Cancer prevention with semi-allogeneic ES cell-derived dendritic cells

2005 ◽  
Vol 335 (1) ◽  
pp. 5-13 ◽  
Author(s):  
Daiki Fukuma ◽  
Hidetake Matsuyoshi ◽  
Shinya Hirata ◽  
Akari Kurisaki ◽  
Yutaka Motomura ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cancer Prevention ◽  
Es Cell

Generation and genetic modification of dendritic cells derived from mouse embryonic stem cells

Blood ◽  
2003 ◽  
Vol 101 (9) ◽  
pp. 3501-3508 ◽  
Author(s):  
Satoru Senju ◽  
Shinya Hirata ◽  
Hidetake Matsuyoshi ◽  
Masako Masuda ◽  
Yasushi Uemura ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Bone Marrow Cells ◽  
Es Cells ◽  
Embryonic Stem ◽  
Gene Trap ◽  
Genetically Engineered ◽  
Interleukin 4 ◽  
Es Cell ◽  
Entry Site

We developed a method to generate dendritic cells (DCs) from mouse embryonic stem (ES) cells. We cultured ES cells for 10 days on feeder cell layers of OP9, in the presence of granulocyte-macrophage colony-stimulating factor in the latter 5 days. The resultant ES cell–derived cells were transferred to bacteriologic Petri dishes without feeder cells and further cultured. In about 7 days, irregularly shaped floating cells with protrusions appeared and these expressed major histocompatibility complex class II, CD11c, CD80, and CD86, with the capacity to stimulate primary mixed lymphocyte reaction (MLR) and to process and present protein antigen to T cells. We designated them ES-DCs (ES cell–derived dendritic cells), and the functions of ES-DCs were comparable with those of DCs generated from bone marrow cells. Upon transfer to new dishes and stimulation with interleukin-4 plus tumor necrosis factor α, combined with anti-CD40 monoclonal antibody or lipopolysaccharide, ES-DCs completely became mature DCs, characterized by a typical morphology and higher capacity to stimulate MLR. Using an expression vector containing the internal ribosomal entry site–puromycinN-acetyltransferase gene or a Cre-lox–mediated exchangeable gene-trap system, we could efficiently generate ES cell transfectants expressing the products of introduced genes after their differentiation to DCs. ES-DCs expressing invariant chain fused to a pigeon cytochrome C epitope presented the epitope efficiently in the context of Ek. We primed ovalbumin (OVA)–specific cytotoxic T lymphocytes in vivo by injecting mice with ES-DCs expressing OVA, thus demonstrating immunization with ES-DCs genetically engineered to express antigenic protein. The methods may be applicable to immunomodulation therapy and gene-trap investigations of DCs.

Dendritic cells of the human epidermis: immuno-electron microscopic studies of la antigen reactivity

1978 ◽  
Vol 36 (2) ◽  
pp. 644-645
Author(s):  
G. Rowden ◽  
M. G. Lewis ◽  
T. M. Phillips
Keyword(s):  
Dendritic Cells ◽  
Langerhans Cells ◽  
Cell Types ◽  
Cell Type ◽  
Electron Microscopic ◽  
La Antigen ◽  
Microscopic Studies ◽  
A Cell ◽  
C3 Receptors ◽  
Antigen Reactivity

Langerhans cells of mammalian stratified squamous epithelial have proven to be an enigma since their discovery in 1868. These dendritic suprabasal cells have been considered as related to melanocytes either as effete cells, or as post divisional products. Although grafting experiments seemed to demonstrate the independence of the cell types, much confusion still exists. The presence in the epidermis of a cell type with morphological features seemingly shared by melanocytes and Langerhans cells has been especially troublesome. This so called "indeterminate", or " -dendritic cell" lacks both Langerhans cells granules and melanosomes, yet it is clearly not a keratinocyte. Suggestions have been made that it is related to either Langerhans cells or melanocyte. Recent studies have unequivocally demonstrated that Langerhans cells are independent cells with immune function. They display Fc and C3 receptors on their surface as well as la (immune region associated) antigens.

Human PMNs are phagocytosed by dendritic cells and interfere with DCs maturation: a new link between innate and acquired immunity

2010 ◽  
Vol 34 (8) ◽  
pp. S72-S72
Author(s):  
Qi Xu ◽  
Xiumin Ma ◽  
Yuejie Zhu ◽  
Fengsen Li ◽  
Jianbing Ding
Keyword(s):  
Dendritic Cells ◽  
Acquired Immunity ◽  
Human Pmns

Spotlight Cancer Prevention in April

ASHA Leader ◽  
2010 ◽  
Vol 15 (3) ◽  
pp. 2-2 ◽  
Author(s):  
Terry Day
Keyword(s):  
Cancer Prevention

Generation of functionally mature dendritic cells from the multipotential stem cell line FDCP-mix

2000 ◽  
Vol 111 (3) ◽  
pp. 890-897 ◽  
Author(s):  
Timm Schroeder ◽  
Claudia Lange ◽  
John Strehl ◽  
Ursula Just
Keyword(s):  
Dendritic Cells ◽  
Stem Cell ◽  
Cell Line ◽  
Stem Cell Line
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