The effect of a laminin-5-derived peptide coated onto chitin microfibers on re-epithelialization in early-stage wound healing

Biomaterials ◽  
2010 ◽  
Vol 31 (17) ◽  
pp. 4725-4730 ◽  
Author(s):  
Seung-Ki Min ◽  
Sang-Chul Lee ◽  
Seong-Doo Hong ◽  
Chong-Pyoung Chung ◽  
Won Ho Park ◽  
...  
Keyword(s):  
Wound Healing ◽  
Early Stage ◽  
Laminin 5

scholarly journals N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study

2021 ◽  
Vol 22 (14) ◽  
pp. 7549
Author(s):  
Wiktor Paskal ◽  
Adriana M. Paskal ◽  
Piotr Pietruski ◽  
Albert Stachura ◽  
Kacper Pełka ◽  
...  
Keyword(s):  
Wound Healing ◽  
Early Stage ◽  
Healing Process ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Time Points ◽  
Model Study ◽  
Sprague Dawley Rats ◽  
Animal Model Study ◽  
Anesthetic Solution

The aim of the study was to evaluate if a pre-incisional N-acetylcysteine (NAC) treatment altered the process of wound healing in a rat model. The dorsal skin of 24 Sprague-Dawley rats was incised in six locations. Before the incisions were made, skin was injected either with lidocaine and epinephrine (one side) or with these agents supplemented with 0.015%, 0.03%, or 0.045% NAC (contralaterally). Photographic documentation of the wound healing process was made at 11 time points. Rats were sacrificed 3, 7, 14, or 60 days after incision to excise scars for histological analysis. They included: Abramov scale scoring, histomorphometry analysis, and collagen fiber arrangement assessment. Skin pretreated with 0.03% NAC produced the shortest scars at all analyzed time points, though this result was statistically insignificant. At this NAC concentration the scars had smaller areas on the third day and were narrower on the day 4 compared with all the other groups (p < 0.05). On day 7, at the same concentration of NAC, the scars had a higher superficial concentration index (p = 0.03) and larger dermal proliferation area (p = 0.04). NAC addition to pre-incisional anesthetic solution decreased wound size and width at an early stage of scar formation at all concentrations; however, with optimal results at 0.03% concentration.

scholarly journals Topical Application of Ozonated Oils for the Treatment of MRSA Skin Infection in an Animal Model of Infected Ulcer

Biology ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 372
Author(s):  
Vanessa Silva ◽  
Cecília Peirone ◽  
Rosa Capita ◽  
Carlos Alonso-Calleja ◽  
José A Marques-Magallanes ◽  
...  
Keyword(s):  
Wound Healing ◽  
Animal Model ◽  
Topical Application ◽  
Early Stage ◽  
Bacterial Load ◽  
Ozone Treatment ◽  
Diabetic Patients ◽  
Colony Forming Units ◽  
Group 2 ◽  
Epidermal Regeneration

Diabetic foot ulcers are a common cause of morbidity in diabetic patients. One of the main pathogens found in these ulcers is methicillin-resistant Staphylococcus aureus (MRSA). MRSA often carries resistance to several classes of antibiotics and their infections are becoming harder to treat. Therefore, new alternatives are urgently needed. Thus, this study aimed to investigate the capacity of topical ozonated oil application on the treatment of early-stage skin infected with MRSA in an animal model. Ozonated oil was prepared from a mixture of oils subjected to a gas stream of O2/O3 mixture. Sixteen Wistar rats were inoculated by an intradermic injection of MRSA suspension, producing an abscess lesion. After 3 days, the skin epidermis was removed to open the wound. Group 1 received an application of oil mixture without ozone treatment and Group 2 received an application of ozonated oil. After the treatment period, skin was collected, colony-forming units (CFU) of bacteria were quantified and the histological analysis of the skin was carried out. Skin samples from the control 1 and 2 had a bacterial load was of 1.1 × 105 and 5.7 × 103 CFU/mL, respectively. Group 2 showed better wound healing from mild to moderate epidermal regeneration. Topical application of ozonated vegetable oil in MRSA-infected skin in rats showed a small reduction of the bacterial load and better wound healing.

scholarly journals Effect of Dietary Conjugated Linoleic Acid Supplementation on Early Inflammatory Responses during Cutaneous Wound Healing

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Na-Young Park ◽  
Giuseppe Valacchi ◽  
Yunsook Lim
Keyword(s):  
Oxidative Stress ◽  
Wound Healing ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Wound Closure ◽  
Inflammatory Responses ◽  
Early Stage ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Anti Inflammatory

Inflammatory response is considered the most important period that regulates the entire healing process. Conjugated linoleic acid (CLA), a class of linoleic acid positional and geometric isomers, is well known for its antioxidant and anti-inflammatory properties. We hypothesized that dietary CLA supplementation accelerates cutaneous wound healing by regulating antioxidant and anti-inflammatory functions. To investigate wound closure rates and inflammatory responses, we used a full-thickness excisional wound model after 2-week treatments with control, 0.5%, or 1% CLA-supplemented diet. Mice fed dietary CLA supplementation had reduced levels of oxidative stress and inflammatory markers. Moreover, the wound closure rate was improved significantly in mice fed a 1% CLA-supplemented diet during early stage of wound healing (inflammatory stage). We conclude that dietary CLA supplementation enhances the early stage of cutaneous wound healing as a result of modulating oxidative stress and inflammatory responses.

High‐dose folic acid and its effect on early stage diabetic foot ulcer wound healing

2020 ◽  
Vol 28 (4) ◽  
pp. 517-525
Author(s):  
Joseph V. Boykin ◽  
Glenn D. Hoke ◽  
Cassandra R. Driscoll ◽  
Benita S. Dharmaraj
Keyword(s):  
Wound Healing ◽  
Folic Acid ◽  
Diabetic Foot ◽  
Early Stage ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
High Dose

Electrospinning of collagen nanofibers: Effects on the behavior of normal human keratinocytes and early-stage wound healing

Biomaterials ◽  
2006 ◽  
Vol 27 (8) ◽  
pp. 1452-1461 ◽  
Author(s):  
Kyong Su Rho ◽  
Lim Jeong ◽  
Gene Lee ◽  
Byoung-Moo Seo ◽  
Yoon Jeong Park ◽  
...  
Keyword(s):  
Wound Healing ◽  
Early Stage ◽  
Human Keratinocytes ◽  
Normal Human Keratinocytes ◽  
Normal Human

scholarly journals Emerging Technologies in Scar Management: Remodeling of Post-surgical Linear Scar Using Microplasma Radiofrequency

2020 ◽  
pp. 465-473
Author(s):  
Wei Liu
Keyword(s):  
Wound Healing ◽  
Early Stage ◽  
Scar Tissue ◽  
Natural Process ◽  
Background Information ◽  
Psychological Disturbance ◽  
Treatment And Prevention ◽  
Scar Management ◽  
Histological Architecture ◽  
Linear Scar

AbstractLinear scar is a common problem in all fields of surgery as it leads to unpleasant appearance that is not socially acceptable. In particular, linear scars in the exposed areas such as face, neck, and upper extremities cause significant psychological disturbance to patients who need proper medical assistance to release their suffering. The author proposed tissue remodeling strategy on an existed linear scar using FMRT (fractional microplasma radiofrequency technology) to render gross view of a linear scar less visible or non-visible by changing the scar tissue architecture. Similar concept is also applied to the intervention of an early-stage wound to significantly alter the natural process of wound healing via FMRT-mediated change of the histological architecture of a wound, and thus to prevent the formation of a grossly visible linear scar. This chapter introduces the general background information, FMRT concept, and its application on linear scar treatment and prevention with the presentation of several typical cases.

scholarly journals Effect of Allogeneic Oral Mucosa Mesenchymal Stromal Cells on Equine Wound Repair

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Paola Di Francesco ◽  
Pauline Cajon ◽  
Christophe Desterke ◽  
Marie-France Perron Lepage ◽  
Jean-Jacques Lataillade ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wound Repair ◽  
Positive Impact ◽  
Early Stage ◽  
Full Thickness ◽  
Skin Punch Biopsy ◽  
Significant Difference

Objective. To assess the clinical value and safety of the application of allogeneic equine oral mucosa mesenchymal stromal cells (OM-MSCs) to wounds. Animals. 8 healthy adult horses without front limb skin lesions or musculoskeletal disease. Procedures. Stem cells were isolated from the oral mucosa of a donor horse. Horses were subjected to the creation of eight full-thickness cutaneous wounds, two on each distal forelimb (FL) and two on both sides of the thorax (TH). Each wound was subjected to one out of four treatments: no medication (T1), hyaluronic acid- (HA-) gel containing OM-MSC (T2), HA-gel containing OM-MSC secretome (T3), and HA-gel alone (T4). Gross macroscopic evaluation and laser digital photographic documentation were regularly performed to allow wound assessment including wound surface area. Full-thickness skin punch biopsy was performed at each site before wound induction (D0, normal skin) and after complete wound healing (D62, repaired skin). Results. All wounds healed without adverse effect at D62. Distal limb wounds are slower to heal than body wounds. OM-MSC and its secretome have a positive impact on TH wound contraction. OM-MSC has a positive impact on the contraction and epithelialization of FL wounds. No significant difference between wound sites before and after treatment was noted at histological examination. Conclusion and Clinical Relevance. Using horse cells harvested from oral mucosa is a feasible technique to produce OM-MSC or its secretome. The gel produced by the combination of these biologic components with HA shows a positive impact when applied during the early stage of wound healing.

The Role of Leukocyte-Platelet-Rich Fibrin in Promoting Wound Healing in Diabetic Foot Ulcers

2021 ◽  
pp. 153473462110528
Author(s):  
Yuqi Wang ◽  
Xiaotao Wang ◽  
Rong Chen ◽  
Liuwei Gu ◽  
Desen Liu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Diabetic Foot ◽  
Early Stage ◽  
Treatment Method ◽  
Foot Ulcers ◽  
Control Group ◽  
Diabetic Foot Ulcers ◽  
External Application ◽  
Traditional Treatment ◽  
Platelet Rich Fibrin

To explore the effect of leukocyte-platelet-rich fibrin (L-PRF) on promoting wound healing in diabetic foot ulcers. A total of 42 patients with diabetic foot ulcers at our hospital from January 2017 to July 2020 were retrospectively analyzed. A control group and a PRF group were established. The two groups of patients underwent debridement. In the platelet-rich fibrin (PRF) group, autologous L-PRF was used to cover ulcer wounds. One time each week, Vaseline gauze was used to cover the ulcer wounds. In contrast, the control group was treated with the external application of mupirocin ointment and recombinant human epidermal growth factor gel (yeast). Two times each week, the sterile Vaseline gauze was covered with a bandage. Both groups were treated for 5 weeks. The wound recovery of the two groups was observed. During the early stage of treatment (first and second weeks) for diabetic foot ulcers, the wound healing rate was significantly better with L-PRF treatment than traditional treatment. For later-stage treatment (third to fifth weeks), the overall cure rate was higher with L-PRF than the traditional treatment method. L-PRF can effectively promote wound healing in diabetic foot ulcers.

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