scholarly journals Correlation between Myofibrillar Biochemistry and Muscle Fiber Mechanics using Rabbit Psoas Muscle Preparations Indicates that Phase 2 of Step Analysis Represents the Cross-Bridge Detachment Step

2010 ◽  
Vol 98 (3) ◽  
pp. 144a-145a
Author(s):  
Robin Candau ◽  
Corinne Lionne ◽  
Tom Barman ◽  
Masataka Kawai
Keyword(s):  
Muscle Fiber ◽  
Psoas Muscle ◽  
Phase 2 ◽  
Rabbit Psoas ◽  
Cross Bridge ◽  
The Cross

scholarly journals Reversal of the cross-bridge force-generating transition by photogeneration of phosphate in rabbit psoas muscle fibres.

1992 ◽  
Vol 451 (1) ◽  
pp. 247-278 ◽  
Author(s):  
J A Dantzig ◽  
Y E Goldman ◽  
N C Millar ◽  
J Lacktis ◽  
E Homsher
Keyword(s):  
Psoas Muscle ◽  
Muscle Fibres ◽  
Rabbit Psoas ◽  
Cross Bridge ◽  
The Cross

BDM affects nucleotide binding and force generation steps of the cross-bridge cycle in rabbit psoas muscle fibers

1994 ◽  
Vol 266 (2) ◽  
pp. C437-C447 ◽  
Author(s):  
Y. Zhao ◽  
M. Kawai
Keyword(s):  
Equilibrium Constants ◽  
Muscle Fibers ◽  
Psoas Muscle ◽  
Isometric Tension ◽  
Activation Mechanism ◽  
Phosphate Binding ◽  
Rabbit Psoas ◽  
Cross Bridge ◽  
The Cross ◽  
Cross Bridges

The effect of 2,3-butanedione monoxime (BDM) on elementary steps of the cross-bridge cycle was studied with the sinusoidal analysis technique in skinned rabbit psoas muscle fibers. Our results showed that isometric tension and stiffness decreased progressively with an increase in the BDM concentration. The MgATP and MgADP binding constants increased 27 and 6 times, respectively, when BDM was increased from 0 to 18 mM, whereas the phosphate binding constant did not change significantly. The equilibrium constants of the ATP isomerization and detachment step were not sensitive to BDM, whereas the equilibrium constant of the attachment (power stroke) step decreased with BDM. Thus, in the presence of BDM, the number of attached cross bridges decreases; more cross bridges accumulate in the detached state, causing isometric tension and stiffness to decline. However, our detailed analysis shows that the decrease in the number of attached cross bridges is approximately 40%, which is not adequate to account for the 84% decrease in the isometric tension when 18 mM BDM was present. Therefore we suggest that a thin-filament activation mechanism is also affected by BDM.

scholarly journals Is the Cross-Bridge Stiffness Proportional to Tension during Muscle Fiber Activation?

2010 ◽  
Vol 98 (11) ◽  
pp. 2582-2590 ◽  
Author(s):  
Barbara Colombini ◽  
Marta Nocella ◽  
M. Angela Bagni ◽  
Peter J. Griffiths ◽  
Giovanni Cecchi
Keyword(s):  
Muscle Fiber ◽  
Cross Bridge ◽  
The Cross
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