Comparison of results of fluconazole and voriconazole disk diffusion testing for Candida spp. with results from a central reference laboratory in the ARTEMIS DISK Global Antifungal Surveillance Program

2009 ◽  
Vol 65 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Michael A. Pfaller ◽  
Linda Boyken ◽  
Richard J. Hollis ◽  
Jennifer Kroeger ◽  
Shawn A. Messer ◽  
...  
Keyword(s):  
Disk Diffusion ◽  
Surveillance Program ◽  
Reference Laboratory ◽  
Candida Spp ◽  
Comparison Of Results ◽  
Disk Diffusion Testing

scholarly journals In Vitro Activities of Fluconazole and Voriconazole against Clinical Isolates of Candida spp. Determined by Disk Diffusion Testing in Turin, Italy

2009 ◽  
Vol 53 (4) ◽  
pp. 1657-1659 ◽  
Author(s):  
Narcisa Mandras ◽  
Vivian Tullio ◽  
Valeria Allizond ◽  
Daniela Scalas ◽  
Giuliana Banche ◽  
...  
Keyword(s):  
Clinical Isolates ◽  
Disk Diffusion ◽  
In Vitro Activity ◽  
High Susceptibility ◽  
Diffusion Test ◽  
Candida Spp ◽  
Disk Diffusion Test ◽  
Disk Diffusion Testing ◽  
Fluconazole Resistant

ABSTRACT The in vitro activities of fluconazole and voriconazole against 1,024 clinical isolates of Candida spp. were determined by the agar disk diffusion test using the Clinical and Laboratory Standards Institute (CLSI) M44-A guidelines. The results of this investigation demonstrated the broad-spectrum in vitro activity of voriconazole, relative to that of fluconazole, against yeasts tested, in particular fluconazole-resistant isolates, such as Candida krusei that showed high susceptibility to voriconazole. The situation in Turin, Italy, is quite similar to that of the rest of Italy, reflecting the worldwide trend.

scholarly journals High-Level Neisseria gonorrhea Resistance Detected in a Newly Implemented Surveillance Program in Kampala, Uganda

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S103-S103
Author(s):  
Meklit Workneh ◽  
Mohammed Lamorde ◽  
Francis Kakooza ◽  
Olive Mbabazi ◽  
Rodney Mugasha ◽  
...  
Keyword(s):  
National Policy ◽  
Antibiotic Use ◽  
Empiric Therapy ◽  
Disk Diffusion ◽  
Surveillance Program ◽  
Reference Laboratory ◽  
Urethral Discharge ◽  
Early Results ◽  
Neisseria Gonorrhea ◽  
Ongoing Surveillance

Abstract Background Neisseria gonorrhea resistance is a growing problem in Uganda with recent data showing increasing ciprofloxacin resistance up to 100% in this population. The WHO Enhanced Gonococcal Antimicrobial Surveillance Programme (EGASP) was initiated in Uganda in September 2016 to monitor resistance trends. Methods Urethral swabs were collected from men presenting with urethral discharge to the five sentinel clinic sites from September 2016 to March 2017. Samples were transported to a reference laboratory site. Presumptive identification of N. gonorrhea was based on growth of typical appearing colonies on Thayer–Martin in 5% CO2, a positive oxidase test, and observation of Gram-negative, oxidase-positive diplococci in stained smears. Results 116 samples were received to the reference laboratory site of which 70 (60.3%) had positive growth for Neisseria gonorrhea. Mean age was 28.5 (range 17–60). Fifty-one participants (44%) reported at least one prior episode of gonorrhea and 42 (36%) reported antibiotic use within the previous 60 days. Of those with completed Etest (bioMerieux, Marcy-lÕEtoile, France) resistance profiles, 66 (96%) were ciprofloxacin-resistant or intermediate. One isolate was ceftriaxone-resistant by E-test but susceptible by disk diffusion. Conclusion Early results from implementation of a gonorrhea surveillance program in Uganda suggest high levels of resistance to ciprofloxacin (90%) by Etest and penicillin (93%) and tetracyclines (100%) by disk diffusion. Prior studies of gonococcal resistance in Uganda have noted increasing levels of resistance, particularly to ciprofloxacin which until 2010 was the recommended first-line empiric therapy for gonococcal infection in Uganda. Of note, discrepancies were occasionally noted between disk diffusion and Etest results, which requires further investigation. Ongoing surveillance efforts will be crucial to shape clinical guidelines and national policy. Disclosures All authors: No reported disclosures.

In vitro susceptibilities of bloodstream isolates of Candida spp.: results from a multicenter active surveillance program in Andalusia

2010 ◽  
Vol 28 (6) ◽  
pp. 405
Author(s):  
Carmen Flórez ◽  
Estrella Martín-Mazuelos ◽  
Maite Ruiz ◽  
José Miguel Cisneros ◽  
Marta Herrero ◽  
...  
Keyword(s):  
Active Surveillance ◽  
Surveillance Program ◽  
Candida Spp ◽  
Active Surveillance Program

scholarly journals Complete ciprofloxacin resistance in gonococcal isolates in an urban Ugandan clinic: findings from a cross-sectional study

2018 ◽  
Vol 30 (3) ◽  
pp. 256-263 ◽  
Author(s):  
Emily Mabonga ◽  
Rosalind Parkes-Ratanshi ◽  
Stefan Riedel ◽  
Sheila Nabweyambo ◽  
Olive Mbabazi ◽  
...  
Keyword(s):  
Sex Workers ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Outpatient Service ◽  
Tetracycline Resistance ◽  
Disk Diffusion ◽  
Reference Laboratory ◽  
Transmitted Infection ◽  
Cross Sectional ◽  
Agar Dilution

Antimicrobial resistance (AMR) to gonorrhoea is a threat to global health security. There have been concerns expressed that countries with high rates of disease have poor surveillance. The objectives of the study were to determine the AMR patterns of Neisseria gonorrhoeae clinical isolates to antimicrobial agents in patients with HIV or high risk of HIV acquisition, to compare the concordance of disk diffusion and agar dilution as methods for determining AMR to N. gonorrhoeae, and to describe methodological challenges to carrying out AMR testing. The study was conducted at an HIV outpatient service for at-risk populations and an outreach clinic for commercial sex workers in Kampala. Patients were offered a sexually transmitted infection screen using a polymerase chain reaction (PCR)-based assay. Samples positive for gonorrhoea were cultured. Antimicrobial susceptibility testing was performed using disk diffusion and isolates were sent to a reference laboratory for agar dilution direct susceptibility testing. Five hundred and seventy-five patients were screened. There were 33 (5.7%) patients with gonorrhoea detected by PCR. Of the 16 viable N. gonorrhoeae isolates, 100% were resistant to ciprofloxacin and tetracycline by disk diffusion and 31% exhibited reduced susceptibility to ceftriaxone and cefixime. By agar dilution, 100% of isolates were resistant to ciprofloxacin and all isolates were susceptible to ceftriaxone and cefixime. There was concordance between disk diffusion and agar dilution for ciprofloxacin and tetracycline resistance and a significant discordance for third-generation cephalosporins. More than half the women with gonorrhoea were asymptomatic and represent a potential reservoir for ongoing transmission. AMR testing of N. gonorrhoeae isolates is needed to ensure optimal treatment and prevention of antibiotic resistance progression.

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