Assessment of 8-hydroxy-2-deoxyguanosine activity, gene expression and antioxidant enzyme activity on rainbow trout ( Oncorhynchus mykiss ) tissues exposed to biopesticide

2017 ◽  
Vol 203 ◽  
pp. 51-58 ◽  
Author(s):  
Gonca Alak ◽  
Arzu Ucar ◽  
Veysel Parlak ◽  
Aslı Çilingir Yeltekin ◽  
Ismail Hakkı Taş ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Antioxidant Enzyme ◽  
Antioxidant Enzyme Activity ◽  
Rainbow Trout Oncorhynchus Mykiss

Gene Expression and Enzyme Activity of Mitochondrial Proteins in Irradiated Rainbow Trout (Oncorhynchus Mykiss, Walbaum) TissuesIn Vitro

2009 ◽  
Vol 171 (4) ◽  
pp. 464-473 ◽  
Author(s):  
Colm O'Dowd ◽  
Carmel E. Mothersill ◽  
Michael T. Cairns ◽  
Brian Austin ◽  
Fiona M. Lyng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Mitochondrial Proteins ◽  
Rainbow Trout Oncorhynchus Mykiss

Rats fed a high sucrose diet have altered heart antioxidant enzyme activity and gene expression

Life Sciences ◽  
2002 ◽  
Vol 71 (11) ◽  
pp. 1303-1312 ◽  
Author(s):  
Jérôme Busserolles ◽  
Wioletta Zimowska ◽  
Edmond Rock ◽  
Yves Rayssiguier ◽  
Andrzej Mazur
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Antioxidant Enzyme Activity ◽  
Sucrose Diet ◽  
High Sucrose Diet ◽  
High Sucrose

Inter-tissue differences in mitochondrial enzyme activity, RNA and DNA in rainbow trout (Oncorhynchus mykiss)

1998 ◽  
Vol 201 (24) ◽  
pp. 3377-3384 ◽  
Author(s):  
S. C. Leary ◽  
B. J. Battersby ◽  
C. D. Moyes
Keyword(s):  
Enzyme Activity ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Citrate Synthase ◽  
Mitochondrial Protein ◽  
Molecular Organization ◽  
Mitochondrial Rna ◽  
Mitochondrial Enzyme ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
The Relationship

We examined whether the relationships between mitochondrial enzyme activity, mitochondrial DNA (mtDNA) and mitochondrial RNA (mtRNA) were conserved in rainbow trout (Oncorhynchus mykiss) tissues that differ widely in their metabolic and molecular organization. The activity of citrate synthase (CS), expressed either per gram of tissue or per milligram of total DNA, indicated that these tissues (blood, brain, kidney, liver,cardiac, red and white muscles) varied more than 100-fold in mitochondrial content. Several-fold differences in the levels of CS mRNA per milligram of DNA and CS activity per CS mRNA were also observed, suggesting that fundamental differences exist in the regulation of CS levels across tissues. Although tissues varied 14-fold in RNA g-1, poly(A+) RNA (mRNA)was approximately 2 % of total RNA in all tissues. DNA g-1 also varied 14-fold across tissues, but RNA:DNA ratios varied only 2.5-fold. The relationship between two mitochondrial mRNA species (COX I, ATPase VI) and one mitochondrial rRNA (16S) species was constant across tissues. The ratio of mtRNA to mtDNA was also preserved across most tissues; red and white muscle had 10- to 20-fold lower levels of mtDNA g-1 but 7- to 10-fold higher mtRNA:mtDNA ratios, respectively. Collectively, these data suggest that the relationship between mitochondrial parameters is highly conserved across most tissues, but that skeletal muscles differ in a number of important aspects of respiratory gene expression ('respiratory genes'include genes located on mtDNA and genes located in the nucleus that encode mitochondrial protein) and mtDNA transcriptional regulation.

Effects of high-soy diet on S100 gene expression in liver and intestine of rainbow trout (Oncorhynchus mykiss)

2019 ◽  
Vol 86 ◽  
pp. 764-771 ◽  
Author(s):  
Patrick C. Blaufuss ◽  
T. Gibson Gaylord ◽  
Wendy M. Sealey ◽  
Madison S. Powell
Keyword(s):  
Gene Expression ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
S100 Gene

scholarly journals Isolation and Identification Rust Pathogens and the Study of Antioxidant Enzyme Activity and Gene Expression under Rust Infection in Zoysia japonica

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1200
Author(s):  
Di Zhang ◽  
Hanguo Zeng ◽  
Liyuan Zhao ◽  
Jiaming Yue ◽  
Xiao Qi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Rust Resistance ◽  
Antioxidant Enzyme Activity ◽  
Zoysia Japonica ◽  
Rust Disease ◽  
Isolation And Identification ◽  
Rust Pathogens ◽  
Gene Expression Levels

The goal of this study was to identify the zoysiagrass rust pathogens and to analyze the differences in rust-resistant and rust-susceptible Zoysia japonica germplasm upon inoculation. Based on the assessment of spore morphology and 18S ribosomal DNA (rDNA) molecular identification, the zoysiagrass rust pathogen was identified as Puccinia zoysiae Diet. The development of mycelium, the rate of spreading, and the timing of spore production were more delayed in the rust-resistant (RR) genotype than the rust-susceptible (RS) genotype. After inoculation, the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) initially decreased, then increased in both the RR and RS genotypes, but the increased enzyme activities were faster in the RR than in the RS genotype. Rust resistance was positively correlated with antioxidant enzyme activity. The observed changes in CAT, POD and APX activity corresponded to their gene expression levels. The results of this study may be utilized in accurately evaluating the damage of rust disease and rust-resistance in zoysiagrass germplasm aimed at breeding the rust-resistant zoysiagrass varieties and improving disease management of zoysiagrass turf.

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