Inhibition of the high affinity choline transporter enhances hyperalgesia in a rat model of chronic pancreatitis

2017 ◽  
Vol 488 (1) ◽  
pp. 204-210 ◽  
Author(s):  
Dan Luo ◽  
Lei Chen ◽  
Baoping Yu
Keyword(s):  
Chronic Pancreatitis ◽  
Rat Model ◽  
Choline Transporter ◽  
High Affinity ◽  
High Affinity Choline Transporter

Localisation of the high-affinity choline transporter-1 in the rat skeletal motor unit

2002 ◽  
Vol 307 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Katrin Lips ◽  
Uwe Pfeil ◽  
Rainer Haberberger ◽  
Wolfgang Kummer
Keyword(s):  
Motor Unit ◽  
Choline Transporter ◽  
High Affinity ◽  
High Affinity Choline Transporter

scholarly journals Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Chen Zhao ◽  
Mengjuan Lin ◽  
Yasi Pan ◽  
Baoping Yu
Keyword(s):  
Normal Saline ◽  
Specific Inhibitor ◽  
Visceral Hypersensitivity ◽  
Choline Uptake ◽  
Uptake System ◽  
Threshold Intensity ◽  
Choline Transporter ◽  
High Affinity ◽  
Acetylcholine Concentration ◽  
High Affinity Choline Transporter

Background. Visceral hypersensitivity is a common feature of irritable bowel syndrome. Cholinergic system involves in the development of visceral hypersensitivity, and high-affinity choline transporter (CHT1) is of crucial importance in choline uptake system. However, involvement of CHT1 in visceral hypersensitivity remains unknown. The research aimed to study the CHT1 expression in dorsal root ganglions (DRGs) and the role of CHT1 in visceral hypersensitivity. Methods. Repetitive water avoidance stress (WAS) was used to induce visceral hypersensitivity in rats. Colorectal distension (CRD) was determined, and the abdominal withdrawal reflex (AWR) and threshold intensity data were recorded to measure the visceral sensitivity. After intraperitoneal injection of hemicholinium-3 (HC-3), the specific inhibitor of CHT1, CRD data were also recorded. The CHT1 expression of DRGs was investigated by Western blotting, immunohistochemistry, and quantitative RT-PCR. Acetylcholine levels in the DRGs were detected by the assay kit. Results. Repetitive WAS increased the AWR score of CRD at high distension pressure and decreased the mean threshold of rats. The CHT1 expression and acetylcholine concentration of DRG were significantly increased in WAS rats. After the administration of HC-3, the AWR score in WAS group was significantly increased at higher distension pressure while the threshold intensity was significantly reduced compared to the normal saline group. Acetylcholine concentration was significantly lower than the normal saline rats. Conclusion. Our research firstly reports that CHT1 is overexpressed in noninflammatory visceral hypersensitivity, and blockage of CHT1 can enhance the visceral hypersensitivity. CHT1 may play an inhibitory role in visceral hypersensitivity.

scholarly journals Constitutive high-affinity choline transporter endocytosis is determined by a carboxyl-terminal tail dileucine motif

2005 ◽  
Vol 94 (1) ◽  
pp. 86-96 ◽  
Author(s):  
Fabiola M. Ribeiro ◽  
Stefanie A. G. Black ◽  
Sean P. Cregan ◽  
Vania F. Prado ◽  
Marco A. M. Prado ◽  
...  
Keyword(s):  
Choline Transporter ◽  
High Affinity ◽  
Dileucine Motif ◽  
Carboxyl Terminal ◽  
High Affinity Choline Transporter

Expression of the high-affinity choline transporter CHT1 in epithelia

Life Sciences ◽  
2003 ◽  
Vol 72 (18-19) ◽  
pp. 2087-2090 ◽  
Author(s):  
Uwe Pfeil ◽  
Rainer V Haberberger ◽  
Katrin S Lips ◽  
Lars Eberling ◽  
Veronika Grau ◽  
...  
Keyword(s):  
Choline Transporter ◽  
High Affinity ◽  
High Affinity Choline Transporter
Sign in / Sign up

Export Citation Format

Share Document