RNAi gene silencing using cerasome as a viral-size siRNA-carrier free from fusion and cross-linking

2007 ◽  
Vol 17 (14) ◽  
pp. 3935-3938 ◽  
Author(s):  
Kazuki Matsui ◽  
Yoshihiro Sasaki ◽  
Takayoshi Komatsu ◽  
Masaru Mukai ◽  
Jun-ichi Kikuchi ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cross Linking ◽  
Carrier Free

Cationic siRNAs Provide Carrier-Free Gene Silencing in Animal Cells

2009 ◽  
Vol 131 (49) ◽  
pp. 17730-17731 ◽  
Author(s):  
Marc Nothisen ◽  
Mitsuharu Kotera ◽  
Emilie Voirin ◽  
Jean-Serge Remy ◽  
Jean-Paul Behr
Keyword(s):  
Gene Silencing ◽  
Animal Cells ◽  
Carrier Free ◽  
Free Gene

Preparation and Properties of Cross-Linked Enzyme Aggregates of Cellulase

2012 ◽  
Vol 581-582 ◽  
pp. 257-260 ◽  
Author(s):  
Bing Li ◽  
Shou Li Dong ◽  
Xiao Ling Xie ◽  
Zhen Bo Xu ◽  
Lin Li
Keyword(s):  
Enzyme Activity ◽  
Cross Linking ◽  
Optimal Temperature ◽  
Carrier Free ◽  
The Cross

A new carrier-free cross-linked aggregates of cellulase (CLEAs-C) via precipitation with ammonia sulfate and cross-linking with glutaraldehyde was prepared. Efficient enzyme activity was obtained when cellulase and glutaraldehyde concentration was 50mg/mL and 3% (v/v) respectively. The cross-linking time and temperature were also important parameters for immobilization. Optimal temperature and pH of the CLEA–C were evaluated. The CLEAs-C displayed good stabilities, after stored at 4○C for 28 days storage, the CLEAs retained more than 80% initial activities.

Structure Tuning of Cationic Oligospermine–siRNA Conjugates for Carrier-Free Gene Silencing

2016 ◽  
Vol 13 (8) ◽  
pp. 2718-2728 ◽  
Author(s):  
Marc Nothisen ◽  
Jérémy Bagilet ◽  
Jean-Paul Behr ◽  
Jean-Serge Remy ◽  
Mitsuharu Kotera
Keyword(s):  
Gene Silencing ◽  
Carrier Free ◽  
Free Gene

scholarly journals Carrier-free cellular uptake and the gene-silencing activity of the lipophilic siRNAs is strongly affected by the length of the linker between siRNA and lipophilic group

2011 ◽  
Vol 40 (5) ◽  
pp. 2330-2344 ◽  
Author(s):  
Natalya S. Petrova ◽  
Ivan V. Chernikov ◽  
Mariya I. Meschaninova ◽  
IIya S. Dovydenko ◽  
Aliya G. Venyaminova ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cellular Uptake ◽  
Carrier Free

scholarly journals Carrier-Free Immobilization of α-Galactosidase as Nano-Biocatalysts for Synthesizing Prebiotic α-Galacto-Oligosaccharides

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1248
Author(s):  
Yan Liu ◽  
Jingyi Yang ◽  
Ke Wang ◽  
Feiyu Duan ◽  
Lili Lu
Keyword(s):  
Ammonium Sulfate ◽  
Industrial Applications ◽  
Solvent Tolerance ◽  
Cross Linking ◽  
Organic Solvent Tolerance ◽  
Regular Structures ◽  
Carrier Free ◽  
High Precipitation ◽  
First Time ◽  
Scanning Electron

α-Galacto-oligosaccharides (α-GOSs) have great functions as prebiotics and therapeutics. This work established the method of batch synthesis of α-GOSs by immobilized α-galactosidase for the first time, laying a foundation for industrial applications in the future. The α-galactosidase from Aspergillus niger L63 was immobilized as cross-linked enzyme aggregates (CLEAs) nano-biocatalyst through enzyme precipitating and cross-linking steps without using carriers. Among the tested agents, the ammonium sulfate showed high precipitation efficacy and induced regular structures of α-galactosidase CLEAs (Aga-CLEAs) that had been analyzed by scanning electron microscopy and Fourier-transform infrared spectroscopy. Through optimization by response surface methodology, the ammonium sulfate-induced Aga-CLEAs achieved a high activity recovery of around 90% at 0.55 U/mL of enzymes and 36.43 mM glutaraldehyde with cross-linking for 1.71 h. Aga-CLEAs showed increased thermal stability and organic solvent tolerance. The storage ability was also improved since it maintained 74.5% activity after storing at 4 °C for three months, significantly higher than that of the free enzyme (21.6%). Moreover, Aga-CLEAs exhibited excellent reusability in the α-GOSs synthesis from galactose, retaining above 66% of enzyme activity after 10 batch reactions, with product yields all above 30%.

scholarly journals Effective carrier-free gene-silencing activity of cholesterol-modified siRNAs

RSC Advances ◽  
2018 ◽  
Vol 8 (41) ◽  
pp. 22963-22966 ◽  
Author(s):  
Lidya Salim ◽  
Chris McKim ◽  
Jean-Paul Desaulniers
Keyword(s):  
Gene Silencing ◽  
Great Promise ◽  
Chemical Modifications ◽  
Pharmacokinetic Properties ◽  
Carrier Free ◽  
Free Gene ◽  
Effective Carrier

The use of short interfering RNAs (siRNAs) as therapeutics holds great promise, but chemical modifications must first be employed to improve their pharmacokinetic properties.

Carrier-Free Immobilization of Lipase from Candida rugosa with Polyethyleneimines by Carboxyl-Activated Cross-Linking

2014 ◽  
Vol 15 (5) ◽  
pp. 1896-1903 ◽  
Author(s):  
Susana Velasco-Lozano ◽  
Fernando López-Gallego ◽  
Rafael Vázquez-Duhalt ◽  
Juan C. Mateos-Díaz ◽  
José M. Guisán ◽  
...  
Keyword(s):  
Candida Rugosa ◽  
Cross Linking ◽  
Carrier Free ◽  
Lipase From Candida Rugosa

scholarly journals Carrier-free Gene Silencing by Amphiphilic Nucleic Acid Conjugates in Differentiated Intestinal Cells

2016 ◽  
Vol 5 ◽  
pp. e364 ◽  
Author(s):  
Elena Moroz ◽  
Soo Hyeon Lee ◽  
Ken Yamada ◽  
François Halloy ◽  
Saúl Martínez-Montero ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Gene Silencing ◽  
Intestinal Cells ◽  
Carrier Free ◽  
Free Gene

scholarly journals Lipophilic siRNA targets albumin in situ and promotes bioavailability, tumor penetration, and carrier-free gene silencing

2017 ◽  
Vol 114 (32) ◽  
pp. E6490-E6497 ◽  
Author(s):  
Samantha M. Sarett ◽  
Thomas A. Werfel ◽  
Linus Lee ◽  
Meredith A. Jackson ◽  
Kameron V. Kilchrist ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Xenograft Model ◽  
Fold Increase ◽  
Cell Uptake ◽  
Orthotopic Model ◽  
Pharmacokinetic Properties ◽  
Carrier Free ◽  
Tumor Accumulation

Clinical translation of therapies based on small interfering RNA (siRNA) is hampered by siRNA's comprehensively poor pharmacokinetic properties, which necessitate molecule modifications and complex delivery strategies. We sought an alternative approach to commonly used nanoparticle carriers by leveraging the long-lived endogenous serum protein albumin as an siRNA carrier. We synthesized siRNA conjugated to a diacyl lipid moiety (siRNA-L2), which rapidly binds albumin in situ. siRNA-L2, in comparison with unmodified siRNA, exhibited a 5.7-fold increase in circulation half-life, an 8.6-fold increase in bioavailability, and reduced renal accumulation. Benchmarked against leading commercial siRNA nanocarrier in vivo jetPEI, siRNA-L2 achieved 19-fold greater tumor accumulation and 46-fold increase in per-tumor-cell uptake in a mouse orthotopic model of human triple-negative breast cancer. siRNA-L2 penetrated tumor tissue rapidly and homogeneously; 30 min after i.v. injection, siRNA-L2 achieved uptake in 99% of tumor cells, compared with 60% for jetPEI. Remarkably, siRNA-L2 achieved a tumor:liver accumulation ratio >40:1 vs. <3:1 for jetPEI. The improved pharmacokinetic properties of siRNA-L2 facilitated significant tumor gene silencing for 7 d after two i.v. doses. Proof-of-concept was extended to a patient-derived xenograft model, in which jetPEI tumor accumulation was reduced fourfold relative to the same formulation in the orthotopic model. The siRNA-L2 tumor accumulation diminished only twofold, suggesting that the superior tumor distribution of the conjugate over nanoparticles will be accentuated in clinical situations. These data reveal the immense promise of in situ albumin targeting for development of translational, carrier-free RNAi-based cancer therapies.

Influence of Calcium Ions on the Plant Cell Surface: Membrane Fusions and Conformational Changes

1974 ◽  
Vol 32 ◽  
pp. 154-155
Author(s):  
D. James Morré ◽  
Charles E. Bracker ◽  
William J. VanDerWoude
Keyword(s):  
Cell Wall ◽  
Cell Surface ◽  
Conformational Changes ◽  
Calcium Ions ◽  
Surface Membrane ◽  
Carboxyl Groups ◽  
Cross Linking ◽  
Ultrastructural Evidence ◽  
Glycine Max L ◽  
Wall Extensibility

Calcium ions in the concentration range 5-100 mM inhibit auxin-induced cell elongation and wall extensibility of plant stems. Inhibition of wall extensibility requires that the tissue be living; growth inhibition cannot be explained on the basis of cross-linking of carboxyl groups of cell wall uronides by calcium ions. In this study, ultrastructural evidence was sought for an interaction of calcium ions with some component other than the wall at the cell surface of soybean (Glycine max (L.) Merr.) hypocotyls.

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