Poly(dimethyl siloxane)-based protein chip for simultaneous detection of multiple samples: Use of glycidyl methacrylate photopolymer for site-specific protein immobilization

2006 ◽  
Vol 22 (5) ◽  
pp. 613-620 ◽  
Author(s):  
Kyoung Hwan Park ◽  
Hyun Gyu Park ◽  
Joon-Ho Kim ◽  
Ki Hun Seong
Keyword(s):  
Glycidyl Methacrylate ◽  
Simultaneous Detection ◽  
Protein Immobilization ◽  
Specific Protein ◽  
Protein Chip ◽  
Site Specific ◽  
Dimethyl Siloxane ◽  
Multiple Samples

Site-Specific Protein Immobilization by Staudinger Ligation

2003 ◽  
Vol 125 (39) ◽  
pp. 11790-11791 ◽  
Author(s):  
Matthew B. Soellner ◽  
Kimberly A. Dickson ◽  
Bradley L. Nilsson ◽  
Ronald T. Raines
Keyword(s):  
Protein Immobilization ◽  
Specific Protein ◽  
Staudinger Ligation ◽  
Site Specific

Site-Specific Protein Immobilization Using Unnatural Amino Acids

2014 ◽  
Vol 25 (11) ◽  
pp. 1916-1920 ◽  
Author(s):  
Benjamin K. Raliski ◽  
Christina A. Howard ◽  
Douglas D. Young
Keyword(s):  
Amino Acids ◽  
Unnatural Amino Acids ◽  
Protein Immobilization ◽  
Specific Protein ◽  
Site Specific

Site-specific protein immobilization through N-terminal oxime linkages

2007 ◽  
Vol 17 (19) ◽  
pp. 2021 ◽  
Author(s):  
Karen L. Christman ◽  
Rebecca M. Broyer ◽  
Zachary P. Tolstyka ◽  
Heather D. Maynard
Keyword(s):  
Protein Immobilization ◽  
Specific Protein ◽  
Site Specific

Site specific protein immobilization into structured polymer brushes prepared by AFM lithography

Soft Matter ◽  
2011 ◽  
Vol 7 (21) ◽  
pp. 9854 ◽  
Author(s):  
Hendrik Wagner ◽  
Yong Li ◽  
Michael Hirtz ◽  
Lifeng Chi ◽  
Harald Fuchs ◽  
...  
Keyword(s):  
Polymer Brushes ◽  
Protein Immobilization ◽  
Specific Protein ◽  
Site Specific ◽  
Afm Lithography

Biofunctionalization of carbon nanotubes for reversible site-specific protein immobilization

2021 ◽  
Vol 129 (9) ◽  
pp. 094302
Author(s):  
Lara Jorde ◽  
Zehao Li ◽  
Adrian Pöppelwerth ◽  
Jacob Piehler ◽  
Changjiang You ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Protein Immobilization ◽  
Specific Protein ◽  
Site Specific

scholarly journals Discovery and Interrogation of Functional Protein Modifications by Hotspot Thermal Profiling

2019 ◽  
Author(s):  
Jun X. Huang ◽  
Gihoon Lee ◽  
Raymond E. Moellering
Keyword(s):  
Posttranslational Modifications ◽  
Protein Function ◽  
Cell Types ◽  
Specific Protein ◽  
Live Cells ◽  
Functional Protein ◽  
Site Specific ◽  
Native Proteins ◽  
Thermal Profiling ◽  
Multiple Samples

Abstract Hundreds of protein posttranslational modification types have been reported across diverse organisms, however we still lack methods to systematically predict, or even prioritize, which modification sites may perturb protein function under specific cellular contexts. This protocol describes a method to detect the effects of site-specific protein phosphorylation on the thermal stability of thousands of native proteins in live cells. This mass spectrometry-based protocol measures shifts in overall protein stability in response to site-specific phosphorylation sites. The resulting dataset can enable discovery of intrinsic changes to protein structure as well as extrinsic changes to protein-protein, and protein-metabolite interactions, and can help prioritize site-specific study in a high-throughput and unbiased fashion. This approach takes several days complete, can be performed with multiple samples in parallel and is applicable to diverse organisms, cell types and posttranslational modifications.

Site-specific protein immobilization in a microfluidic chip channelvia an IEF-gelation process

2007 ◽  
Vol 28 (10) ◽  
pp. 1587-1594 ◽  
Author(s):  
Mianhong Shi ◽  
Youyuan Peng ◽  
Shaoning Yu ◽  
Baohong Liu ◽  
Jilie Kong
Keyword(s):  
Microfluidic Chip ◽  
Protein Immobilization ◽  
Specific Protein ◽  
Site Specific ◽  
Gelation Process

General Method for Site-Specific Protein Immobilization by Staudinger Ligation

2007 ◽  
Vol 18 (4) ◽  
pp. 1064-1069 ◽  
Author(s):  
Jeet Kalia ◽  
Nicholas L. Abbott ◽  
Ronald T. Raines
Keyword(s):  
Protein Immobilization ◽  
Specific Protein ◽  
Staudinger Ligation ◽  
Site Specific ◽  
General Method
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