Preparation of high efficiency and low carry-over immobilized enzymatic reactor with methacrylic acid–silica hybrid monolith as matrix for on-line protein digestion

2014 ◽  
Vol 1371 ◽  
pp. 48-57 ◽  
Author(s):  
Huiming Yuan ◽  
Lihua Zhang ◽  
Yukui Zhang
Keyword(s):  
Methacrylic Acid ◽  
High Efficiency ◽  
Protein Digestion ◽  
On Line ◽  
Carry Over ◽  
Enzymatic Reactor ◽  
Silica Hybrid

High-Efficiency On-Line Solid-Phase Extraction Coupling to 15−150-μm-i.d. Column Liquid Chromatography for Proteomic Analysis

2003 ◽  
Vol 75 (14) ◽  
pp. 3596-3605 ◽  
Author(s):  
Yufeng Shen ◽  
Ronald J. Moore ◽  
Rui Zhao ◽  
Josip Blonder ◽  
Deanna L. Auberry ◽  
...  
Keyword(s):  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Proteomic Analysis ◽  
High Efficiency ◽  
Solid Phase ◽  
Phase Extraction ◽  
On Line

scholarly journals Improved CUT&RUN chromatin profiling and analysis tools

2019 ◽  
Author(s):  
Michael P. Meers ◽  
Terri Bryson ◽  
Steven Henikoff
Keyword(s):  
Fusion Protein ◽  
High Efficiency ◽  
Low Cost ◽  
Protein A ◽  
Micrococcal Nuclease ◽  
Permeabilized Cells ◽  
E Coli ◽  
Carry Over ◽  
Chromatin Profiling ◽  
Premature Release

AbstractWe previously described a novel alternative to Chromatin Immunoprecipitation, Cleavage Under Targets & Release Using Nuclease (CUT&RUN), in which unfixed permeabilized cells are incubated with antibody, followed by binding of a Protein A-Micrococcal Nuclease (pA/MNase) fusion protein (1). Upon activation of tethered MNase, the bound complex is excised and released into the supernatant for DNA extraction and sequencing. Here we introduce four enhancements to CUT&RUN: 1) a hybrid Protein A-Protein G-MNase construct that expands antibody compatibility and simplifies purification; 2) a modified digestion protocol that inhibits premature release of the nuclease-bound complex; 3) a calibration strategy based on carry-over of E. coli DNA introduced with the fusion protein; and 4) a novel peak-calling strategy customized for the low-background profiles obtained using CUT&RUN. These new features, coupled with the previously described low-cost, high efficiency, high reproducibility and high-throughput capability of CUT&RUN make it the method of choice for routine epigenomic profiling.

scholarly journals On-line protein digestion by immobilized enzyme microreactor capillary electrophoresis-mass spectrometry

Talanta ◽  
2019 ◽  
Vol 199 ◽  
pp. 116-123 ◽  
Author(s):  
Lorena Villegas ◽  
Roger Pero-Gascon ◽  
Fernando Benavente ◽  
José Barbosa ◽  
Victoria Sanz-Nebot
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Immobilized Enzyme ◽  
Protein Digestion ◽  
On Line ◽  
Capillary Electrophoresis Mass Spectrometry

Vinyl functionalized silica hybrid monolith-based trypsin microreactor for on line digestion and separation via thiol-ene “click” strategy

2011 ◽  
Vol 1218 (44) ◽  
pp. 7982-7988 ◽  
Author(s):  
Yingzhuang Chen ◽  
Minghuo Wu ◽  
Keyi Wang ◽  
Bo Chen ◽  
Shouzhuo Yao ◽  
...  
Keyword(s):  
Functionalized Silica ◽  
On Line ◽  
Silica Hybrid

Design of On-Line Detection System for Paper Defects Based on Line-Scan Camera

2014 ◽  
Vol 536-537 ◽  
pp. 268-271
Author(s):  
Ping He ◽  
Chao Liu ◽  
Meng Wang ◽  
Sheng Mei Cao
Keyword(s):  
High Efficiency ◽  
Detection System ◽  
Low Cost ◽  
Digital Camera ◽  
Line Detection ◽  
Detection Scheme ◽  
Line Scan ◽  
On Line ◽  
Increasing Demand ◽  
Technology Level

Paper defects mean that there are some defects in the paper such as hole, emboss, and fold during the paper production, which mainly results from the limitation of technological level. In the past time, artificial visual inspection and off-line checking were often used to detect the paper defects. However, its shortcoming was highlighted along with the improvement of industrial technology level and increasing demand for paper. In order to realize the online detection and markers for paper defects, the project designs the on-line detection system based on line-scan digital camera. Firstly, the principle and detection scheme of the system for the paper defects was presented. Then the overall structure of the system was designed. After that, the hardware circuit of the system was designed using TMS320F2812 as main control chip. It mainly consists of the function of each module and the working process of the system. Finally, the software of the image acquisition system was presented. With the experimental verification, the system has advantages of low cost, high efficiency and strong resistance to interference. The functions and indexes achieved the design requirements.

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