scholarly journals Pasteurella multocida toxin (PMT) upregulates CTGF which leads to mTORC1 activation in Swiss 3T3 cells

2013 ◽  
Vol 25 (5) ◽  
pp. 1136-1148 ◽  
Author(s):  
Hammou Oubrahim ◽  
Allison Wong ◽  
Brenda A. Wilson ◽  
P. Boon Chock
Keyword(s):  
Pasteurella Multocida ◽  
3T3 Cells ◽  
Pasteurella Multocida Toxin ◽  
Swiss 3T3 ◽  
Mtorc1 Activation

scholarly journals Pasteurella multocida toxin, a potent mitogen, increases inositol 1,4,5-trisphosphate and mobilizes Ca2+ in Swiss 3T3 cells.

1991 ◽  
Vol 266 (8) ◽  
pp. 4840-4847 ◽  
Author(s):  
J M Staddon ◽  
C J Barker ◽  
A C Murphy ◽  
N Chanter ◽  
A J Lax ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
3T3 Cells ◽  
Toxin A ◽  
Inositol 1,4,5 Trisphosphate ◽  
Pasteurella Multocida Toxin ◽  
Swiss 3T3

scholarly journals Modification of Heterotrimeric G-Proteins in Swiss 3T3 Cells Stimulated with Pasteurella multocida Toxin

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e47188 ◽  
Author(s):  
Rebecca C. Babb ◽  
Karen A. Homer ◽  
Jon Robbins ◽  
Alistair J. Lax
Keyword(s):  
G Proteins ◽  
Pasteurella Multocida ◽  
Heterotrimeric G Proteins ◽  
3T3 Cells ◽  
Pasteurella Multocida Toxin ◽  
Swiss 3T3

scholarly journals Differential Modulation and Subsequent Blockade of Mitogenic Signaling and Cell Cycle Progression by Pasteurella multocida Toxin

2000 ◽  
Vol 68 (8) ◽  
pp. 4531-4538 ◽  
Author(s):  
Brenda A. Wilson ◽  
Lyaylya R. Aminova ◽  
Virgilio G. Ponferrada ◽  
Mengfei Ho
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Pasteurella Multocida ◽  
Morphological Changes ◽  
Cell Types ◽  
Vero Cells ◽  
3T3 Cells ◽  
Mitogenic Response ◽  
Cycle Progression ◽  
Swiss 3T3

ABSTRACT The intracellularly acting protein toxin of Pasteurella multocida (PMT) causes numerous effects in cells, including activation of inositol 1,4,5-trisphosphate (IP3) signaling, Ca2+ mobilization, protein phosphorylation, morphological changes, and DNA synthesis. The direct intracellular target of PMT responsible for activation of the IP3 pathway is the Gq/11α-protein, which stimulates phospholipase C (PLC) β1. The relationship between PMT-mediated activation of the Gq/11-PLC-IP3pathway and its ability to promote mitogenesis and cellular proliferation is not clear. PMT stimulation of p42/p44 mitogen-activated protein kinase occurs upstream via Gq/11-dependent transactivation of the epidermal growth factor receptor. We have further characterized the effects of PMT on the downstream mitogenic response and cell cycle progression in Swiss 3T3 and Vero cells. PMT treatment caused dramatic morphological changes in both cell lines. In Vero cells, limited multinucleation, nuclear fragmentation, and disruption of cytokinesis were also observed; however, a strong mitogenic response occurred only with Swiss 3T3 cells. Significantly, this mitogenic response was not sustained. Cell cycle analysis revealed that after the initial mitogenic response to PMT, both cell types subsequently arrested primarily in G1and became unresponsive to further PMT treatment. In Swiss 3T3 cells, PMT induced up-regulation of c-Myc; cyclins D1, D2, D3, and E; p21; PCNA; and the Rb proteins, p107 and p130. In Vero cells, PMT failed to up-regulate PCNA and cyclins D3 and E. We also found that the initial PMT-mediated up-regulation of several of these signaling proteins was not sustained, supporting the subsequent cell cycle arrest. The consequences of PMT entry thus depend on the differential regulation of signaling pathways within different cell types.

scholarly journals Escherichia coli Cytotoxic Necrotizing Factor andPasteurella multocida Toxin Induce Focal Adhesion Kinase Autophosphorylation and Src Association

2001 ◽  
Vol 69 (9) ◽  
pp. 5931-5935 ◽  
Author(s):  
Warren Thomas ◽  
Gillian D. Pullinger ◽  
Alistair J. Lax ◽  
Enrique Rozengurt
Keyword(s):  
Escherichia Coli ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Pasteurella Multocida ◽  
Family Members ◽  
Time Dependent ◽  
3T3 Cells ◽  
Cytotoxic Necrotizing Factor ◽  
Swiss 3T3 ◽  
Cytotoxic Necrotizing Factor 1

ABSTRACT Cytotoxic necrotizing factor 1 and Pasteurella multocida toxin induced dose- and time-dependent increases in focal adhesion kinase (FAK) Tyr397 phosphorylation in Swiss 3T3 cells. FAK autophosphorylation was sensitive to inhibitors of p160/ROCK and coincided with the formation of stable complexes between FAK and Src family members.

scholarly journals Calcium stimulation of plasminogen activator secretion/production by swiss 3T3 cells.

1977 ◽  
Vol 252 (18) ◽  
pp. 6256-6259
Author(s):  
I N Chou ◽  
R O Roblin ◽  
P H Black
Keyword(s):  
Plasminogen Activator ◽  
3T3 Cells ◽  
Calcium Stimulation ◽  
Swiss 3T3 ◽  
Stimulation Of
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