Bioethanol production: An integrated process of low substrate loading hydrolysis-high sugars liquid fermentation and solid state fermentation of enzymatic hydrolysis residue

Solar-energy driven solid-state fermentation was developed for continuous bioethanol production from glucose. Bioethanol was tested in alkaline-acid direct ethanol fuel cells for its potential as fuel.

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Amylase Production from Solid State Fermentation and Submerged Liquid Fermentation by Aspergillus niger

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v47i1.7310 ◽

2012 ◽

Vol 47 (1) ◽

pp. 99-104 ◽

Cited By ~ 3

Author(s):

G Dharani ◽

NS Kumaran

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Solid State Fermentation ◽

Specific Activity ◽

Basal Medium ◽

Banana Peel ◽

Liquid Fermentation ◽

Cultural Conditions ◽

Solid Substrates ◽

Moisture Conditions

The purpose of this work is to study the optimized cultural conditions for the production of amylase by Aspergillus niger in solid state and submerged liquid fermentation. Four solid substrates banana peel, corn, potato and tapioca with different moisture conditions were taken for solid state fermentation (SSF). Basal medium was used for submerged liquid fermentation (SLF) with different pH (3 to 8), temperature (25, 30, 35 and 40ºC), carbon concentration (1, 2 and 3 g) and nitrogen source (0.1, 0.2 and 0.3 g). In SSF, tapioca yielded highest amylase activity and specific activity (4.43U/ml and 4.58U/mg) at 50% moisture content. In SLF, 2 g starch and 0.3 g peptone concentration showed 0.78 and 1.23 U/ml amylase activities under the optimum pH (5) and temperature (30ºC) the amylase activities reached to 0.86 U/ml and 0.76 U/ml respectively. In SSF using tapioca as substrate the enzyme yield is about five times higher than that achieved with submerged liquid culture. DOI: http://dx.doi.org/10.3329/bjsir.v47i1.7310 Bangladesh J. Sci. Ind. Res. 47(1), 99-104, 2012

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Feasibility Study on Bioethanol Production by One Phase Transition Separation Based on Advanced Solid-State Fermentation

Energies ◽

10.3390/en14196301 ◽

2021 ◽

Vol 14 (19) ◽

pp. 6301

Author(s):

Hongshen Li ◽

Hongrui Liu ◽

Shizhong Li

Keyword(s):

Phase Transition ◽

Energy Consumption ◽

Solid State ◽

Solid State Fermentation ◽

Low Cost ◽

Cost Effective ◽

Fuel Ethanol ◽

Bioethanol Production ◽

Whole Process ◽

Vapor Permeation

To fulfill the consumption demand of low-cost fuel ethanol, an advanced process for feedstock fermentation and bioethanol extraction was required. This study proposed a process of combined continuous solid-state distillation and vapor permeation to extract ethanol from fermented sweet sorghum bagasse on the basis of advanced solid-state fermentation technology. Ethanol undergoes only one phase transition separation in the whole process, which drastically reduces energy consumption compared to the repeating phase transitions that occur in conventional bioethanol production. The mass balance and energy consumption of combining processes were simulated overall. A techno-economic evaluation was conducted on the flowsheet. Costs and profit of fuel ethanol produced by one phase transition separation bioethanol-producing technology were comprehensively calculated. The results of the present study show that the proposed process is an energy efficient and cost-effective alternative to conventional bioethanol production.

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Production of cellulases by solid state fermentation with Aspergillus terreus and enzymatic hydrolysis of mild alkali-treated rice straw

Bioresource Technology ◽

10.1016/j.biortech.2012.05.140 ◽

2012 ◽

Vol 121 ◽

pp. 355-361 ◽

Cited By ~ 79

Author(s):

Madhuri Narra ◽

Garima Dixit ◽

Jyoti Divecha ◽

Datta Madamwar ◽

Amita R. Shah

Keyword(s):

Solid State ◽

Enzymatic Hydrolysis ◽

Rice Straw ◽

Solid State Fermentation ◽

Aspergillus Terreus ◽

Hydrolysis Of

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Cellulases production on paper and sawdust using native Trichoderma asperellum

Universitas Scientiarum ◽

10.11144/javeriana.sc23-3.cpop ◽

2018 ◽

Vol 23 (3) ◽

pp. 419-436

Author(s):

Y Maritza Zapata ◽

Angelica Galviz-Quezada ◽

Víctor Manuel Osorio Echeverri

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Second Generation ◽

Lignocellulosic Material ◽

Trichoderma Asperellum ◽

Bioethanol Production ◽

Chemical Pretreatment ◽

Cellulosic Materials ◽

Second Generation Biofuels ◽

Glycosidic Bonds

Microbial cellulases are industrially used enzymes that catalyze the cleavage of the glycosidic bonds of cellulose. This hydrolysis yields sugars that can be used in processes such as bioethanol production. These enzymes are mainly produced by fungi belonging to the genus Trichoderma via submerged or solid state fermentation with cellulosic materials as substrates. Recent publications have increasingly demonstrated that alternatives to T.reesei enzymes in the production of second generation biofuels exist. Here, cellulolytic activities of crude extracts obtained from a native isolate of T.asperellum from coffe pulp and a strain of T.reesei were evaluated. Solid state fermentations were performed using paper and sawdust as substrates. The activities were measured after 12 days of incubation. The extracts obtained from T.reesei showed higher cellulase and endoglucanase activities (6.5 and 5.8 U/g) than those obtained using T.asperellum (5.6 and 4.1 U/g) with paper as substrate. There were no significant differences between isolates when grownon sawdust. It was possible to verify that native T.asperellum was able to produce cellulases on lignocellulosic material such as moistened paper and sawdust without having undergone a chemical pretreatment.

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Characterization of best naringinase producing fungus strain isolated from palmyrah (Borrasus flabellifer) fruit pulp

International Journal of Biological Research ◽

10.14419/ijbr.v4i2.6289 ◽

2016 ◽

Vol 4 (2) ◽

pp. 97

Author(s):

Sinthuja Karuppaija ◽

Kapilan Ranganathan ◽

Vasantharuba Seevaratnam

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Large Scale ◽

Agricultural Waste ◽

Selective Medium ◽

Fruit Pulp ◽

Fermentation System ◽

Fungal Strains ◽

Liquid Fermentation ◽

Fermentation Media

Background: The Palmyrah (Borrasus flabellifer L.) fruit pulp has the bitter compound flabelliferin (a tetraglycoside) which can be hydrolyzed by naringinase enzyme. The diverse groups of filamentous fungi and bacteria that live in different substrates have the capacity of producing extracellular naringinase enzyme which is of tremendous industrial value.Objective: The objective of the study was to isolate the naringinase producing fungal strains from Palmyrah and to identify the best naringinase producer under liquid and solid state fermentation systems.Methods: Fungal strains isolated from Palmyrah fruit pulp and the soil where pulp is allowed to decay, were grown on naringin agar selective medium at pH 6.0 at room temperature and the production of extracellular naringinase was measured in the liquid fermentation media and solid state fermentation system using paddy husk as support.Results: Five fungal strains isolated from the palmyrah pulp and the pulp decaying in sand designated as PF1,PF2,PF3,PF4 & PF5 had the ability to produce extracellular naringinase enzyme in liquid fermentation media. Fungal strain PF4 that showed highest naringinase enzyme activity (1.769U/ml) was selected among the isolated five fungal strains and identified as Rhizophus stolonifer based on the morphological and biochemical characteristics. When this strain was grown in the solid state fermentation system using paddy husk as media, narininase production was higher (269.84 U/gram of dry substrate) in seven days.Conclusion: Rhizophus stolonifer could be used to produce large scale naringinase enzyme under solid state fermentation system using very cheap, easily available, agricultural waste paddy husk as support without the need of expensive and well equipped laboratories.

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