scholarly journals Chemical constituents and nitric oxide inhibitory activity of supercritical carbon dioxide extracts from Mitragyna speciosa leaves

2019 ◽  
Vol 12 (3) ◽  
pp. 350-359 ◽  
Author(s):  
Norsita Tohar ◽  
Jamil A. Shilpi ◽  
Yasodha Sivasothy ◽  
Syahida Ahmad ◽  
Khalijah Awang
Keyword(s):  
Nitric Oxide ◽  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Inhibitory Activity ◽  
Chemical Constituents ◽  
Mitragyna Speciosa ◽  
Supercritical Carbon

scholarly journals Compound Identification and In Vitro Cytotoxicity of the Supercritical Carbon Dioxide Extract of Papaya Freeze-Dried Leaf Juice

Processes ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 610
Author(s):  
Kooi-Yeong Khaw ◽  
Paul Nicholas Shaw ◽  
Marie-Odile Parat ◽  
Saurabh Pandey ◽  
James Robert Falconer
Keyword(s):  
Mass Spectrometry ◽  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Chemical Constituents ◽  
Gas Chromatography Mass Spectrometry ◽  
Cytotoxic Activities ◽  
Compound Identification ◽  
Freeze Dried ◽  
Leaf Juice ◽  
Supercritical Carbon

Carica papaya leaves are used as a remedy for the management of cancer. Freeze-dried C. papaya leaf juice was extracted using a supercritical fluid extraction system. Compound identification was carried out using analytical techniques including liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry (LC–QToF-MS) and gas chromatography–mass spectrometry (GC–MS). The cytotoxic activities of the scCO2 extract and its chemical constituents were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on squamous cell carcinoma (SCC25) and human keratinocyte (HaCaT) cell lines. The chemical constituents were quantified by QToF-MS. The supercritical carbon dioxide (scCO2) extract of papaya freeze-dried leaf juice showed cytotoxic activity against SCC25. Three phytosterols, namely, β-sitosterol, campesterol, and stigmasterol, together with α-tocopherol, were confirmed to be present in the scCO2 extract. Quantitative analysis showed that β-sitosterol was the major phytosterol present followed by α-tocopherol, campesterol, and stigmasterol. β-Sitosterol and campesterol were active against SCC25 (half maximal inhibitory concentration (IC50) ≈ 1 µM), while stigmasterol was less active (~33 µM) but was biologically more selective against SCC25. Interestingly, an equimolar mixture of phytosterols was not more effective (no synergistic effect was observed) but was more selective than the individual compounds. The compounds identified are likely accountable for at least part of the cytotoxicity and selectivity effects of C. papaya.

Cytotoxic, antimicrobial and nitric oxide inhibitory activities of supercritical carbon dioxide extracted Prunus persica leaves

2019 ◽  
Vol 47 (1) ◽  
pp. 569-581 ◽  
Author(s):  
Halil Koyu ◽  
Aslihan Kazan ◽  
Ayse Nalbantsoy ◽  
Husniye Tansel Yalcin ◽  
Ozlem Yesil-Celiktas
Keyword(s):  
Nitric Oxide ◽  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Prunus Persica ◽  
Inhibitory Activities ◽  
Supercritical Carbon
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