Interference of deferasirox with assays for serum iron and serum unsaturated iron binding capacity during iron chelating therapy

Abstract Ferritin values for 250 selected sera were compared with values for iron, total iron-binding capacity (TIBC), and transferrin saturation, to assess the potential of the ferritin assay for the detection of latent iron deficiency. The specimens were grouped (50 in each group) according to their values for iron and TIBC. In Group 1 (low iron, high TIBC) the saturation and ferritin values both indicated iron deficiency in all but one. In the 100 specimens of Groups 2 (normal iron, high TIBC) and 4 (normal iron, high normal TIBC), the saturation values revealed 16 iron-deficient cases, the ferritin test 55. For Groups 3 (low iron, normal TIBC) and 5 (low iron, low TIBC), the ferritin test revealed fewer cases of iron deficiency than did the saturation values (37 cases vs 51 cases, in the 100 specimens). Evidently the ferritin test detects iron deficiency in many cases for whom the serum iron and TIBC tests are not positively indicative. The correlation of serum ferritin with iron, TIBC, and transferrin saturation in the five groups was good only in the case of specimens for which the TIBC was normal; if it was abnormal the correlation was very poor.

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Use of an Anion-Exchange Column to Determine Serum Iron-Binding Capacity

Clinical Chemistry ◽

10.1093/clinchem/17.9.941 ◽

1971 ◽

Vol 17 (9) ◽

pp. 941-947 ◽

Cited By ~ 3

Author(s):

H Peter Lehmann ◽

Alex Kaplan

Keyword(s):

Serum Iron ◽

Binding Capacity ◽

Ferric Citrate ◽

Iron Binding ◽

Resin Column ◽

Anion Exchange Column ◽

Excess Iron ◽

Colorimetric Measurement ◽

Color Reagent ◽

Iron Binding Capacity

Abstract A rapid procedure is described for determining total iron-binding capacity (TIBC) by use of a small, disposable resin column. Serum is saturated with excess ferric citrate, and iron not bound to protein is then removed by passing the mixture through the column of anion-exchanger. Transferrin-bound iron in the effluent, the TIBC, is determined colorimetrically (AutoAnalyzer), with 2,4,6-tripyridyl-1,3,5-triazine as color reagent. The percentage saturation with iron may be obtained from colorimetric measurement of original serum iron and the TIBC, or by radioactive counting if 59Fe is incorporated in the ferric citrate used to saturate the serum. Our method is compared with the MgCO3 precipitation technique for the removal of excess iron after saturation and to an ultrafiltration technique in which protein-bound iron is retained by a membrane and the filtrate analyzed for unbound iron, thus obviating absorbents in the determination of TIBC. Results from the resin-column and ultrafiltration procedures correlated well, whereas those from the MgCO3 method were consistently lower and more erratic.

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NEOPTERIN AND INTERFERON-GAMMA AS IMMUNE RESPONSE MARKERS IN BETATHALASSEMIA MAJOR PATIENTS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i9.26661 ◽

2018 ◽

Vol 11 (9) ◽

pp. 192

Author(s):

Shahad F Obeid ◽

Sanad B Al-a'araji ◽

Bassam F Matti ◽

Hayder Adnan Fawzi

Keyword(s):

Immune System ◽

Interferon Gamma ◽

Serum Iron ◽

Binding Capacity ◽

Iron Status ◽

System Response ◽

Iron Binding ◽

Ifn Γ ◽

Immune System Response ◽

Iron Binding Capacity

Objective: The assessment of neopterin and interferon-gamma (IFN-γ) levels as a part of immune system response about serum iron status in β-thalassemia (TM) major patients.Methods: Spectrophotometry applied for the estimation of iron status including serum iron level, total iron-binding capacity, and unsaturated iron-binding capacity. Enzyme-linked immunosorbent assay (ELISA) applied for the estimation of the serum cytokines included neopterin and IFN-γ also serum ferritin estimation by ELISA from 130 β-TM major patients where they divided according to serum ferritin level (< and ≥2500 ng/mL).Results: The neopterin and IFN-γ concentration showed significantly increased with direct correlation among TM patient group when compared to the normal healthy control group. However, there was no significant difference between different levels of serum ferritin.Conclusion: The increased serum level of neopterin and IFN-γ found in patients with β-TM may be due to the direct effect of iron on cellular immune cells beside of immune system response to the effect of iron toxicity on different body organs. There was a direct moderate relationship between neopterin and IFN-γ.

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Serum Copper, Serum Iron and Total Iron-Binding Capacity of Serum in Acute and Chronic Infections.

Acta Medica Scandinavica ◽

10.1111/j.0954-6820.1953.tb07024.x ◽

2009 ◽

Vol 145 (5) ◽

pp. 315-325 ◽

Cited By ~ 20

Author(s):

P. BRENDSTRUP.

Keyword(s):

Serum Iron ◽

Binding Capacity ◽

Serum Copper ◽

Total Iron ◽

Iron Binding ◽

Total Iron Binding Capacity ◽

Chronic Infections ◽

Iron Binding Capacity

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Studies on Congenital Hemolytic Syndromes. IV. Gastrointestinal Absorption of Iron

Blood ◽

10.1182/blood.v19.3.359.359 ◽

1962 ◽

Vol 19 (3) ◽

pp. 359-378 ◽

Cited By ~ 77

Author(s):

MARION E. ERLANDSON ◽

BEATRICE WALDEN ◽

GERTRUDE STERN ◽

MARGARET W. HILGARTNER ◽

JACQUELINE WEHMAN ◽

...

Keyword(s):

Sickle Cell ◽

Serum Iron ◽

Binding Capacity ◽

Specific Effect ◽

Thalassemia Major ◽

Gastrointestinal Absorption ◽

Iron Binding ◽

Normal Children ◽

Hemoglobin C ◽

Iron Binding Capacity

Abstract 1. Results of studies of gastrointestinal absorption of ferrous iron in normal children and those with heterozygous thalassemia were similar. 2. In one patient with absent erythropoiesis but severe anemia, no increase in the amount of iron absorbed was noted. 3. In sickle cell-hemoglobin C disease and hereditary spherocytosis having only slight anemia in the presence of increased erythropoiesis, normal amounts of iron were absorbed. 4. Patients with sickle cell anemia and thalassemia major in whom there was active erythropoiesis and marked anemia absorbed abnormally large amounts of iron. The amount absorbed by individuals with the latter disease could be reduced by administration of transfusions and concomitant suppression of erythropoiesis. 5. Usual values for serum iron and latent iron-binding capacity in several congenital hemolytic syndromes have been presented and their significance discussed. 6. No specific effect on absorption was noted by increased or reduced amounts of tissue or serum iron or by reduced or increased latent iron-binding protein.

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