A high-level theoretical characterization of the electronic states and spectroscopic parameters of SrBr2+ and SrI2+, and thermodynamic stability in the family of strontium monohalides dications

2019 ◽  
Vol 722 ◽  
pp. 12-17 ◽  
Author(s):  
Gabriel Fernando de Melo ◽  
Fernando R. Ornellas
Keyword(s):  
Thermodynamic Stability ◽  
Electronic States ◽  
Spectroscopic Parameters ◽  
The Family ◽  
Theoretical Characterization ◽  
High Level

Cloning and expression of DiT33 from Dirofilaria immitis: a specific and early marker of heartworm infection

Parasitology ◽  
1996 ◽  
Vol 112 (3) ◽  
pp. 331-338 ◽  
Author(s):  
X. Q. Hong ◽  
J. Santiago Mejia ◽  
S. Kumar ◽  
F. B. Perler ◽  
C. K. S. Carlow
Keyword(s):  
Dirofilaria Immitis ◽  
Cloning And Expression ◽  
E Coli ◽  
Spliced Leader ◽  
Isolation And Characterization ◽  
The Family ◽  
Heartworm Infection ◽  
High Level ◽  
Control And Management

SUMMARYDirofilaria immitis is an important filarial parasite of dogs and cats, and a useful model for human filariasis. Current diagnostic tests for heartworm infection in animals rely on the presence of fecund female worms (usually found 6·5 months post-infection or later) and therefore fail to detect pre-patent infections. Putative pepsin inhibitors from 2 filarial parasites of humans namely Onchocerca volvulus (Ov33, Oc3.6, OvDSB) and Brugia malayi (Bm33), have been shown to be useful in diagnosis of onchocerciasis and lymphatic filariasis, respectively. Previous studies have suggested that a homologue exists in D. immitis (DiT33), which may have potential in diagnosis of heartworm infection. In this study, the isolation and characterization of a cDNA clone encoding DiT33 is described.‡ This cDNA contains 12 bases of the nematode-specific 22 nucleotide spliced leader sequence and encodes a 26·4 kDa-protein with a high level of similarity (87–89%) to other filarial members of the family. DJT33 was over-expressed in E. coli as a fusion with the maltose-binding protein and serological analysis was performed using a panel of clinically defined dog sera. The findings of this study indicate that DiT33 is a promising antigen for the early detection of D. immitis and may be a valuable tool in the control and management of heartworm infection.

Theoretical characterization of low-lying electronic states of FCO

1992 ◽  
Vol 191 (1-2) ◽  
pp. 13-22 ◽  
Author(s):  
Joseph S. Francisco ◽  
Avery N. Goldstein ◽  
Michael A. Robb ◽  
Ian H. Williams
Keyword(s):  
Electronic States ◽  
Theoretical Characterization

scholarly journals Psychroserpens mesophilus sp. nov., a mesophilic marine bacterium belonging to the family Flavobacteriaceae isolated from a young biofilm

2006 ◽  
Vol 56 (5) ◽  
pp. 1055-1058 ◽  
Author(s):  
Kae Kyoung Kwon ◽  
Soon Jae Lee ◽  
Jae Hyun Park ◽  
Tae-Young Ahn ◽  
Hong Kum Lee
Keyword(s):  
Marine Bacterium ◽  
Methyl Esters ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
The Family ◽  
Major Respiratory Quinone ◽  
Dominant Fatty Acid ◽  
High Level ◽  
Taxonomic Characterization

A number of marine bacteria isolated from young biofilms were characterized as belonging to the family Flavobacteriaceae. The taxonomic characterization of strain KOPRI 13649T, which was isolated from an acrylic surface at the seashore at Gangneung, Korea, is reported here. The nearly complete 16S rRNA gene sequence of strain KOPRI 13649T was determined and was found to have a high level of similarity with that of Psychroserpens burtonensis (95.0–95.6 %). In addition, phylogenetic analysis and comparison with closely related strains confirmed that the strain represented a novel member of the genus Psychroserpens. The major respiratory quinone of strain KOPRI 13649T was MK-6 and the DNA G+C content was 29.8 mol%. The dominant fatty acid methyl esters were i-15 : 0, a-15 : 0, i-16 : 0, i-15 : 1ω10, 16 : 1ω7 and 15 : 0. Growth was observed at 10–34 °C (optimum 30 °C), at pH 6–9 (optimum 6.5–8.0) and with 0.5–4 % NaCl (optimum 1 %). On the basis of the polyphasic taxonomic evidence presented, strain KOPRI 13649T (=KCCM 42261T=JCM 13413T) should be classified as the type strain of a novel species in the genus Psychroserpens, for which the name Psychroserpens mesophilus sp. nov. is proposed.

scholarly journals Morphological and molecular characterization of Gordionus alpestris (Nematomorpha) from the North-West Caucasus, Russia

2013 ◽  
Vol 50 (1) ◽  
pp. 67-72 ◽  
Author(s):  
S. Spiridonov ◽  
V. Schmatko
Keyword(s):  
Molecular Characterization ◽  
North West ◽  
The North ◽  
The Family ◽  
Pcr Products ◽  
High Level ◽  
Morphological And Molecular Characterization

AbstractDuring the survey of Pachyiulus krivolutskyi Golovatch, 1977 (Diplopoda) near Nickel’ in Adygei Republic (Russia) in the summer 2009 the infestation of these millipeds with nematomorphs of the species Gordionus alpestris was revealed. The morphology of naturally emerging nematomorphs was studied in SEM. The partial sequences of 18S (883 bp) and 28S (393 bp) rDNA PCR-products were obtained and analyzed. An unexpectedly high level of differences between Gordionus alpestris found in diplopods of North-West Caucasus and other molecularly studied representatives of the family Chordodidae was observed.

scholarly journals High-level theoretical characterization of the vinoxy radical (•CH2CHO) + O2 reaction

2018 ◽  
Vol 148 (18) ◽  
pp. 184308 ◽  
Author(s):  
Jared D. Weidman ◽  
Ryan T. Allen ◽  
Kevin B. Moore ◽  
Henry F. Schaefer
Keyword(s):  
Theoretical Characterization ◽  
High Level

Theoretical characterization of the low-lying electronic states of NbC

2005 ◽  
Vol 123 (5) ◽  
pp. 054318 ◽  
Author(s):  
Pablo A. Denis ◽  
K. Balasubramanian
Keyword(s):  
Electronic States ◽  
Theoretical Characterization

scholarly journals Characterization of the Salmonella bacteriophage vB_SenS-Ent1

2012 ◽  
Vol 93 (9) ◽  
pp. 2046-2056 ◽  
Author(s):  
Dann Turner ◽  
Mohammed Hezwani ◽  
Shona Nelson ◽  
Vyv Salisbury ◽  
Darren Reynolds
Keyword(s):  
Nucleotide Sequence Identity ◽  
Burst Size ◽  
Genome Sequences ◽  
Sequence Identity ◽  
The Family ◽  
Contractile Tail ◽  
Transcriptional Units ◽  
High Level ◽  
Icosahedral Head

The bacteriophage vB_SenS-Ent1 (Ent1) is a member of the family Siphoviridae of tailed bacteriophages and infects a broad range of serovars of the enteric pathogen Salmonella enterica. The virion particle is composed of an icosahedral head 64 nm in diameter and a flexible, non-contractile tail of 116 × 8.5 nm possessing terminal fibres. The adsorption rate constant at 37 °C is 6.73 × 10−9 ml min−1. Latent and eclipse periods are 25 and 20 min, respectively, and the burst size is 35 progeny particles per cell after 35 min at 37 °C. Sequencing revealed a circularly permuted, 42 391 bp dsDNA genome containing 58 ORFs organized into four major transcriptional units. Comparisons with the genome sequences of other bacteriophages revealed a high level of nucleotide sequence identity and shared orthologous proteins with the Salmonella phages SETP3, SE2 and KS7 (SS3e) and the Escherichia phages K1G, K1H, K1ind1 and K1ind3.

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