More than 170 polyunsaturated tocopherol-related compounds in a vitamin E capsule: Countercurrent chromatographic enrichment, gas chromatography/mass spectrometry analysis and preliminary identification of the potential artefacts

2016 ◽  
Vol 1476 ◽  
pp. 77-87 ◽  
Author(s):  
Simon Hammann ◽  
Alexander Kröpfl ◽  
Walter Vetter
Author(s):  
Shahina Parveen ◽  
Anwar Shahzad ◽  
Anamica Upadhyay ◽  
Vikas Yadav

ABSTRACTObjective: The leaves of Cassia angustifolia Vahl. are employed for the treatment of several human diseases. Therefore, the present study wasundertaken to determine the phytocomponents present in the methanolic extract of the leaves by gas chromatography-mass spectrometry (GC-MS)Methods: The collected leaf samples were dried and extracted in methanol. Screening of the extract was done by GC-MS which is an importanttechnique for the separation and identification of different phytochemicals.Results: The methanolic extract of the leaves of C. angustifolia revealed the presence of 45 different phytochemicals. The prevailing compounds were1 butanol, 3 methyl acetate (area % 7.47), 6, 6-dideutero-nonen-1-Ol-3 (area % 10.45), pentadecanoic acid (area % 9.22), and squalene (area %12.30). Vitamin E (area % 3.85) has also been found in the leaf extract. Some of the compounds possess biological activities.Conclusions: It can be concluded from the present study that some of the identified phytochemicals could be responsible for the medicinal value orbiological activity of the plant leaves.Keywords: Senna, Leaf extract, Methanol, Gas chromatography-mass spectrometry, Phytochemicals, Vitamin E.


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