Enhancing microbial lipids yield for biodiesel production by oleaginous yeast Lipomyces starkeyi fermentation: A review

2021 ◽  
pp. 126294
Author(s):  
Le Zhang ◽  
Jonathan T.E. Lee ◽  
Yong Sik Ok ◽  
Yanjun Dai ◽  
Yen Wah Tong Resource
Keyword(s):  
Oleaginous Yeast ◽  
Biodiesel Production ◽  
Microbial Lipids ◽  
Lipomyces Starkeyi

scholarly journals Citrate-Mediated Acyl-CoA Synthesis Is Required for the Promotion of Growth and Triacylglycerol Production in Oleaginous Yeast Lipomyces starkeyi

2021 ◽  
Vol 9 (8) ◽  
pp. 1693
Author(s):  
Rikako Sato ◽  
Satoshi Ara ◽  
Harutake Yamazaki ◽  
Koji Ishiya ◽  
Sachiyo Aburatani ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Gradient Centrifugation ◽  
Screening Method ◽  
Lipid Productivity ◽  
Biodiesel Production ◽  
Lipomyces Starkeyi ◽  
Atp Citrate Lyase ◽  
Percoll Density Gradient Centrifugation ◽  
Citrate Lyase ◽  
Percoll Density Gradient

The oleaginous yeast Lipomyces starkeyi is an excellent producer of triacylglycerol (TAG) as a feedstock for biodiesel production. To understand the regulation of TAG synthesis, we attempted to isolate mutants with decreased lipid productivity and analyze the expression of TAG synthesis-related genes in this study. A mutant with greatly decreased lipid productivity, sr22, was obtained by an effective screening method using Percoll density gradient centrifugation. The expression of citrate-mediated acyl-CoA synthesis-related genes (ACL1, ACL2, ACC1, FAS1, and FAS2) was decreased in the sr22 mutant compared with that of the wild-type strain. Together with a notion that L. starkeyi mutants with increased lipid productivities had increased gene expression, there was a correlation between the expression of these genes and TAG synthesis. To clarify the importance of citrate-mediated acyl-CoA synthesis pathway on TAG synthesis, we also constructed a strain with no ATP-citrate lyase responsible for the first reaction of citrate-mediated acyl-CoA synthesis and investigated the importance of ATP-citrate lyase on TAG synthesis. The ATP-citrate lyase was required for the promotion of cell growth and TAG synthesis in a glucose medium. This study may provide opportunities for the development of an efficient TAG synthesis for biodiesel production.

Genetically Engineered Oleaginous Yeast Lipomyces starkeyi for Sesquiterpene α-Zingiberene Production

2021 ◽  
Author(s):  
Ziyu Dai ◽  
Kyle R. Pomraning ◽  
Ellen A. Panisko ◽  
Beth A. Hofstad ◽  
Kristen B. Campbell ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Genetically Engineered ◽  
Lipomyces Starkeyi

Enhanced production of microbial lipids from waste office paper by the oleaginous yeast Cryptococcus curvatus

Fuel ◽  
2018 ◽  
Vol 217 ◽  
pp. 420-426 ◽  
Author(s):  
Neelamegam Annamalai ◽  
Nallusamy Sivakumar ◽  
Piotr Oleskowicz-Popiel
Keyword(s):  
Oleaginous Yeast ◽  
Microbial Lipids ◽  
Cryptococcus Curvatus ◽  
Enhanced Production ◽  
Waste Office Paper ◽  
Office Paper
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