s):  
Yamina Belkhiri ◽  
Farida Bouzebda-Afri ◽  
Zoubir Bouzebda ◽  
Charef Eddine Mouffok ◽  
Amal Djaout

This experiment was carried out on Ouled Djellal rams in order to study the effect of season on testicular size, serum testosterone level and semen characteristics for a period of one year. Blood samples were obtained via jugular vein, and semen was collected by using an electro-ejaculator from six fertile male rams (2-4 years of age) on a monthly basis. In this breed, testis parameters varied seasonally showing the highest average values during autumn. However, the variation of body weight, GSI, serum testosterone levels, mass motility, individual motility and sperm concentration were not significant during the sampling period. The results reported show that the lowest and highest levels of semen volume were recorded in winter (0.62±0.11 ml) and in autumn (1.23±0.09 ml) respectively. The pH value and total number in sperm showed a uniform pattern with low scores during breeding season and high scores in non-breeding season. High percentage of live sperm was recorded during the summer, and high percentage of abnormal sperm was recorded during winter.


Author(s):  
R. Selvakkumar ◽  
T. Sivakumar ◽  
S. Ganesh Kumar ◽  
P. Anuradha

The present investigation was carried out to study the prepubertal testosterone pattern and age at puberty in Vembur rams reared under farm and farmers field condition. A total number of 8 Vembur ram lambs each from farm and farmers field were selected. The measurement of testosterone was performed by Radioimmunoassay (RIA). The serum testosterone level from 6th to 20th month of age ranged from 0.132 ± 0.04 to 3.823 ng/ml in semi intensively maintained farm males and 0.138±0.04 to 3.456±0.17 ng/ml in animals maintained at farmer’s holdings. The serum testosterone levels were crossing the concentration of 2.5 ng/ml at the age of 17 to 18th weeks and successful mounting, intromission and ejaculation were noticed after this age.


Author(s):  
Ahmed A. Al-kawmani ◽  
Mansour M. Alfuraiji ◽  
Saleh A. Kandeal ◽  
Mohammad Abul Farah ◽  
Khalid Mashai Alanazi

The present work was conducted to elucidate the relationships between chronological age, testosterone concentration and testis size of Najdi and Naemi ram lambs under desert conditions to determine the reproductive capacity of the above two breeds. The postnatal development of the seminiferous epithelium of the testes was also monitored. A total of 90 (45 Najdi and 45 Naemi lambs) aged between one and nine months, with five lambs at each month of age for each breed were used. The results showed that the testosterone concentration increased dramatically and rapidly between five and six months, followed by a period of gradual growth where testicular size was increased from 3 to 6 months of age. There was a significant (P > 0.05) difference in testosterone concentration (TC) and testis size (TS) in ram lambs between different age groups, similarly there were significant differences between body weights and lamb age between one and nine months. A positive correlation between the increasing body weight and the size of the testicle was observed. The testicles of lambs at each age-group had a different histological appearance except those of Najdi lambs at 2, 3 and 4 months and Naemi lambs at 2, 3, 4 and 5 months, where few differences were apparent. Small quantities were of spermatozoa appeared at the eighth month of age and increased in the ninth month. In conclusion, the current findings indicate that puberty in Najdi and Naemi rams occurs at the 8th and 9th month of age, respectively.


2019 ◽  
Vol 12 (1) ◽  
pp. 74-88
Author(s):  
Maad AL-Ameri ◽  
Talal Abdulkareem ◽  
Ahmed Taha

The current study aimed to determine the effect of hormonal treatment with kisspeptin, GnRH and hCG on plasma testosterone concentration and semen characteristics in buck Cyprus goats during non-breeding season as compared with breeding season. This study was executed at the Ruminant Researches Station pertaining to the Directorate of Agricultural Researches, Ministry of Agriculture, Abu-Ghraib, Baghdad (latitude 33˚20' N) for the period from November 15th, 2012 to December 〖31〗^st 2013. A total of 20 buck Cyprus goats 2 years old and averages 55 kg body weight. During non-breeding season bucks were randomly divided into five equal groups (4 bucks / group). The first group (A1) was regarded as a control group, i.m injected with normal saline, whereas, the second (A2) and third (A3) groups were i.v injected with 4 and 8 µg / kg body weight of Kisspeptin-10 respectively. The fourth (A4) and fifth (A5) groups were i.m injected with hCG (250 IU / buck) and GnRH (20 µg / buck) respectively. Plasma testosterone concentrations were significantly (P≤ 0.01 and P≤ 0.05) increased at 20, 30, 40 and 50 min post-treatment in A5, A2 and A3 groups as compared with control A1. Mass motility and individual motility were significantly (P≤ 0.01) in A5, A4, A2 and A3 as compared with control during non-breeding season. In conclusion, our results show that Kisspeptin-10, GnRH and hCG can stimulate the quiescent hypothalamic–pituitary–gonadal axis of bucks during non-breeding season by increasing plasma testosterone concentration post treatment that leads improving some semen characteristics during non-breeding season.


2020 ◽  
Vol 32 (2) ◽  
pp. 198
Author(s):  
A. K. Chaudhary ◽  
G. N. Purohit ◽  
J. S. Mehta ◽  
S. K. Ravi ◽  
T. R. Talluri

The present study investigated the serum testosterone profile of Marwari stallions before and after exposure to a mare and the relationship of serum testosterone profile with scrotal circumference, semen characteristics, reaction time, stallion age, bodyweight, and height. Marwari stallions (n=9) of three age groups (2-4 years, n=3; 4-6 years, n=3; and 6+ years, n=3) were used in the study. Scrotal circumference, height, and bodyweight of each of the stallions were measured. Semen was collected from each stallion twice per week in the early-morning hours using an artificial vagina and a mare in oestrus. Six ejaculates were collected from each stallion for evaluation of various seminal parameters (semen volume, sperm concentration, and progressive sperm motility). Reaction time for each stallion was also recorded. At every alternate semen collection (first, third, and fifth collections), blood samples were taken 15min before exposure to the mare and just before the semen collection for serum testosterone hormone assay using a horse testosterone enzyme-linked immunosorbent assay kit. The data obtained were analysed statistically using SPSS ver. 20.0 (IBM Corp.). The results showed that mean testosterone concentration was significantly different (P ≤ 0.01) among the stallions and was significantly lower (P ≤ 0.01) in the stallions below 4 years of age. No significant difference in testosterone level was observed before and after exposure to a mare. A positive correlation was detected between testosterone and both scrotal circumference (P ≤ 0.05) and sperm concentration (P ≤ 0.05), whereas a negative correlation was observed with reaction time (P ≤ 0.01). It was concluded that exposure to a mare does not change the testosterone level in stallion blood and that there is a relationship of serum testosterone concentration with scrotal circumference, sperm concentration, reaction time, and age but not with height and bodyweight.


1986 ◽  
Vol 15 (6) ◽  
pp. 419-432 ◽  
Author(s):  
Margaret R. Clarke ◽  
Jay R. Kaplan ◽  
Patricia T. Bumsted ◽  
Donald R. Koritnik

1993 ◽  
Vol 137 (2) ◽  
pp. 247-NP ◽  
Author(s):  
H. F. Urbanski ◽  
M. M. Fahy ◽  
P. M. Collins

ABSTRACT The influence of excitatory amino acids (EAAs) on reproductive neuroendocrine function was investigated in adult male Syrian hamsters of the LSH/Ss Lak strain. Before the study, the animals were maintained in a sexually regressed condition, under short days (SD) and subsequently were either transferred to long days (LD) or kept under SD, for a further 4 weeks. In the former group, photostimulation produced a predictable elevation in the hypophysial contents and serum concentrations of FSH and LH. This was accompanied by an increase in testicular size, an elevation in serum testosterone levels and an increase in spermatogenic activity; the SD hamsters remained sexually quiescent throughout the study. In contrast, SD hamsters that were given daily injections of the EAA agonist, N-methyl-d,l-aspartate (NMA: 50 mg/kg body weight, s.c.), showed stimulatory responses that were generally even more pronounced than those shown by the LD group. Surprisingly, an identical NMA treatment paradigm failed to cause a similar activation of the reproductive axis in LD hamsters that were given daily afternoon injections of melatonin (25 μg, s.c), even though the inhibitory effect of this melatonin treatment is generally regarded as being comparable with that produced by exposure to SD. Although EAAs can acutely stimulate the neurocircuitry that controls LH-releasing hormone secretion, the present findings suggest that EAAs might also exert a long-term stimulatory action by acting further upstream in the photoneuroendocrine pathway. Journal of Endocrinology (1993) 137, 247–252


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