scholarly journals Decoding Cytoskeleton-Anchored and Non-Anchored Receptors from Single-Cell Adhesion Force Data

2015 ◽  
Vol 109 (7) ◽  
pp. 1330-1333 ◽  
Author(s):  
Ediz Sariisik ◽  
Cvetan Popov ◽  
Jochen P. Müller ◽  
Denitsa Docheva ◽  
Hauke Clausen-Schaumann ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Adhesion Force ◽  
Force Data

scholarly journals Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Milan Sztilkovics ◽  
Tamas Gerecsei ◽  
Beatrix Peter ◽  
Andras Saftics ◽  
Sandor Kurunczi ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Temporal Resolution ◽  
Adhesion Force ◽  
Optical Biosensor ◽  
Cell Populations ◽  
Label Free ◽  
Kinetic Evaluation ◽  
Force Microscopy ◽  
Kinetics Of

AbstractSingle-cell adhesion force plays a crucial role in biological sciences, however its in-depth investigation is hindered by the extremely low throughput and the lack of temporal resolution of present techniques. While atomic force microcopy (AFM) based methods are capable of directly measuring the detachment force values between individual cells and a substrate, their throughput is limited to few cells per day, and cannot provide the kinetic evaluation of the adhesion force over the timescale of several hours. In this study a high spatial and temporal resolution resonant waveguide grating based label-free optical biosensor was combined with robotic fluidic force microscopy to monitor the adhesion of living cancer cells. In contrast to traditional fluidic force microscopy methods with a manipulation range in the order of 300–400 micrometers, the robotic device employed here can address single cells over mm-cm scale areas. This feature significantly increased measurement throughput, and opened the way to combine the technology with the employed microplate-based, large area biosensor. After calibrating the biosensor signals with the direct force measuring technology on 30 individual cells, the kinetic evaluation of the adhesion force and energy of large cell populations was performed for the first time. We concluded that the distribution of the single-cell adhesion force and energy can be fitted by log-normal functions as cells are spreading on the surface and revealed the dynamic changes in these distributions. The present methodology opens the way for the quantitative assessment of the kinetics of single-cell adhesion force and energy with an unprecedented throughput and time resolution, in a completely non-invasive manner.

scholarly journals Single-cell adhesion strength and contact density drops in the M phase of cancer cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rita Ungai-Salánki ◽  
Eleonóra Haty ◽  
Tamás Gerecsei ◽  
Barbara Francz ◽  
Bálint Béres ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Cancer Cells ◽  
Adhesion Strength ◽  
Adhesion Force ◽  
Single Cell Level ◽  
Cell Level ◽  
Contact Density ◽  
M Phase ◽  
Tissue Cells

AbstractThe high throughput, cost effective and sensitive quantification of cell adhesion strength at the single-cell level is still a challenging task. The adhesion force between tissue cells and their environment is crucial in all multicellular organisms. Integrins transmit force between the intracellular cytoskeleton and the extracellular matrix. This force is not only a mechanical interaction but a way of signal transduction as well. For instance, adhesion-dependent cells switch to an apoptotic mode in the lack of adhesion forces. Adhesion of tumor cells is a potential therapeutic target, as it is actively modulated during tissue invasion and cell release to the bloodstream resulting in metastasis. We investigated the integrin-mediated adhesion between cancer cells and their RGD (Arg-Gly-Asp) motif displaying biomimetic substratum using the HeLa cell line transfected by the Fucci fluorescent cell cycle reporter construct. We employed a computer-controlled micropipette and a high spatial resolution label-free resonant waveguide grating-based optical sensor calibrated to adhesion force and energy at the single-cell level. We found that the overall adhesion strength of single cancer cells is approximately constant in all phases except the mitotic (M) phase with a significantly lower adhesion. Single-cell evanescent field based biosensor measurements revealed that at the mitotic phase the cell material mass per unit area inside the cell-substratum contact zone is significantly less, too. Importantly, the weaker mitotic adhesion is not simply a direct consequence of the measured smaller contact area. Our results highlight these differences in the mitotic reticular adhesions and confirm that cell adhesion is a promising target of selective cancer drugs as the vast majority of normal, differentiated tissue cells do not enter the M phase and do not divide.

Single cell adhesion force measurement for cell viability identification using an AFM cantilever-based micro putter

2011 ◽  
Vol 22 (11) ◽  
pp. 115802 ◽  
Author(s):  
Yajing Shen ◽  
Masahiro Nakajima ◽  
Seiji Kojima ◽  
Michio Homma ◽  
Masaru Kojima ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Viability ◽  
Single Cell ◽  
Adhesion Force ◽  
Force Measurement ◽  
Afm Cantilever

scholarly journals A Review of Single-Cell Adhesion Force Kinetics and Applications

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 577
Author(s):  
Ashwini Shinde ◽  
Kavitha Illath ◽  
Pallavi Gupta ◽  
Pallavi Shinde ◽  
Ki-Taek Lim ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Developmental Disorders ◽  
Quantitative Methods ◽  
Adhesion Force ◽  
Force Measurement ◽  
Adhesive Force ◽  
A Cell ◽  
Sense And Respond ◽  
Cellular Deformability

Cells exert, sense, and respond to the different physical forces through diverse mechanisms and translating them into biochemical signals. The adhesion of cells is crucial in various developmental functions, such as to maintain tissue morphogenesis and homeostasis and activate critical signaling pathways regulating survival, migration, gene expression, and differentiation. More importantly, any mutations of adhesion receptors can lead to developmental disorders and diseases. Thus, it is essential to understand the regulation of cell adhesion during development and its contribution to various conditions with the help of quantitative methods. The techniques involved in offering different functionalities such as surface imaging to detect forces present at the cell-matrix and deliver quantitative parameters will help characterize the changes for various diseases. Here, we have briefly reviewed single-cell mechanical properties for mechanotransduction studies using standard and recently developed techniques. This is used to functionalize from the measurement of cellular deformability to the quantification of the interaction forces generated by a cell and exerted on its surroundings at single-cell with attachment and detachment events. The adhesive force measurement for single-cell microorganisms and single-molecules is emphasized as well. This focused review should be useful in laying out experiments which would bring the method to a broader range of research in the future.

scholarly journals Evaluation of Cell Adhesion Force with Atomic Force Microscope

2000 ◽  
Vol 40 (supplement) ◽  
pp. S80
Author(s):  
H. Kim ◽  
T. Osada ◽  
A. Ikai
Keyword(s):  
Cell Adhesion ◽  
Atomic Force Microscope ◽  
Adhesion Force ◽  
Atomic Force

Single Cell Adhesion in Cancer Progression

2021 ◽  
pp. 729-766
Author(s):  
Privita Edwina Rayappan George Edwin ◽  
Saumendra Bajpai
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Cancer Progression

Studies on Single-Cell Adhesion Probability between Lymphocytes and Endothelial Cells with Micropipette Technique

2002 ◽  
Vol 63 (2) ◽  
pp. 218-226 ◽  
Author(s):  
Hong Zhao ◽  
Xi Dong ◽  
Xiong Wang ◽  
Xiufeng Li ◽  
Fengyuan Zhuang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Adhesion ◽  
Single Cell ◽  
Adhesion Probability

scholarly journals Optical Single Cell Resolution Cytotoxicity Biosensor Based on Single Cell Adhesion Dot Arrays

2020 ◽  
Vol 92 (14) ◽  
pp. 9658-9665 ◽  
Author(s):  
Maite Garcia-Hernando ◽  
Alba Calatayud-Sanchez ◽  
Jaione Etxebarria-Elezgarai ◽  
Marian M. de Pancorbo ◽  
Fernando Benito-Lopez ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Single Cell
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