n-Butanol production from lignocellulosic biomass hydrolysates without detoxification by Clostridium tyrobutyricum Δack-adhE2 in a fibrous-bed bioreactor

2019 ◽  
Vol 289 ◽  
pp. 121749 ◽  
Author(s):  
Jing Li ◽  
Yinming Du ◽  
Teng Bao ◽  
Jie Dong ◽  
Meng Lin ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Clostridium Tyrobutyricum ◽  
Butanol Production ◽  
Fibrous Bed Bioreactor ◽  
Biomass Hydrolysates

Butyric acid from anaerobic fermentation of lignocellulosic biomass hydrolysates by Clostridium tyrobutyricum strain RPT-4213

2013 ◽  
Vol 143 ◽  
pp. 322-329 ◽  
Author(s):  
Siqing Liu ◽  
Kenneth M. Bischoff ◽  
Timothy D. Leathers ◽  
Nasib Qureshi ◽  
Joseph O. Rich ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Butyric Acid ◽  
Anaerobic Fermentation ◽  
Clostridium Tyrobutyricum ◽  
Biomass Hydrolysates

scholarly journals Engineering the Oleaginous Yeast Rhodosporidium toruloides for Improved Resistance Against Inhibitors in Biomass Hydrolysates

2021 ◽  
Vol 9 ◽  
Author(s):  
Liting Lyu ◽  
Yadong Chu ◽  
Sufang Zhang ◽  
Yue Zhang ◽  
Qitian Huang ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Cell Mass ◽  
Peroxidase Gene ◽  
The Past ◽  
Yeast Strains ◽  
Biomass Hydrolysates ◽  
Improved Performance ◽  
Inhibitor Resistance

Conversion of lignocellulosic biomass into lipids and related chemicals has attracted much attention in the past two decades, and the oleaginous yeast Rhodosporidiumtoruloides has been widely used in this area. While R. toruloides species naturally have physiological advantages in terms of substrate utilization, lipid accumulation, and inhibitor resistance, reduced lipid production and cell growth are noticed when biomass hydrolysates are used as feedstocks. To improve the robustness of R. toruloides, here, we devised engineered strains by overexpressing genes responsible for phenolic compound degradation. Specifically, gene expression cassettes of the manganese peroxidase gene (MNP) and versatile peroxidase gene (VP) were constructed and integrated into the genome of R. toruloides NP11. A series of engineered strains were evaluated for lipid production in the presence of typical phenolic inhibitors. The results showed that R. toruloides strains with proper expression of MNP or VP indeed grew faster in the presence of vanillin and 5-hydroxymethylfurfural than the parental strain. When cultivated in concentrated mode biomass hydrolysates, the strain VP18 had improved performance as the cell mass and lipid content increased by 30% and 25%, respectively. This study provides more robust oleaginous yeast strains for microbial lipid production from lignocellulosic biomass, and similar efforts may be used to devise more advanced lipid producers.

Consolidated bioprocessing performance of a two‐species microbial consortium for butanol production from lignocellulosic biomass

2020 ◽  
Vol 117 (10) ◽  
pp. 2985-2995 ◽  
Author(s):  
Yujia Jiang ◽  
Yang Lv ◽  
Ruofan Wu ◽  
Jiasheng Lu ◽  
Weiliang Dong ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Microbial Consortium ◽  
Consolidated Bioprocessing ◽  
Butanol Production

scholarly journals A novel AST2 mutation generated upon whole-genome transformation of Saccharomyces cerevisiae confers high tolerance to 5-Hydroxymethylfurfural (HMF) and other inhibitors

PLoS Genetics ◽  
2021 ◽  
Vol 17 (10) ◽  
pp. e1009826
Author(s):  
Gert Vanmarcke ◽  
Quinten Deparis ◽  
Ward Vanthienen ◽  
Arne Peetermans ◽  
Maria R. Foulquié-Moreno ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lignocellulosic Biomass ◽  
Causative Mutation ◽  
Small Scale ◽  
Whole Genome ◽  
Biomass Hydrolysis ◽  
Inhibitor Tolerance ◽  
Cell Factories ◽  
Biomass Hydrolysates

Development of cell factories for conversion of lignocellulosic biomass hydrolysates into biofuels or bio-based chemicals faces major challenges, including the presence of inhibitory chemicals derived from biomass hydrolysis or pretreatment. Extensive screening of 2526 Saccharomyces cerevisiae strains and 17 non-conventional yeast species identified a Candida glabrata strain as the most 5-hydroxymethylfurfural (HMF) tolerant. Whole-genome (WG) transformation of the second-generation industrial S. cerevisiae strain MD4 with genomic DNA from C. glabrata, but not from non-tolerant strains, allowed selection of stable transformants in the presence of HMF. Transformant GVM0 showed the highest HMF tolerance for growth on plates and in small-scale fermentations. Comparison of the WG sequence of MD4 and GVM1, a diploid segregant of GVM0 with similarly high HMF tolerance, surprisingly revealed only nine non-synonymous SNPs, of which none were present in the C. glabrata genome. Reciprocal hemizygosity analysis in diploid strain GVM1 revealed AST2N406I as the only causative mutation. This novel SNP improved tolerance to HMF, furfural and other inhibitors, when introduced in different yeast genetic backgrounds and both in synthetic media and lignocellulose hydrolysates. It stimulated disappearance of HMF and furfural from the medium and enhanced in vitro furfural NADH-dependent reducing activity. The corresponding mutation present in AST1 (i.e. AST1D405I) the paralog gene of AST2, also improved inhibitor tolerance but only in combination with AST2N406I and in presence of high inhibitor concentrations. Our work provides a powerful genetic tool to improve yeast inhibitor tolerance in lignocellulosic biomass hydrolysates and other inhibitor-rich industrial media, and it has revealed for the first time a clear function for Ast2 and Ast1 in inhibitor tolerance.

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