Transcriptomic analysis of the phytotoxic effects of 1-allyl-3-methylimidazolium chloride on the growth and plant hormone metabolic pathways of maize (Zea mays L.) seedlings

In order to map the genes conditioning the induction of embryos during our anther culture process, we evaluated F2 plants from three different crosses for their anther culture ability and also performed RFLP analysis on these plants. The results showed that six chromosomal regions appear to be associated with the ability to induce embryo-like structures from maize microspores. These regions are located on chromosomes 1 (two regions), 3, 5, 7, and 8. Some of these chromosomes are identical to those found in previous studies and we have localized the regions more precisely. Notably, all chromosome regions identified, except one, are near viviparous mutant loci. Since the viviparous mutations are known to involve the plant hormone abscisic acid (ABA), these results suggest that ABA or its antagonist, gibberellic acid (GA3), might somehow be related to anther culture ability. We also propose some combinations of probes to screen for anther culture ability in the three genotypes studied.Key words: restriction fragment length polymorphism, anther culture, gene mapping, Zea mays L.

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Phytotoxic effects of two commonly used laundry detergents on germination, growth, and biochemical characteristics of maize (Zea mays L.) seedlings

Environmental Monitoring and Assessment ◽

10.1007/s10661-018-7031-6 ◽

2018 ◽

Vol 190 (11) ◽

Cited By ~ 1

Author(s):

Syeda Uzma ◽

Sarzamin Khan ◽

Waheed Murad ◽

Nadia Taimur ◽

Azizullah Azizullah

Keyword(s):

Zea Mays ◽

Zea Mays L ◽

Biochemical Characteristics ◽

Laundry Detergents ◽

Phytotoxic Effects

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Transcriptome analysis reveals that multiple metabolic pathways operate in Zea mays roots subjected to graphene

10.21203/rs.3.rs-29027/v1 ◽

2020 ◽

Author(s):

Zhiwen Chen ◽

Jianguo Zhao ◽

Jie Song ◽

Shenghua Han ◽

Yaqin Du ◽

...

Keyword(s):

Zea Mays ◽

Metabolic Pathways ◽

Plant Hormone ◽

Control Group ◽

Maize Seedlings ◽

Root Tips ◽

Growth Indices ◽

Root Volume ◽

Maize Roots ◽

Potassium Metabolism

Abstract Background: To explore the effects and molecular mechanism of graphene on the growth and development of Zea mays L., the seeds were randomly divided into the control and experimental groups in this study, the roots of Zea mays L. seedlings were watered by different concentrations (0~100 mg/L) graphene. Results: By evaluating the root growth indices of maize, 50 mg/L graphene increased significantly the total root length, root volume, the number of root tips and root forks of maize seedlings compared with the control group. The contents of nitrogen and potassium in the soil around the roots were elevated after the treatment of 50 mg/L graphene. Then, we compared the transcriptome changes of Zea mays roots in response to 50 mg/L graphene treatment. Transcriptional factor regulation, plant hormone signal transduction, nitrogen and potassium metabolism as well as secondary metabolism in maize roots subjected to graphene showed significant up-regulated expressions, all of which might be related to mechanisms underlying graphene response. Based on qPCR validations, we proposed several candidate genes that might be responded to the graphene treatment in maize roots. Conclusion: The transcriptional profiles presented here provide a foundation for deciphering the mechanism between the graphene and maize roots interaction.

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Leaf apoplastic alkalisation promotes transcription of the ABA synthesising enzyme Vp14 and stomatal closure in Zea mays L

Journal of Experimental Botany ◽

10.1093/jxb/eraa589 ◽

2020 ◽

Author(s):

Christoph-Martin Geilfus ◽

Xudong Zhang ◽

Axel Mithöfer ◽

Lisa Burgel ◽

Gyöngyi Bárdos ◽

...

Keyword(s):

Zea Mays ◽

Plant Hormone ◽

Zea Mays L ◽

Stomatal Closure ◽

Stomatal Aperture ◽

Induced Responses ◽

In Planta ◽

Qrt Pcr ◽

Chloride Salinity ◽

Leaf Apoplast

Abstract The chloride component of NaCl-salinity causes the leaf apoplast to transiently alkalinize. This transition in pH reduces stomatal aperture. However, whether this apoplastic pH (pHapo) transient initiates stomatal closure by interacting with other chloride-stress-induced responses or whether the pH transient alone initiates stomatal closure is unknown. To clarify the problem, the transient alkalinization of the leaf apoplast was mimicked in intact maize (Zea mays L.) by infiltrating near-neutral pH buffers into the leaf apoplast. Effects of the pHapo transient could thus be investigated independently from other chloride-stress-derived effects. Microscopy-based ratiometric live pHapo-imaging was used to monitor pHapo in planta. LC-MS/MS and real-time-qRT-PCR leaf analyses showed that the artificially induced pHapo transient led to an increase in the concentrations of the stomata-regulating plant hormone abscisic acid (ABA) and in transcripts of the key ABA-synthesizing gene ZmVp14 in the leaf. Since stomatal aperture and stomatal conductance decreased according to pHapo, we conclude that the pHapo transient alone initiates stomatal closure. Therefore, the functionality does not depend on interactions with other compounds induced by chloride-stress. Overall, our data indicate that the pH of the leaf apoplast links chloride-salinity with the control of stomatal aperture via effects exerted on the transcription of ABA.

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